ID B6VKL9_PHOAA Unreviewed; 327 AA.
AC B6VKL9; C7BTD3;
DT 16-DEC-2008, integrated into UniProtKB/TrEMBL.
DT 16-DEC-2008, sequence version 1.
DT 01-MAY-2013, entry version 34.
DE RecName: Full=Succinylglutamate desuccinylase;
DE EC=3.5.1.96;
GN Name=astE; OrderedLocusNames=PAU_01501; ORFNames=PA-RVA3-3983;
OS Photorhabdus asymbiotica subsp. asymbiotica (strain ATCC 43949 /
OS 3105-77) (Xenorhabdus luminescens (strain 2)).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Photorhabdus.
OX NCBI_TaxID=553480;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 43949;
RX PubMed=18838673; DOI=10.1073/pnas.0711114105;
RA Waterfield N.R., Sanchez-Contreras M., Eleftherianos I., Dowling A.,
RA Yang G., Wilkinson P., Parkhill J., Thomson N., Reynolds S.E.,
RA Bode H.B., Dorus S., Ffrench-Constant R.H.;
RT "Rapid virulence annotation (RVA): identification of virulence factors
RT using a bacterial genome library and multiple invertebrate hosts.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:15967-15972(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC43949;
RA Crossman L.C.;
RL Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 43949;
RA Thomson N.R.;
RL Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43949 / 3105-77, and ATCC43949;
RX PubMed=19583835; DOI=10.1186/1471-2164-10-302;
RA Wilkinson P., Waterfield N.R., Crossman L., Corton C.,
RA Sanchez-Contreras M., Vlisidou I., Barron A., Bignell A., Clark L.,
RA Doggett J., Ormond D., Mayho M., Bason N., Smith F., Simmonds M.,
RA Arrowsmith C., Churcher C., Harris D., Thompson N.R., Quail M.,
RA Parkhill J., Ffrench-Constant R.H.;
RT "Comparative genomics of the emerging human pathogen Photorhabdus
RT asymbiotica with the insect pathogen Photorhabdus luminescens.";
RL BMC Genomics 10:302-302(2009).
CC -!- FUNCTION: Transforms N(2)-succinylglutamate into succinate and
CC glutamate (By similarity).
CC -!- CATALYTIC ACTIVITY: N-succinyl-L-glutamate + H(2)O = succinate +
CC L-glutamate.
CC -!- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
CC -!- PATHWAY: Amino-acid degradation; L-arginine degradation via AST
CC pathway; L-glutamate and succinate from L-arginine: step 5/5.
CC -!- SIMILARITY: Belongs to the AspA/AstE family. Succinylglutamate
CC desuccinylase subfamily.
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DR EMBL; FM162591; CAQ83593.1; -; Genomic_DNA.
DR EMBL; FM211045; CAR66699.1; -; Genomic_DNA.
DR RefSeq; YP_003040338.1; NC_012962.1.
DR STRING; 291112.PAU_01501; -.
DR EnsemblBacteria; CAQ83593; CAQ83593; PAU_01501.
DR GeneID; 13861068; -.
DR KEGG; pay:PAU_01501; -.
DR PATRIC; 20496814; VBIPhoAsy71438_1678.
DR HOGENOM; HOG000280300; -.
DR KO; K05526; -.
DR OMA; YLHNDLN; -.
DR ProtClustDB; PRK05324; -.
DR BioCyc; PASY291112:GJQ0-1564-MONOMER; -.
DR UniPathway; UPA00185; UER00283.
DR GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:HAMAP.
DR GO; GO:0009017; F:succinylglutamate desuccinylase activity; IEA:EC.
DR GO; GO:0008270; F:zinc ion binding; IEA:HAMAP.
DR GO; GO:0019544; P:arginine catabolic process to glutamate; IEA:HAMAP.
DR GO; GO:0019545; P:arginine catabolic process to succinate; IEA:UniProtKB-UniPathway.
DR HAMAP; MF_00767; Arg_catab_AstE; 1; -.
DR InterPro; IPR007036; Aste_AspA.
DR InterPro; IPR016681; SuccinylGlu_desuccinylase.
DR Pfam; PF04952; AstE_AspA; 1.
DR TIGRFAMs; TIGR03242; arg_catab_astE; 1.
PE 3: Inferred from homology;
KW Arginine metabolism; Complete proteome; Hydrolase; Metal-binding;
KW Zinc.
FT ACT_SITE 211 211 By similarity.
FT METAL 51 51 Zinc (By similarity).
FT METAL 54 54 Zinc (By similarity).
FT METAL 148 148 Zinc (By similarity).
SQ SEQUENCE 327 AA; 36879 MW; 3A966092CBC83CFF CRC64;
MDLLTLLLEK KLADHLRFPE TISASWLAEG VLQLIPDKRK NRSLVVSVGI HGNETAPIEI
LIQLLAQLAD GTLTLENNLL IIFGNLPAIR ANRRYLHNDL NRMFGGRYLN FPLGNERSRA
AELENVVSRF FAEPSVSATN IRWHIDLHTA IRASHHEQFA LLPAQGRPFS AEFMQWLSDS
NIEALVYHKE KGGTFSHFLG EKFGTDSCTM EIGKVMPFGK NDLKRFQKVI EALHGLIDHS
QITARTKPEL KHYQVVDSII KSDDSFQLHI PADTMNFTEL PAGFEIASQR DRHWKITSSV
NFILFPNAEV ANGLRAGLLL ALNKKNN
//
