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Database: UniProt/TrEMBL
Entry: B8EIA1_METSB
LinkDB: B8EIA1_METSB
Original site: B8EIA1_METSB 
ID   B8EIA1_METSB            Unreviewed;       366 AA.
AC   B8EIA1;
DT   03-MAR-2009, integrated into UniProtKB/TrEMBL.
DT   03-MAR-2009, sequence version 1.
DT   22-JUL-2015, entry version 46.
DE   RecName: Full=N5-carboxyaminoimidazole ribonucleotide synthase {ECO:0000256|HAMAP-Rule:MF_01928, ECO:0000256|RuleBase:RU361200};
DE            Short=N5-CAIR synthase {ECO:0000256|HAMAP-Rule:MF_01928, ECO:0000256|RuleBase:RU361200};
DE            EC=6.3.4.18 {ECO:0000256|HAMAP-Rule:MF_01928, ECO:0000256|RuleBase:RU361200};
DE   AltName: Full=5-(carboxyamino)imidazole ribonucleotide synthetase {ECO:0000256|HAMAP-Rule:MF_01928, ECO:0000256|RuleBase:RU361200};
GN   Name=purK {ECO:0000256|HAMAP-Rule:MF_01928,
GN   ECO:0000256|RuleBase:RU361200};
GN   OrderedLocusNames=Msil_2288 {ECO:0000313|EMBL:ACK51220.1};
OS   Methylocella silvestris (strain BL2 / DSM 15510 / NCIMB 13906).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Beijerinckiaceae; Methylocella.
OX   NCBI_TaxID=395965 {ECO:0000313|EMBL:ACK51220.1, ECO:0000313|Proteomes:UP000002257};
RN   [1] {ECO:0000313|EMBL:ACK51220.1, ECO:0000313|Proteomes:UP000002257}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=BL2 / DSM 15510 / NCIMB 13906
RC   {ECO:0000313|Proteomes:UP000002257};
RX   PubMed=20472789; DOI=10.1128/JB.00506-10;
RA   Chen Y., Crombie A., Rahman M.T., Dedysh S.N., Liesack W., Stott M.B.,
RA   Alam M., Theisen A.R., Murrell J.C., Dunfield P.F.;
RT   "Complete genome sequence of the aerobic facultative methanotroph
RT   Methylocella silvestris BL2.";
RL   J. Bacteriol. 192:3840-3841(2010).
CC   -!- FUNCTION: Catalyzes the ATP-dependent conversion of 5-
CC       aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5-
CC       carboxyaminoimidazole ribonucleotide (N5-CAIR).
CC       {ECO:0000256|HAMAP-Rule:MF_01928}.
CC   -!- FUNCTION: Catalyzes the ATP-dependent conversion of 5-
CC       aminoimidazole ribonucleotide (AIR) and HCO(3)- to N5-
CC       carboxyaminoimidazole ribonucleotide (N5-CAIR).
CC       {ECO:0000256|RuleBase:RU361200}.
CC   -!- CATALYTIC ACTIVITY: ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole
CC       + HCO(3)(-) = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-
CC       ribosyl)imidazole. {ECO:0000256|HAMAP-Rule:MF_01928,
CC       ECO:0000256|RuleBase:RU361200}.
CC   -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway;
CC       5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-
CC       amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 1/2.
CC       {ECO:0000256|HAMAP-Rule:MF_01928, ECO:0000256|RuleBase:RU361200}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_01928,
CC       ECO:0000256|RuleBase:RU361200}.
CC   -!- SIMILARITY: Belongs to the PurK/PurT family. {ECO:0000256|HAMAP-
CC       Rule:MF_01928, ECO:0000256|RuleBase:RU361200}.
CC   -!- SIMILARITY: Contains 1 ATP-grasp domain. {ECO:0000256|HAMAP-
CC       Rule:MF_01928}.
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DR   EMBL; CP001280; ACK51220.1; -; Genomic_DNA.
DR   RefSeq; WP_012591289.1; NC_011666.1.
DR   ProteinModelPortal; B8EIA1; -.
DR   STRING; 395965.Msil_2288; -.
DR   EnsemblBacteria; ACK51220; ACK51220; Msil_2288.
DR   KEGG; msl:Msil_2288; -.
DR   PATRIC; 22600227; VBIMetSil55537_2473.
