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Database: UniProt/TrEMBL
Entry: B8DG55_LISMH
LinkDB: B8DG55_LISMH
Original site: B8DG55_LISMH 
ID   B8DG55_LISMH            Unreviewed;       434 AA.
AC   B8DG55;
DT   03-MAR-2009, integrated into UniProtKB/TrEMBL.
DT   03-MAR-2009, sequence version 1.
DT   11-JUN-2014, entry version 37.
DE   RecName: Full=Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO;
DE            EC=2.1.1.74;
DE   AltName: Full=Folate-dependent tRNA (uracil-5-)-methyltransferase;
DE   AltName: Full=Folate-dependent tRNA(M-5-U54)-methyltransferase;
GN   Name=trmFO; OrderedLocusNames=LMHCC_1300;
OS   Listeria monocytogenes serotype 4a (strain HCC23).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria.
OX   NCBI_TaxID=552536;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HCC23;
RX   PubMed=21602330; DOI=10.1128/JB.05236-11;
RA   Steele C.L., Donaldson J.R., Paul D., Banes M.M., Arick T.,
RA   Bridges S.M., Lawrence M.L.;
RT   "Genome sequence of lineage III Listeria monocytogenes strain HCC23.";
RL   J. Bacteriol. 193:3679-3680(2011).
CC   -!- FUNCTION: Catalyzes the folate-dependent formation of 5-methyl-
CC       uridine at position 54 (M-5-U54) in all tRNAs (By similarity).
CC   -!- CATALYTIC ACTIVITY: 5,10-methylenetetrahydrofolate + uracil(54) in
CC       tRNA + FADH(2) = tetrahydrofolate + 5-methyluracil(54) in tRNA +
CC       FAD.
CC   -!- COFACTOR: FAD (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the MnmG family. TrmFO subfamily.
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DR   EMBL; CP001175; ACK39645.1; -; Genomic_DNA.
DR   RefSeq; YP_002350259.1; NC_011660.1.
DR   ProteinModelPortal; B8DG55; -.
DR   STRING; 552536.LMHCC_1300; -.
DR   EnsemblBacteria; ACK39645; ACK39645; LMHCC_1300.
DR   GeneID; 7080230; -.
DR   KEGG; lmh:LMHCC_1300; -.
DR   PATRIC; 20317713; VBILisMon86872_1287.
DR   eggNOG; COG1206; -.
DR   HOGENOM; HOG000252054; -.
DR   KO; K04094; -.
DR   OMA; EIYFEGC; -.
DR   OrthoDB; EOG6J74VT; -.
DR   BioCyc; LMON552536:GIW4-1333-MONOMER; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0030698; F:5,10-methylenetetrahydrofolate-dependent tRNA (m5U54) methyltransferase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0047151; F:methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity; IEA:UniProtKB-EC.
DR   HAMAP; MF_01037; TrmFO; 1.
DR   InterPro; IPR004417; Folate-dep_Ribothymidyl_synth.
DR   InterPro; IPR002218; GIDA-rel.
DR   InterPro; IPR020595; GIDA-rel_CS.
DR   Pfam; PF01134; GIDA; 1.
DR   TIGRFAMs; TIGR00137; gid_trmFO; 1.
DR   PROSITE; PS01281; GIDA_2; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; FAD; Flavoprotein; Methyltransferase;
KW   Transferase; tRNA processing.
FT   NP_BIND       9     14       FAD (By similarity){EA2}.
SQ   SEQUENCE   434 AA;  47851 MW;  DAA870400DF43EAA CRC64;
     MEKSVNVIGA GLAGSEAAWQ LVKRGVKVDL YEMRPVKQTP AHHTDKFAEL VCTNSLRANG
     LTNAVGVIKE EMRILDSIII EAADKASVPA GGALAVDRHE FSGYITDKVK NHPLVTVHTE
     EVTTIPEGPT IIATGPLTSP ALADEIKQLT GEEYLYFYDA AAPIIEKDSI DMDKVYLKSR
     YDKGEAAYLN CPMSEEEFNA FYEALVTAET AALKEFEKEV FFEGCMPIEV MAKRGIKTML
     FGPLKPVGLE DPKTGKRPYA VLQLRQDDAA GTLYNMVGFQ THLKWGEQKR VFGMIPGLEN
     AEIVRYGVMH RNTFINSPTV LEPTYQLKTR NDLFFAGQMT GVEGYVESAA SGLAAGINAA
     NFVEEKELVV FPAETAIGSL AHYITSASKK SFQPMNVNFG LFPELETKIR AKQERNEKLA
     ERALNAIKRV AEQL
//
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