ID A8ARF9_CITK8 Unreviewed; 676 AA.
AC A8ARF9;
DT 23-OCT-2007, integrated into UniProtKB/TrEMBL.
DT 23-OCT-2007, sequence version 1.
DT 24-JAN-2024, entry version 80.
DE RecName: Full=Glycosyl hydrolase family 13 catalytic domain-containing protein {ECO:0000259|SMART:SM00642};
GN OrderedLocusNames=CKO_05029 {ECO:0000313|EMBL:ABV16072.1};
OS Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Citrobacter.
OX NCBI_TaxID=290338 {ECO:0000313|EMBL:ABV16072.1, ECO:0000313|Proteomes:UP000008148};
RN [1] {ECO:0000313|EMBL:ABV16072.1, ECO:0000313|Proteomes:UP000008148}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-895 / CDC 4225-83 / SGSC4696
RC {ECO:0000313|Proteomes:UP000008148};
RG The Citrobacter koseri Genome Sequencing Project;
RA McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S.,
RA Latreille P., Courtney L., Wang C., Pepin K., Bhonagiri V., Nash W.,
RA Johnson M., Thiruvilangam P., Wilson R.;
RL Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|PIRSR:PIRSR036917-2};
CC Note=Binds 1 Ca(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR036917-2};
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DR EMBL; CP000822; ABV16072.1; -; Genomic_DNA.
DR RefSeq; WP_012135710.1; NC_009792.1.
DR AlphaFoldDB; A8ARF9; -.
DR STRING; 290338.CKO_05029; -.
DR CAZy; GH13; Glycoside Hydrolase Family 13.
DR GeneID; 45138491; -.
DR KEGG; cko:CKO_05029; -.
DR HOGENOM; CLU_022115_1_0_6; -.
DR OMA; DKVMVVW; -.
DR OrthoDB; 9805159at2; -.
DR Proteomes; UP000008148; Chromosome.
DR GO; GO:0042597; C:periplasmic space; IEA:InterPro.
DR GO; GO:0004556; F:alpha-amylase activity; IEA:InterPro.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0030980; P:alpha-glucan catabolic process; IEA:InterPro.
DR GO; GO:0009313; P:oligosaccharide catabolic process; IEA:InterPro.
DR Gene3D; 3.20.20.80; Glycosidases; 2.
DR InterPro; IPR014635; A_amylase_MalS.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR10357; ALPHA-AMYLASE FAMILY MEMBER; 1.
DR PANTHER; PTHR10357:SF209; PERIPLASMIC ALPHA-AMYLASE; 1.
DR Pfam; PF00128; Alpha-amylase; 2.
DR PIRSF; PIRSF036917; Alph_amls_MalS; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE 4: Predicted;
KW Calcium {ECO:0000256|PIRSR:PIRSR036917-2};
KW Disulfide bond {ECO:0000256|PIRSR:PIRSR036917-4};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR036917-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000008148};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..17
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 18..676
FT /note="Glycosyl hydrolase family 13 catalytic domain-
FT containing protein"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5002716991"
FT DOMAIN 193..637
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT ACT_SITE 460
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR036917-1"
FT ACT_SITE 503
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR036917-1"
FT BINDING 314
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR036917-2"
FT BINDING 464
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR036917-2"
FT SITE 565
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR036917-3"
FT DISULFID 57..75
FT /evidence="ECO:0000256|PIRSR:PIRSR036917-4"
FT DISULFID 121..537
FT /evidence="ECO:0000256|PIRSR:PIRSR036917-4"
SQ SEQUENCE 676 AA; 75593 MW; 453616EFB3A15255 CRC64;
MKLTAFALAL MPGIVFASSW TSQGFPPFTA EGTGKFVSHA ALTKGTRPLS LHIDQQCWQP
ADAIKLNQML SLKPCEGTPP QWRLFKDGDY SLEIDTRSGT PTLTLSIKST ADPVASTVRQ
CPTWNGSPLT LEVSHTFPEG AVVRDYYSQQ TATVKNGQIT LQPGATSNGL LLLERAETDT
PAAFNWHNAT VYFVLTDRFQ NGDPTNDQSY GRHKDGMEEI GTFHGGDLRG LANKLDYLQH
LGVNALWISA PFEQIHGWVG GGTKGDFPHY AYHGYYTQDW TTLDANMGSE GDLRALVDGA
HQRGIRILFD VVMNHTGYAT LADMQTFRFG ALYLQGDELK KTLGERWTDW KPAAGQSWHS
FNDYINFSDK AAWEAWWGKE WIRTDIGDYD NPGFDDLTMS LAFLPDIKTE STTPSGLPVF
YKNKPDTRAK AIEGYTPRDY LTHWLSQWVR DYGIDGFRVD TAKHVELPAW QQLKTQASAA
LTEWKQANPD KTLDDTPFWM TGEAWGHGVM QSDYYRHGFD AMINFDYQEQ AAKAVDCLAN
MDATWQQMAE KLQDFNVLSY LSSHDTRLFR EGGDKAAELL LLAPGAVQIF YGDESARPFG
PTGSDPLQGA RSDMNWQDIN GKSAATVAHW QRISQFRARH PAIGAGKQTT LSLKQGYGFV
REHDDDKVMV VWAGRP
//