ID A4T7K7_MYCGI Unreviewed; 427 AA.
AC A4T7K7;
DT 15-MAY-2007, integrated into UniProtKB/TrEMBL.
DT 15-MAY-2007, sequence version 1.
DT 01-MAY-2013, entry version 51.
DE RecName: Full=Phosphoribosylamine--glycine ligase;
DE EC=6.3.4.13;
DE AltName: Full=GARS;
DE AltName: Full=Glycinamide ribonucleotide synthetase;
DE AltName: Full=Phosphoribosylglycinamide synthetase;
GN Name=purD; OrderedLocusNames=Mflv_1608;
OS Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens
OS (strain ATCC 700033 / PYR-GCK)).
OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC Corynebacterineae; Mycobacteriaceae; Mycobacterium.
OX NCBI_TaxID=350054;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PYR-GCK;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H.,
RA Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Miller C.,
RA Richardson P.;
RT "Complete sequence of chromosome of Mycobacterium gilvum PYR-GCK.";
RL Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY: ATP + 5-phospho-D-ribosylamine + glycine = ADP
CC + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.
CC -!- COFACTOR: Binds (magnesium or manganese,ion) per subunit (By
CC similarity).
CC -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway;
CC N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-
CC ribose 1-diphosphate: step 2/2.
CC -!- SIMILARITY: Belongs to the GARS family.
CC -!- SIMILARITY: Contains 1 ATP-grasp domain.
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DR EMBL; CP000656; ABP44090.1; -; Genomic_DNA.
DR RefSeq; YP_001132878.1; NC_009338.1.
DR ProteinModelPortal; A4T7K7; -.
DR STRING; 350054.Mflv_1608; -.
DR EnsemblBacteria; ABP44090; ABP44090; Mflv_1608.
DR GeneID; 4972934; -.
DR KEGG; mgi:Mflv_1608; -.
DR PATRIC; 18031281; VBIMycGil17082_1644.
DR eggNOG; COG0151; -.
DR HOGENOM; HOG000033463; -.
DR KO; K01945; -.
DR OMA; MGAYTPL; -.
DR ProtClustDB; PRK00885; -.
DR BioCyc; MGIL350054:GHK8-2005-MONOMER; -.
DR UniPathway; UPA00074; UER00125.
DR GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR GO; GO:0000287; F:magnesium ion binding; IEA:HAMAP.
DR GO; GO:0030145; F:manganese ion binding; IEA:HAMAP.
DR GO; GO:0004637; F:phosphoribosylamine-glycine ligase activity; IEA:HAMAP.
DR GO; GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0009113; P:purine nucleobase biosynthetic process; IEA:InterPro.
DR Gene3D; 3.30.1490.20; -; 1.
DR Gene3D; 3.30.470.20; -; 1.
DR Gene3D; 3.40.50.20; -; 1.
DR Gene3D; 3.90.600.10; -; 1.
DR HAMAP; MF_00138; GARS; 1; -.
DR InterPro; IPR011761; ATP-grasp.
DR InterPro; IPR013815; ATP_grasp_subdomain_1.
DR InterPro; IPR013816; ATP_grasp_subdomain_2.
DR InterPro; IPR016185; PreATP-grasp_dom.
DR InterPro; IPR020561; PRibGlycinamid_synth_ATP-grasp.
DR InterPro; IPR000115; PRibGlycinamide_synth.
DR InterPro; IPR020560; PRibGlycinamide_synth_C-dom.
DR InterPro; IPR020559; PRibGlycinamide_synth_CS.
DR InterPro; IPR020562; PRibGlycinamide_synth_N.
DR InterPro; IPR011054; Rudment_hybrid_motif.
DR Pfam; PF01071; GARS_A; 1.
DR Pfam; PF02843; GARS_C; 1.
DR Pfam; PF02844; GARS_N; 1.
DR SUPFAM; SSF52440; PreATP-grasp-like; 1.
DR SUPFAM; SSF51246; Rudmnt_hyb_motif; 1.
DR TIGRFAMs; TIGR00877; purD; 1.
DR PROSITE; PS50975; ATP_GRASP; 1.
DR PROSITE; PS00184; GARS; 1.
PE 3: Inferred from homology;
KW ATP-binding; Complete proteome; Ligase; Magnesium; Manganese;
KW Metal-binding; Nucleotide-binding; Purine biosynthesis.
FT DOMAIN 107 312 ATP-grasp (By similarity).
FT NP_BIND 133 193 ATP (By similarity).
FT METAL 282 282 Magnesium or manganese (By similarity).
FT METAL 284 284 Magnesium or manganese (By similarity).
SQ SEQUENCE 427 AA; 43257 MW; 106263D9A97AD321 CRC64;
MHVLVVGSGA REHALLLALR RDPEVDALSV APGNAGTSAV ADQYDLDVTS GDAVVALARR
IGADLVVIGP EVPLVLGVAD AVRAAGIACF GPSKDAARIE GSKAFAKDVM SAAGVRTAGS
EIVDNPAHLD AALDRFGPPA GQAAWVVKDD GLAAGKGVVV TTDRDAARAH AAGLLDAGHP
VLLESFLDGP EVSLFCVVDG DTVVPLLPAQ DFKRVGDGDT GPNTGGMGAY APLPWLPDEV
TKQIVDEIVK PVAAEMVRRG SSFSGLLYAG LAITSRGPAV VEFNCRFGDP ETQAVLALLE
SPLGRLLHAA ATGELAAQPP LHWQDGSAVT VVVAAENYPG RPRVGDVIHG ADAPAVLHAG
TARRDDGSVV SSGGRVLSVV GTGKDLAAAR ESAYAAVAAI RLPGSHFRSD IGQAAAEGRI
SPVGGSG
//
