ID B1YTC8_BURA4 Unreviewed; 364 AA.
AC B1YTC8;
DT 20-MAY-2008, integrated into UniProtKB/TrEMBL.
DT 20-MAY-2008, sequence version 1.
DT 29-MAY-2013, entry version 43.
DE RecName: Full=Dihydroorotase;
DE Short=DHOase;
DE EC=3.5.2.3;
GN Name=pyrC; OrderedLocusNames=BamMC406_0596;
OS Burkholderia ambifaria (strain MC40-6).
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
OX NCBI_TaxID=398577;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MC40-6;
RA Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E.,
RA Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L.,
RA Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA Lykidis A., Ramette A., Konstantinidis K., Tiedje J., Richardson P.;
RT "Complete sequence of chromosome 1 of Burkholderia ambifaria MC40-6.";
RL Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY: (S)-dihydroorotate + H(2)O = N-carbamoyl-L-
CC aspartate.
CC -!- COFACTOR: Binds 2 zinc ions per subunit (By similarity).
CC -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo
CC pathway; (S)-dihydroorotate from bicarbonate: step 3/3.
CC -!- SUBUNIT: Homodimer (By similarity).
CC -!- SIMILARITY: Belongs to the DHOase family. Type 1 subfamily.
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DR EMBL; CP001025; ACB63093.1; -; Genomic_DNA.
DR RefSeq; YP_001807309.1; NC_010551.1.
DR ProteinModelPortal; B1YTC8; -.
DR SMR; B1YTC8; 20-363.
DR STRING; 398577.BamMC406_0596; -.
DR EnsemblBacteria; ACB63093; ACB63093; BamMC406_0596.
DR GeneID; 6178756; -.
DR KEGG; bac:BamMC406_0596; -.
DR PATRIC; 19030504; VBIBurAmb82852_0601.
DR eggNOG; COG0418; -.
DR HOGENOM; HOG000256259; -.
DR KO; K01465; -.
DR OMA; MTLYLTE; -.
DR ProtClustDB; PRK05451; -.
DR BioCyc; BAMB398577:GH38-617-MONOMER; -.
DR UniPathway; UPA00070; UER00117.
DR GO; GO:0004151; F:dihydroorotase activity; IEA:HAMAP.
DR GO; GO:0008270; F:zinc ion binding; IEA:HAMAP.
DR GO; GO:0044205; P:'de novo' UMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0019856; P:pyrimidine nucleobase biosynthetic process; IEA:InterPro.
DR HAMAP; MF_00219; PyrC_type1; 1; -.
DR InterPro; IPR006680; Amidohydro_1.
DR InterPro; IPR004721; DHOdimr.
DR InterPro; IPR002195; Dihydroorotase_CS.
DR Pfam; PF01979; Amidohydro_1; 1.
DR PIRSF; PIRSF001237; DHOdimr; 1.
DR TIGRFAMs; TIGR00856; pyrC_dimer; 1.
DR PROSITE; PS00482; DIHYDROOROTASE_1; 1.
DR PROSITE; PS00483; DIHYDROOROTASE_2; 1.
PE 3: Inferred from homology;
KW Complete proteome; Hydrolase; Metal-binding; Pyrimidine biosynthesis;
KW Zinc.
FT METAL 31 31 Zinc 1 (By similarity).
FT METAL 33 33 Zinc 1 (By similarity).
FT METAL 117 117 Zinc 1; via carbamate group (By
FT similarity).
FT METAL 117 117 Zinc 2; via carbamate group (By
FT similarity).
FT METAL 154 154 Zinc 2 (By similarity).
FT METAL 192 192 Zinc 2 (By similarity).
FT METAL 268 268 Zinc 1 (By similarity).
FT MOD_RES 117 117 N6-carboxylysine (By similarity).
SQ SEQUENCE 364 AA; 39685 MW; EC4610ECCD74A4FA CRC64;
MASPFLTTPP MTASNASSPA TLTLARPDDL HLHLRDGDML AAVLPHTARQ FARAIVMPNL
KPPVTTTAHA QAYRERILAA LPAGMTFEPL MTLYLTDNTA PDEIRRARDS GFVHGVKLYP
AGATTNSDHG VSDLAKCAKT LEVMQETGMP LLVHGEVTDP SVDMFDREKV FIDRVMTPLR
RDFPGLKVVF EHITTKDAAD YVRDADAAPG LLGATITPQH MLYSRNALFV GGIRPHYYCL
PVLKRETHRV ALVEAATSGN PRFFLGTDSA PHARGAKETA CGCAGCYTAL HALELYAEAF
DQAGALDKLE NFASRFGADF YGLPHSTETV TLRRETWELP REVFAGDTPV VPLRAGEAIG
WKFA
//
