ID   B0X0J0_CULQU            Unreviewed;       357 AA.
AC   B0X0J0;
DT   08-APR-2008, integrated into UniProtKB/TrEMBL.
DT   08-APR-2008, sequence version 1.
DT   27-MAR-2024, entry version 75.
DE   SubName: Full=N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase {ECO:0000313|EMBL:EDS38151.1, ECO:0000313|EnsemblMetazoa:CPIJ012618-PA};
GN   Name=6045879 {ECO:0000313|EnsemblMetazoa:CPIJ012618-PA};
GN   ORFNames=CpipJ_CPIJ012618 {ECO:0000313|EMBL:EDS38151.1};
OS   Culex quinquefasciatus (Southern house mosquito) (Culex pungens).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC   Culicinae; Culicini; Culex; Culex.
OX   NCBI_TaxID=7176;
RN   [1] {ECO:0000313|EMBL:EDS38151.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JHB {ECO:0000313|EMBL:EDS38151.1};
RG   The Broad Institute Genome Sequencing Platform;
RA   Atkinson P.W., Hemingway J., Christensen B.M., Higgs S., Kodira C.,
RA   Hannick L., Megy K., O'Leary S., Pearson M., Haas B.J., Mauceli E.,
RA   Wortman J.R., Lee N.H., Guigo R., Stanke M., Alvarado L., Amedeo P.,
RA   Antoine C.H., Arensburger P., Bidwell S.L., Crawford M., Camaro F.,
RA   Devon K., Engels R., Hammond M., Howarth C., Koehrsen M., Lawson D.,
RA   Montgomery P., Nene V., Nusbaum C., Puiu D., Romero-Severson J.,
RA   Severson D.W., Shumway M., Sisk P., Stolte C., Zeng Q., Eisenstadt E.,
RA   Fraser-Liggett C., Strausberg R., Galagan J., Birren B., Collins F.H.;
RT   "Annotation of Culex pipiens quinquefasciatus.";
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EnsemblMetazoa:CPIJ012618-PA}
RP   IDENTIFICATION.
RC   STRAIN=JHB {ECO:0000313|EnsemblMetazoa:CPIJ012618-PA};
RG   EnsemblMetazoa;
RL   Submitted (FEB-2021) to UniProtKB.
CC   -!- SIMILARITY: Belongs to the Ntn-hydrolase family.
CC       {ECO:0000256|ARBA:ARBA00010872}.
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DR   EMBL; DS232239; EDS38151.1; -; Genomic_DNA.
DR   RefSeq; XP_001863162.1; XM_001863127.1.
DR   AlphaFoldDB; B0X0J0; -.
DR   STRING; 7176.B0X0J0; -.
DR   EnsemblMetazoa; CPIJ012618-RA; CPIJ012618-PA; CPIJ012618.
DR   KEGG; cqu:CpipJ_CPIJ012618; -.
DR   VEuPathDB; VectorBase:CPIJ012618; -.
DR   VEuPathDB; VectorBase:CQUJHB004353; -.
DR   eggNOG; KOG1593; Eukaryota.
DR   HOGENOM; CLU_021603_0_0_1; -.
DR   InParanoid; B0X0J0; -.
DR   OMA; YKPIINI; -.
DR   Proteomes; UP000002320; Unassembled WGS sequence.
DR   GO; GO:0016787; F:hydrolase activity; IEA:InterPro.
DR   CDD; cd04513; Glycosylasparaginase; 1.
DR   Gene3D; 3.60.20.30; (Glycosyl)asparaginase; 1.
DR   InterPro; IPR029055; Ntn_hydrolases_N.
DR   InterPro; IPR000246; Peptidase_T2.
DR   PANTHER; PTHR10188; L-ASPARAGINASE; 1.
DR   PANTHER; PTHR10188:SF6; N(4)-(BETA-N-ACETYLGLUCOSAMINYL)-L-ASPARAGINASE; 1.
DR   Pfam; PF01112; Asparaginase_2; 1.
DR   SUPFAM; SSF56235; N-terminal nucleophile aminohydrolases (Ntn hydrolases); 1.
PE   3: Inferred from homology;
KW   Reference proteome {ECO:0000313|Proteomes:UP000002320};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           19..357
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5011409009"
FT   ACT_SITE        215
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR600246-1"
FT   BINDING         243..246
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR600246-2"
FT   BINDING         266..269
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR600246-2"
FT   SITE            214..215
FT                   /note="Cleavage; by autolysis"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR600246-3"
SQ   SEQUENCE   357 AA;  38301 MW;  25833473DFA6A127 CRC64;
     MASPRRILLL VAAILVQAHR SDQTLPLVIN TWAFTNATLR AHQWLVVGES GPIDALVEGC
     SVCEREQCDG TVGYGGSPDE NGETTLDAMI MDGSTMNIGA VAAMRDVKHA AAVARHVLEN
     TKHTLLVGDR ATEFAVQMGF KRESLETERS KEMWEEWKNN HCQPNFWQNV IPSPSMSCGP
     YEPISGNAIP QNHWTSNELN EDNHAPTFNR FNHDTIGMIV IDAEGHVVAG TSTNGARNKI
     PGRVGDSPIP GSGAYADSSV GAAAATGDGD IMMRFMPSLL AVEGLRRGLS PMQAGETALA
     RIAVHYPNFV GGIVVASSDG QYGAACHGMD EFPYSVAEGS KDTEVVVKKV KCRAKRG
//