ID   B0JKN0_MICAN            Unreviewed;       820 AA.
AC   B0JKN0;
DT   18-MAR-2008, integrated into UniProtKB/TrEMBL.
DT   18-MAR-2008, sequence version 1.
DT   27-MAR-2024, entry version 77.
DE   RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE            EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN   OrderedLocusNames=MAE_29970 {ECO:0000313|EMBL:BAG02819.1};
OS   Microcystis aeruginosa (strain NIES-843 / IAM M-2473).
OC   Bacteria; Cyanobacteriota; Cyanophyceae; Oscillatoriophycideae;
OC   Chroococcales; Microcystaceae; Microcystis.
OX   NCBI_TaxID=449447 {ECO:0000313|EMBL:BAG02819.1, ECO:0000313|Proteomes:UP000001510};
RN   [1] {ECO:0000313|EMBL:BAG02819.1, ECO:0000313|Proteomes:UP000001510}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NIES-843 {ECO:0000313|EMBL:BAG02819.1,
RC   ECO:0000313|Proteomes:UP000001510};
RX   PubMed=18192279; DOI=10.1093/dnares/dsm026;
RA   Kaneko T., Nakajima N., Okamoto S., Suzuki I., Tanabe Y., Tamaoki M.,
RA   Nakamura Y., Kasai F., Watanabe A., Kawashima K., Kishida Y., Ono A.,
RA   Shimizu Y., Takahashi C., Minami C., Fujishiro T., Kohara M., Katoh M.,
RA   Nakazaki N., Nakayama S., Yamada M., Tabata S., Watanabe M.M.;
RT   "Complete genomic structure of the bloom-forming toxic cyanobacterium
RT   Microcystis aeruginosa NIES-843.";
RL   DNA Res. 14:247-256(2007).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC         N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC         homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC         COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC         Evidence={ECO:0000256|ARBA:ARBA00001279};
CC   -!- SIMILARITY: Belongs to the N(4)/N(6)-methyltransferase family.
CC       {ECO:0000256|ARBA:ARBA00006594}.
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DR   EMBL; AP009552; BAG02819.1; -; Genomic_DNA.
DR   AlphaFoldDB; B0JKN0; -.
DR   STRING; 449447.MAE_29970; -.
DR   REBASE; 17100; M.Mae843ORF29970P.
DR   PaxDb; 449447-MAE_29970; -.
DR   EnsemblBacteria; BAG02819; BAG02819; MAE_29970.
DR   KEGG; mar:MAE_29970; -.
DR   PATRIC; fig|449447.4.peg.2735; -.
DR   eggNOG; COG0827; Bacteria.
DR   HOGENOM; CLU_381660_0_0_3; -.
DR   BioCyc; MAER449447:MAE_RS13075-MONOMER; -.
DR   Proteomes; UP000001510; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:InterPro.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:InterPro.
DR   Gene3D; 3.40.1350.80; -; 1.
DR   Gene3D; 1.10.10.1820; BsuBI/PstI restriction endonuclease-like; 1.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR041963; BsuBI/PstI_C_sf.
DR   InterPro; IPR041454; BsuBI/PstI_N.
DR   InterPro; IPR041962; BsuBI/PstI_N_sf.
DR   InterPro; IPR011639; MethylTrfase_TaqI-like_dom.
DR   InterPro; IPR009528; Restrct_endonuc_II_BsuBI_C.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   Pfam; PF06616; BsuBI_PstI_RE; 1.
DR   Pfam; PF17728; BsuBI_PstI_RE_N; 1.
DR   Pfam; PF07669; Eco57I; 1.
DR   PRINTS; PR00507; N12N6MTFRASE.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE   3: Inferred from homology;
FT   DOMAIN          147..239
FT                   /note="Type II methyltransferase M.TaqI-like"
FT                   /evidence="ECO:0000259|Pfam:PF07669"
FT   DOMAIN          514..646
FT                   /note="BsuBI/PstI restriction endonuclease HTH"
FT                   /evidence="ECO:0000259|Pfam:PF17728"
FT   DOMAIN          661..814
FT                   /note="BsuBI/PstI restriction endonuclease"
FT                   /evidence="ECO:0000259|Pfam:PF06616"
SQ   SEQUENCE   820 AA;  92853 MW;  DE81B562CB8D6716 CRC64;
     MATFLTDSAD IARVYYSSRL NLKQRSQLGQ FLTPATVARF MAGQFNNLSG HIHLLDAGAG
     IGTLTAAVVE RLLANPDQVS SCSITAYEVE PVFFPSLNQT LTECCAALNG KGIQADYCLR
     GENFIKASSE MNLPLFKKVV PGFTHAILNP PYKKIHSQSA EKKVLASIGI DTVNLYSAFV
     WLAIVQLIDD GEVVAITPRS FCNGKYFRPF RKAFLEYMKL DKIHIFESRS ATFSEDEVLQ
     ENIIFHALRS KQKPSTVKIT SNSEMALDEI SESRYTPYDE VIEPNDSEQF IHIVTNSLKN
     SLRVQMNKMP CTLDEIGLEV STGPVVDFRL KSSLRNHLSD RTVPLLYPES VKVRKVVFPP
     DNPRKPIAVE KNNETEKWLI EPGWYVLTKR FSSKEEKRRV VAAVCSPIGS KSLGVENHLN
     YYHAKGRGMP PDVAKGLAAF LNSTLFDSYF RQFSGNTQVN ATDLRRVKYP CKNDLIQIGV
     QVGDNDLNQE EIDQVVHEVL SIMDEASAAV QANKRIEEAL TILKAISAPR AQQNERSALC
     LLALADIQPD KPWSQATAPK RGITEMMDWF RDHYGKQYAP NTRETVRKQT MHQFVQMGLV
     VQNPDKPDRP INSPRWCYQL DRQALSLLQV YGSEQWEEAR RNYALSVTNW LQARNRNLPM
     IPITLPDGRA IQLSSGGQNI LIKDILESFC PRFTPGGVVL YIGDAGDKFI INETQKFREM
     GIELDPHGKM PDLVIYHRYQ DWLVLIEAVT SHGPVNLKRH NELKQLFQLS CKGLVFVTAF
     PSRREMTRYL AEISWETEVW VADQPDHMIH FNGERFLGPY
//