ID C0R088_BRAHW Unreviewed; 415 AA.
AC C0R088;
DT 05-MAY-2009, integrated into UniProtKB/TrEMBL.
DT 05-MAY-2009, sequence version 1.
DT 01-MAY-2013, entry version 32.
DE RecName: Full=3-isopropylmalate dehydratase large subunit;
DE EC=4.2.1.33;
DE AltName: Full=Alpha-IPM isomerase;
DE AltName: Full=Isopropylmalate isomerase;
GN Name=leuC; OrderedLocusNames=BHWA1_01043;
OS Brachyspira hyodysenteriae (strain ATCC 49526 / WA1).
OC Bacteria; Spirochaetes; Spirochaetales; Brachyspiraceae; Brachyspira.
OX NCBI_TaxID=565034;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 49526 / WA1;
RX PubMed=19262690; DOI=10.1371/journal.pone.0004641;
RA Bellgard M.I., Wanchanthuek P., La T., Ryan K., Moolhuijzen P.,
RA Albertyn Z., Shaban B., Motro Y., Dunn D.S., Schibeci D., Hunter A.,
RA Barrero R., Phillips N.D., Hampson D.J.;
RT "Genome sequence of the pathogenic intestinal spirochete brachyspira
RT hyodysenteriae reveals adaptations to its lifestyle in the porcine
RT large intestine.";
RL PLoS ONE 4:e4641-e4641(2009).
CC -!- FUNCTION: Catalyzes the isomerization between 2-isopropylmalate
CC and 3-isopropylmalate, via the formation of 2-isopropylmaleate (By
CC similarity).
CC -!- CATALYTIC ACTIVITY: (2R,3S)-3-isopropylmalate = (2S)-2-
CC isopropylmalate.
CC -!- COFACTOR: Binds 1 4Fe-4S cluster per subunit (By similarity).
CC -!- PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-
CC leucine from 3-methyl-2-oxobutanoate: step 2/4.
CC -!- SUBUNIT: Heterodimer of LeuC and LeuD (By similarity).
CC -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family. LeuC
CC type 2 subfamily.
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DR EMBL; CP001357; ACN83526.1; -; Genomic_DNA.
DR RefSeq; YP_002721230.1; NC_012225.1.
DR STRING; 565034.BHWA1_01043; -.
DR EnsemblBacteria; ACN83526; ACN83526; BHWA1_01043.
DR GeneID; 7666144; -.
DR KEGG; bhy:BHWA1_01043; -.
DR PATRIC; 21180173; VBIBraHyo62857_1024.
DR eggNOG; COG0065; -.
DR HOGENOM; HOG000226971; -.
DR KO; K01703; -.
DR OMA; VIPFDHQ; -.
DR ProtClustDB; PRK00402; -.
DR BioCyc; BHYO565034:GJI7-1038-MONOMER; -.
DR UniPathway; UPA00048; UER00071.
DR GO; GO:0003861; F:3-isopropylmalate dehydratase activity; IEA:HAMAP.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009098; P:leucine biosynthetic process; IEA:HAMAP.
DR Gene3D; 3.30.499.10; -; 2.
DR Gene3D; 3.40.1060.10; -; 1.
DR HAMAP; MF_01027; LeuC_type2; 1; -.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR015937; Acoase/IPM_deHydtase.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015932; Aconitase/IPMdHydase_lsu_aba_2.
DR InterPro; IPR018136; Aconitase_4Fe-4S_BS.
DR InterPro; IPR011826; HAcnase/IPMdehydase_lsu_prok.
DR InterPro; IPR006251; Homoacnase/IPMdehydase_lsu.
DR InterPro; IPR011823; IsopropMal_deHydtase_lsu_bac.
DR PANTHER; PTHR11670; PTHR11670; 1.
DR PANTHER; PTHR11670:SF6; PTHR11670:SF6; 1.
DR Pfam; PF00330; Aconitase; 2.
DR PRINTS; PR00415; ACONITASE.
DR SUPFAM; SSF53732; Aconitase_N; 1.
DR TIGRFAMs; TIGR01343; hacA_fam; 1.
DR TIGRFAMs; TIGR02086; IPMI_arch; 1.
DR TIGRFAMs; TIGR02083; LEU2; 1.
DR PROSITE; PS00450; ACONITASE_1; 1.
DR PROSITE; PS01244; ACONITASE_2; 1.
PE 3: Inferred from homology;
KW 4Fe-4S; Amino-acid biosynthesis;
KW Branched-chain amino acid biosynthesis; Complete proteome; Iron;
KW Iron-sulfur; Leucine biosynthesis; Lyase; Metal-binding.
FT METAL 298 298 Iron-sulfur (4Fe-4S) (By similarity).
FT METAL 358 358 Iron-sulfur (4Fe-4S) (By similarity).
FT METAL 361 361 Iron-sulfur (4Fe-4S) (By similarity).
SQ SEQUENCE 415 AA; 44758 MW; AE522FB7B34D20CB CRC64;
MTITQKILAA HAGVEKVEAG ELIMVKTDLV LGNDITSPVA INEFEKYGFD KVFDKEKIAL
VMDHFAPNKD IKAAEQCKQC RDFANKYDIS NYYDVGDMGV EHALLPEKGL VAPGEVIIGA
DSHTCTYGAF GAFSTGVGST DMAAAMATGQ VWFKVPSAIK FNLKGKLKPN VSGKDVILHI
IGMIGVDGAL YKSMEFRGEG VSSLTMDDRA CIANMAIEAG AKNGIFEVDD QTIEYLKDIV
KRDYTVFKAD DDAVYDKEYD IDLSLIEPTV ACPHLPENTK EAKELKNIKV DQVVIGSCTN
GRLSDMATAA NILKGKKVAK GVRCIVIPAT QKVYKECIKL GYMDIFIDAG CAVSTPTCGP
CLGGYMGILA HDEVAVTTTN RNFVGRMGDK TSKVYLASPA TAAYSAITGY ITEPK
//
