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Database: UniProt/TrEMBL
Entry: B2TQX0_CLOBB
LinkDB: B2TQX0_CLOBB
Original site: B2TQX0_CLOBB 
ID   B2TQX0_CLOBB            Unreviewed;       524 AA.
AC   B2TQX0; U4PHP6;
DT   01-JUL-2008, integrated into UniProtKB/TrEMBL.
DT   01-JUL-2008, sequence version 1.
DT   25-OCT-2017, entry version 62.
DE   SubName: Full=Alpha-amylase {ECO:0000313|EMBL:ACD24706.1};
DE            EC=3.2.1.1 {ECO:0000313|EMBL:ACD24706.1};
GN   OrderedLocusNames=CLL_A2370 {ECO:0000313|EMBL:ACD24706.1};
OS   Clostridium botulinum (strain Eklund 17B / Type B).
OC   Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=935198 {ECO:0000313|EMBL:ACD24706.1, ECO:0000313|Proteomes:UP000001195};
RN   [1] {ECO:0000313|Proteomes:UP000001195}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Eklund 17B / Type B {ECO:0000313|Proteomes:UP000001195};
RA   Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A.,
RA   Smith T.J., Sutton G., Brettin T.S.;
RT   "Complete sequence of Clostridium botulinum strain Eklund.";
RL   Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
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DR   EMBL; CP001056; ACD24706.1; -; Genomic_DNA.
DR   RefSeq; WP_012425441.1; NC_018648.1.
DR   ProteinModelPortal; B2TQX0; -.
DR   CAZy; GH13; Glycoside Hydrolase Family 13.
DR   EnsemblBacteria; ACD24706; ACD24706; CLL_A2370.
DR   GeneID; 19963364; -.
DR   KEGG; cbk:CLL_A2370; -.
DR   PATRIC; fig|935198.13.peg.2327; -.
DR   HOGENOM; HOG000094847; -.
DR   KO; K01176; -.
DR   OMA; VLNHKMG; -.
DR   Proteomes; UP000001195; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004556; F:alpha-amylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0103025; F:alpha-amylase activity (releasing maltohexaose); IEA:UniProtKB-EC.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   InterPro; IPR013776; A-amylase_thermo.
DR   InterPro; IPR015237; Alpha-amylase_C_pro.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   Pfam; PF09154; DUF1939; 1.
DR   PIRSF; PIRSF001021; Alph-amls_thrmst; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
PE   4: Predicted;
KW   Calcium {ECO:0000256|PIRSR:PIRSR001021-2};
KW   Complete proteome {ECO:0000313|Proteomes:UP000001195};
KW   Glycosidase {ECO:0000313|EMBL:ACD24706.1};
KW   Hydrolase {ECO:0000313|EMBL:ACD24706.1};
KW   Membrane {ECO:0000256|SAM:Phobius};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR001021-2};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001195};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM      6     23       Helical. {ECO:0000256|SAM:Phobius}.
FT   DOMAIN       48    445       Aamy. {ECO:0000259|SMART:SM00642}.
FT   ACT_SITE    275    275       Nucleophile. {ECO:0000256|PIRSR:
FT                                PIRSR001021-1}.
FT   ACT_SITE    305    305       Proton donor. {ECO:0000256|PIRSR:
FT                                PIRSR001021-1}.
FT   METAL       148    148       Calcium 1. {ECO:0000256|PIRSR:
FT                                PIRSR001021-2}.
FT   METAL       205    205       Calcium 2. {ECO:0000256|PIRSR:
FT                                PIRSR001021-2}.
FT   METAL       238    238       Calcium 1. {ECO:0000256|PIRSR:
FT                                PIRSR001021-2}.
FT   METAL       244    244       Calcium 1. {ECO:0000256|PIRSR:
FT                                PIRSR001021-2}.
FT   METAL       246    246       Calcium 2. {ECO:0000256|PIRSR:
FT                                PIRSR001021-2}.
FT   METAL       279    279       Calcium 1; via carbonyl oxygen.
FT                                {ECO:0000256|PIRSR:PIRSR001021-2}.
SQ   SEQUENCE   524 AA;  60666 MW;  84D0F00F02F5783E CRC64;
     MTVEIILIII VFAIIGGVMY LNLEQDKAKD VEKNEDKVYI EDKKIKNKTM MQYFEWYYPN
     DGSLWNKVKE HSKKLSKKGI TALWLPPAYK GVGGINDVGY GAYDLYDLGE FDQKGTIRTK
     YGTKEQYLDA INEAHNNNIE VYADTVFNHK AGADESECVK AQLVDSNNRN NIIGEEKEIR
     SFTVFNFSGR NDKYSSYKWN AKDFDGVDFD DLTKQNGIFK FVGKEWEQDV DDENGNFDFL
     MCADLDIDSR DVVEELKTWG KWYINECKLD GFRLDAIKHI KFDFFAEWLN DIRSKKGDNV
     FAVGEYWSGD INKLKYYISK SQNVMSLFDV PLHYKFFEAS NLEENFDMRT LTQNTLLDYD
     EKISVTFVDN HDTEPGQALE SWVKPWFKLI AYTFILTRQE GYPCIFYGDY YGVPEKGFEG
     FKNQLDMILN VRKDYAYGDQ CDYFDDKNII GWTRQGDLMH DNSGVAVLIS NGNNGSKKMS
     MGSKNISTTF IDITGNIQEE ILIDNEGNGI FKVNNKSYSI WIKK
//
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