ID B9M1E5_GEOSF Unreviewed; 400 AA.
AC B9M1E5;
DT 24-MAR-2009, integrated into UniProtKB/TrEMBL.
DT 24-MAR-2009, sequence version 1.
DT 01-MAY-2013, entry version 36.
DE RecName: Full=Riboflavin biosynthesis protein RibBA;
GN Name=ribBA; OrderedLocusNames=Geob_2677;
OS Geobacter sp. (strain FRC-32).
OC Bacteria; Proteobacteria; Deltaproteobacteria; Desulfuromonadales;
OC Geobacteraceae; Geobacter.
OX NCBI_TaxID=316067;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FRC-32;
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E.,
RA Tice H., Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T.,
RA Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N.,
RA Ovchinnikova G., Kostka J., Richardson P.;
RT "Complete sequence of Geobacter sp. FRC-32.";
RL Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the conversion of D-ribulose 5-phosphate to
CC formate and 3,4-dihydroxy-2-butanone 4-phosphate (By similarity).
CC -!- FUNCTION: Catalyzes the conversion of GTP to 2,5-diamino-6-
CC ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and
CC pyrophosphate (By similarity).
CC -!- CATALYTIC ACTIVITY: D-ribulose 5-phosphate = formate + L-3,4-
CC dihydroxybutan-2-one 4-phosphate.
CC -!- CATALYTIC ACTIVITY: GTP + 3 H(2)O = formate + 2,5-diamino-6-
CC hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate.
CC -!- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
CC -!- COFACTOR: Binds 2 divalent metal cations per subunit. Magnesium or
CC manganese (By similarity).
CC -!- PATHWAY: Cofactor biosynthesis; riboflavin biosynthesis; 2-
CC hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step
CC 1/1.
CC -!- PATHWAY: Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-
CC 6-(D-ribitylamino)uracil from GTP: step 1/4.
CC -!- SIMILARITY: In the C-terminal section; belongs to the GTP
CC cyclohydrolase II family.
CC -!- SIMILARITY: In the N-terminal section; belongs to the DHBP
CC synthase family.
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DR EMBL; CP001390; ACM21027.1; -; Genomic_DNA.
DR RefSeq; YP_002538128.1; NC_011979.1.
DR ProteinModelPortal; B9M1E5; -.
DR SMR; B9M1E5; 3-203, 206-376.
DR STRING; 316067.Geob_2677; -.
DR EnsemblBacteria; ACM21027; ACM21027; Geob_2677.
DR GeneID; 7361332; -.
DR KEGG; geo:Geob_2677; -.
DR PATRIC; 22012021; VBIGeoSp137169_2768.
DR eggNOG; COG0108; -.
DR HOGENOM; HOG000115440; -.
DR KO; K14652; -.
DR OMA; LRCDCRM; -.
DR ProtClustDB; CLSK828487; -.
DR BioCyc; GSP316067:GHSV-2708-MONOMER; -.
DR UniPathway; UPA00275; UER00399.
DR GO; GO:0008686; F:3,4-dihydroxy-2-butanone-4-phosphate synthase activity; IEA:InterPro.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003935; F:GTP cyclohydrolase II activity; IEA:EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009231; P:riboflavin biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.90.870.10; -; 1.
DR HAMAP; MF_00179; RibA; 1; -.
DR HAMAP; MF_00180; RibB; 1; -.
DR HAMAP; MF_01283; RibBA; 1; -.
DR InterPro; IPR017945; DHBP_synth_RibB-like_a/b_dom.
DR InterPro; IPR000422; DHBP_synthase_RibB.
DR InterPro; IPR000926; GTP_CycHdrlaseII_RibA.
DR InterPro; IPR016299; Riboflavin_synth_RibBA.
DR Pfam; PF00926; DHBP_synthase; 1.
DR Pfam; PF00925; GTP_cyclohydro2; 1.
DR PIRSF; PIRSF001259; RibA; 1.
DR SUPFAM; SSF55821; DHBP_synth_RibB-like_a/b_dom; 1.
DR TIGRFAMs; TIGR00505; ribA; 1.
DR TIGRFAMs; TIGR00506; ribB; 1.
PE 3: Inferred from homology;
KW Complete proteome; GTP-binding; Hydrolase; Lyase; Magnesium;
KW Manganese; Metal-binding; Multifunctional enzyme; Nucleotide-binding;
KW Riboflavin biosynthesis; Zinc.
FT NP_BIND 252 256 GTP (By similarity).
FT NP_BIND 295 297 GTP (By similarity).
FT REGION 1 201 DHBP synthase (By similarity).
FT REGION 27 28 D-ribulose 5-phosphate binding (By
FT similarity).
FT REGION 202 400 GTP cyclohydrolase II (By similarity).
FT ACT_SITE 329 329 Proton acceptor; for GTP cyclohydrolase
FT activity (By similarity).
FT ACT_SITE 331 331 Nucleophile; for GTP cyclohydrolase
FT activity (By similarity).
FT METAL 28 28 Magnesium or manganese 1 (By similarity).
FT METAL 28 28 Magnesium or manganese 2 (By similarity).
FT METAL 257 257 Zinc; catalytic (By similarity).
FT METAL 268 268 Zinc; catalytic (By similarity).
FT METAL 270 270 Zinc; catalytic (By similarity).
FT BINDING 32 32 D-ribulose 5-phosphate (By similarity).
FT BINDING 164 164 D-ribulose 5-phosphate (By similarity).
FT BINDING 273 273 GTP (By similarity).
FT BINDING 317 317 GTP (By similarity).
FT BINDING 352 352 GTP (By similarity).
FT BINDING 357 357 GTP (By similarity).
FT SITE 126 126 Essential for DHBP synthase activity (By
FT similarity).
FT SITE 164 164 Essential for DHBP synthase activity (By
FT similarity).
SQ SEQUENCE 400 AA; 43536 MW; 78FDC869BFE9DB04 CRC64;
MTVAKIEDAI EDIRQGKMVI LVDDEDRENE GDLTMAAEKV TPEAINFMAK YGRGLICLTL
TGDKCDQLNL PPMVSTNTSS FGTAFTVSIE AKKGVTTGIS AADRAHTILT AVADDASADD
LARPGHVFPL RAKAGGVLVR AGQTEGSVDL ARLAGLKHAG VICEIMNDDG TMSRMPELKK
FAKEHGIKVC TIADLVAYRL KHESLVRRAA EVRLPSQLGE FSAIAFENDI DKLEHLALVK
GEIRGDEPVL VRVHSECLTG DVFASIRCDC AEQLHRAMEM VEAEGKGVVL YMRQEGRGIG
LINKLKAYAL QDQGKDTVEA NLALGFKADL RDYGIGAQIL VNLGIKNIRL MTNNPKKIVG
LEGYGINIVE RVPIEIAPSM SNKKYLKTKR EKMGHLLENI
//