DR   eggNOG; COG0026; -.
DR   HOGENOM; HOG000034029; -.
DR   KO; K01589; -.
DR   OMA; LRKMGHV; -.
DR   OrthoDB; EOG6QZMX7; -.
DR   BioCyc; MSIL395965:GCND-2332-MONOMER; -.
DR   UniPathway; UPA00074; UER00942.
DR   Proteomes; UP000002257; Chromosome.
DR   GO; GO:0034028; F:5-(carboxyamino)imidazole ribonucleotide synthase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0004638; F:phosphoribosylaminoimidazole carboxylase activity; IEA:InterPro.
DR   GO; GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-HAMAP.
DR   Gene3D; 3.30.1490.20; -; 1.
DR   Gene3D; 3.30.470.20; -; 1.
DR   Gene3D; 3.40.50.20; -; 1.
DR   HAMAP; MF_01928; PurK; 1.
DR   InterPro; IPR011761; ATP-grasp.
DR   InterPro; IPR003135; ATP-grasp_carboxylate-amine.
DR   InterPro; IPR013815; ATP_grasp_subdomain_1.
DR   InterPro; IPR013816; ATP_grasp_subdomain_2.
DR   InterPro; IPR016185; PreATP-grasp_dom.
DR   InterPro; IPR005875; PurK.
DR   InterPro; IPR011054; Rudment_hybrid_motif.
DR   Pfam; PF02222; ATP-grasp; 1.
DR   SUPFAM; SSF51246; SSF51246; 1.
DR   SUPFAM; SSF52440; SSF52440; 1.
DR   TIGRFAMs; TIGR01161; purK; 1.
DR   PROSITE; PS50975; ATP_GRASP; 1.
PE   3: Inferred from homology;
KW   ATP-binding {ECO:0000256|HAMAP-Rule:MF_01928,
KW   ECO:0000256|RuleBase:RU361200};
KW   Complete proteome {ECO:0000313|Proteomes:UP000002257};
KW   Ligase {ECO:0000256|HAMAP-Rule:MF_01928,
KW   ECO:0000256|RuleBase:RU361200}; Lyase {ECO:0000313|EMBL:ACK51220.1};
KW   Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_01928,
KW   ECO:0000256|RuleBase:RU361200};
KW   Purine biosynthesis {ECO:0000256|HAMAP-Rule:MF_01928,
KW   ECO:0000256|RuleBase:RU361200};
KW   Reference proteome {ECO:0000313|Proteomes:UP000002257}.
FT   DOMAIN      116    306       ATP-grasp. {ECO:0000256|HAMAP-Rule:
FT                                MF_01928}.
FT   NP_BIND     157    163       ATP. {ECO:0000256|HAMAP-Rule:MF_01928}.
FT   NP_BIND     276    277       ATP. {ECO:0000256|HAMAP-Rule:MF_01928}.
FT   BINDING     112    112       ATP. {ECO:0000256|HAMAP-Rule:MF_01928}.
FT   BINDING     152    152       ATP. {ECO:0000256|HAMAP-Rule:MF_01928}.
FT   BINDING     195    195       ATP. {ECO:0000256|HAMAP-Rule:MF_01928}.
FT   BINDING     218    218       ATP. {ECO:0000256|HAMAP-Rule:MF_01928}.
SQ   SEQUENCE   366 AA;  38940 MW;  23F14727C8DB714B CRC64;
     MAFTLSPGAL APGSRIGILG GGQLGRMLAM AAARLGLHAH IYAPEPDSTA FEVCRERTLA
     AYEDEEALAR FAESVDAVTY EFENVPARTA ALLAGLRPVR PSAAALAVCQ DRLIEKEFLA
     DIGVATVNFM QVDHAGAMAR AVAQLGRPSI LKTRRFGYDG KGQVLVREGA DLAVTFRSLG
     GGPAILEAVA PFTKEISVVA ARGASGEFAA FDVCENTHEN HILKFTTAPA RIAVQTAAEA
     VLLTRAIAEA LDYVGVLAVE MFVIEPAGGG EQLLVNEIAP RVHNSGHWTL DGAATSQFEQ
     HIRAIAGWPL GATFLNGSEV EMENLIGEDI YAFEAILREP GACLHLYGKA EARAGRKMGH
     VTRIRR
//
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