ID   B3E0M6_METI4            Unreviewed;       670 AA.
AC   B3E0M6;
DT   22-JUL-2008, integrated into UniProtKB/TrEMBL.
DT   22-JUL-2008, sequence version 1.
DT   01-MAY-2013, entry version 39.
DE   SubName: Full=NAD synthetase and glutamine amidotransferase;
GN   Name=nadE; OrderedLocusNames=Minf_0725;
OS   Methylacidiphilum infernorum (isolate V4) (Methylokorus infernorum
OS   (strain V4)).
OC   Bacteria; Verrucomicrobia; unclassified Verrucomicrobia;
OC   Methylacidiphilales; Methylacidiphilaceae; Methylacidiphilum.
OX   NCBI_TaxID=481448;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Isolate V4;
RX   PubMed=18593465; DOI=10.1186/1745-6150-3-26;
RA   Hou S., Makarova K.S., Saw J.H., Senin P., Ly B.V., Zhou Z., Ren Y.,
RA   Wang J., Galperin M.Y., Omelchenko M.V., Wolf Y.I., Yutin N.,
RA   Koonin E.V., Stott M.B., Mountain B.W., Crowe M.A., Smirnova A.V.,
RA   Dunfield P.F., Feng L., Wang L., Alam M.;
RT   "Complete genome sequence of the extremely acidophilic methanotroph
RT   isolate V4, Methylacidiphilum infernorum, a representative of the
RT   bacterial phylum Verrucomicrobia.";
RL   Biol. Direct 3:26-26(2008).
CC   -!- SIMILARITY: Belongs to the NAD synthetase family.
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DR   EMBL; CP000975; ACD82780.1; -; Genomic_DNA.
DR   RefSeq; YP_001939378.1; NC_010794.1.
DR   ProteinModelPortal; B3E0M6; -.
DR   STRING; 481448.Minf_0725; -.
DR   EnsemblBacteria; ACD82780; ACD82780; Minf_0725.
DR   GeneID; 6351644; -.
DR   KEGG; min:Minf_0725; -.
DR   PATRIC; 22490517; VBIMetInf111569_0760.
DR   eggNOG; COG0388; -.
DR   HOGENOM; HOG000051304; -.
DR   KO; K01950; -.
DR   OMA; FLALHAW; -.
DR   ProtClustDB; PRK02628; -.
DR   BioCyc; MINF481448:GJEI-734-MONOMER; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016810; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds; IEA:InterPro.
DR   GO; GO:0003952; F:NAD+ synthase (glutamine-hydrolyzing) activity; IEA:InterPro.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0009435; P:NAD biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.40.50.620; -; 2.
DR   Gene3D; 3.60.110.10; -; 1.
DR   InterPro; IPR003010; C-N_Hydrolase.
DR   InterPro; IPR014445; Gln-dep_NAD_synthase.
DR   InterPro; IPR022310; NAD/GMP_synthase.
DR   InterPro; IPR003694; NAD_synthase.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   Pfam; PF00795; CN_hydrolase; 1.
DR   Pfam; PF02540; NAD_synthase; 1.
DR   PIRSF; PIRSF006630; NADS_GAT; 1.
DR   SUPFAM; SSF56317; Ntlse/CNhydtse; 1.
DR   TIGRFAMs; TIGR00552; nadE; 1.
DR   PROSITE; PS50263; CN_HYDROLASE; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Glutamine amidotransferase; Ligase;
KW   NAD; Nucleotide-binding; Transferase.
SQ   SEQUENCE   670 AA;  75546 MW;  828873CD76CE6A70 CRC64;
     MGKVADPFYN ATQIAELCRQ AADKKATLVV FPELCLSAYS CEDLFHQSAL LEVSLRALEY
     LLEETAPLSL LTLVGLPLRV NQLLYNCGCL FSRGKILGVI PKSYLPNYRE FYESRQFSQA
     ALATEEYVDL LGQKAIPFGT NLIFEWEQQP LFKLAIEICE DLWVPLPPSS FAALAGATVL
     VNLSASNITI GKSDYRKLLV ASQSGRCISA YIYSAAGFGE STTDLAWDGE GLIYENGTKL
     AETRRFAYDS QLVFADVDLD RLQADRMRQN SFGQTKVHFK KEISSFKTLT FSLDVNKESI
     LLLERVLERF PYVPSDPLTR DQRCQEVYAI QTQGLIQRLK ATSIRKTVIG ISGGLDSAHA
     LIVCAKAMDI LGFPRQNILA CTMPGFATTK KTLEQARRLI EATGCREYFI DIRPSCMQLF
     KDIGHPFARG EKLYDVTFEN VQAGERTNHL FRLANFENAL VVGTSDLSEL ALGWSTYGVG
     DHMAHYHVNA SVPKTLIKFL IRWVSKTQEL GEKVSEVLDE ILDTVISPEL IPGENAQEPL
     QSSEEQIGPY NLQDFNLYYT LRYGYLPTKT AFLCWSAWHD KNRGQWPEME KKREAYSIDQ
     IKYWLRVFLD RFFRTSQFKR SCIANAPKVG SGGSLSPRGD YRAPSDSSSA PWLKIWEEIP
     SSEDELLNKL
//