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Database: UniProt/TrEMBL
Entry: B7NEM4_ECOLU
LinkDB: B7NEM4_ECOLU
Original site: B7NEM4_ECOLU 
ID   B7NEM4_ECOLU            Unreviewed;       676 AA.
AC   B7NEM4;
DT   10-FEB-2009, integrated into UniProtKB/TrEMBL.
DT   10-FEB-2009, sequence version 1.
DT   27-SEP-2017, entry version 53.
DE   SubName: Full=Alpha-amylase {ECO:0000313|EMBL:CAR15225.1};
DE            EC=3.2.1.1 {ECO:0000313|EMBL:CAR15225.1};
GN   Name=malS {ECO:0000313|EMBL:CAR15225.1};
GN   OrderedLocusNames=ECUMN_4084 {ECO:0000313|EMBL:CAR15225.1};
OS   Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=585056 {ECO:0000313|EMBL:CAR15225.1, ECO:0000313|Proteomes:UP000007097};
RN   [1] {ECO:0000313|Proteomes:UP000007097}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UMN026 / ExPEC {ECO:0000313|Proteomes:UP000007097};
RX   PubMed=19165319; DOI=10.1371/journal.pgen.1000344;
RA   Touchon M., Hoede C., Tenaillon O., Barbe V., Baeriswyl S., Bidet P.,
RA   Bingen E., Bonacorsi S., Bouchier C., Bouvet O., Calteau A.,
RA   Chiapello H., Clermont O., Cruveiller S., Danchin A., Diard M.,
RA   Dossat C., Karoui M.E., Frapy E., Garry L., Ghigo J.M., Gilles A.M.,
RA   Johnson J., Le Bouguenec C., Lescat M., Mangenot S.,
RA   Martinez-Jehanne V., Matic I., Nassif X., Oztas S., Petit M.A.,
RA   Pichon C., Rouy Z., Ruf C.S., Schneider D., Tourret J., Vacherie B.,
RA   Vallenet D., Medigue C., Rocha E.P.C., Denamur E.;
RT   "Organised genome dynamics in the Escherichia coli species results in
RT   highly diverse adaptive paths.";
RL   PLoS Genet. 5:E1000344-E1000344(2009).
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DR   EMBL; CU928163; CAR15225.1; -; Genomic_DNA.
DR   RefSeq; WP_000761238.1; NC_011751.1.
DR   RefSeq; YP_002414725.1; NC_011751.1.
DR   ProteinModelPortal; B7NEM4; -.
DR   CAZy; GH13; Glycoside Hydrolase Family 13.
DR   EnsemblBacteria; CAR15225; CAR15225; ECUMN_4084.
DR   GeneID; 7155854; -.
DR   KEGG; eum:ECUMN_4084; -.
DR   PATRIC; fig|585056.7.peg.4258; -.
DR   HOGENOM; HOG000273912; -.
DR   KO; K01176; -.
DR   OMA; DKVMVVW; -.
DR   Proteomes; UP000007097; Chromosome.
DR   GO; GO:0042597; C:periplasmic space; IEA:InterPro.
DR   GO; GO:0004556; F:alpha-amylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0103025; F:alpha-amylase activity (releasing maltohexaose); IEA:UniProtKB-EC.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   GO; GO:0030980; P:alpha-glucan catabolic process; IEA:InterPro.
DR   GO; GO:0051692; P:cellular oligosaccharide catabolic process; IEA:InterPro.
DR   InterPro; IPR014635; A_amylase_MalS.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   PIRSF; PIRSF036917; Alph_amls_MalS; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; SSF51445; 2.
PE   4: Predicted;
KW   Complete proteome {ECO:0000313|Proteomes:UP000007097};
KW   Glycosidase {ECO:0000313|EMBL:CAR15225.1};
KW   Hydrolase {ECO:0000313|EMBL:CAR15225.1};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL        1     17       {ECO:0000256|SAM:SignalP}.
FT   CHAIN        18    676       {ECO:0000256|SAM:SignalP}.
FT                                /FTId=PRO_5002861181.
FT   DOMAIN      193    637       Aamy. {ECO:0000259|SMART:SM00642}.
SQ   SEQUENCE   676 AA;  75705 MW;  AC942EBE3BAA7BDD CRC64;
     MKLAACFLTL LPGFAVAASW TSPGFPAFSE QGTGTFVSHA QLPKGTRPLT LNFDQQCWQP
     ADAIKLNQML SLQPCSNTPP QWRLFRDGEY TLQLDTRSGT PTLMISIQNA VEPVASLVRE
     CPIWDGLPLT LDVSATFPEG AAVRDYYSQQ IAIVKNGQIT LQPAATSNGL LLLERAETDA
     PAPFDWHNAT VYFVLTDRFE NGDPSNDQSY GRHKDGMAEI GTFHGGDLRG LTNKLDYLQQ
     LGVNALWISA PFEQIHGWVG GGTKGDFPHY AYHGYYTQDW TNLDANMGSE ADLRTLVDSA
     HQRGIRILFD VVMNHTGYAT LADMQEYQFG ALYLSGDEVK KTLGERWSDW KPAAGQTWHS
     FNDYINFSDK TGWDKWWGKN WIRTDIGDYD NPGFDDLTMS LAFLPDIKTE STTASGLPVF
     YKNKTDTHAK VIDGFTPRDY LTHWLSQWVR DYGIDGFRVD TAKHVELPAW QQLKTEASAA
     LREWKKANPD KALDDKPFWM TGEAWGHGVM QSDYYRHGFD AMINFDYQEQ AAKAVDCLAQ
     MDTTWQQMAE KLQDFNVLSY LSSHDTRLFR EGGDKAAELL LLAPGAVQIF YGDESSRPFG
     PTGSDPLQGT RSDMNWQDVS GKSAASVAHW QKISQFRARH PAIGAGKQTT LSLKQGYGFV
     REHGDDKVLV IWAGQQ
//
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