ID C3ZAH8_BRAFL Unreviewed; 2208 AA.
AC C3ZAH8;
DT 28-JUL-2009, integrated into UniProtKB/TrEMBL.
DT 28-JUL-2009, sequence version 1.
DT 27-MAR-2024, entry version 92.
DE RecName: Full=non-specific serine/threonine protein kinase {ECO:0000256|ARBA:ARBA00012513};
DE EC=2.7.11.1 {ECO:0000256|ARBA:ARBA00012513};
GN ORFNames=BRAFLDRAFT_119973 {ECO:0000313|EMBL:EEN50381.1};
OS Branchiostoma floridae (Florida lancelet) (Amphioxus).
OC Eukaryota; Metazoa; Chordata; Cephalochordata; Leptocardii; Amphioxiformes;
OC Branchiostomatidae; Branchiostoma.
OX NCBI_TaxID=7739;
RN [1] {ECO:0000313|EMBL:EEN50381.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S238N-H82 {ECO:0000313|EMBL:EEN50381.1};
RC TISSUE=Testes {ECO:0000313|EMBL:EEN50381.1};
RX PubMed=18563158; DOI=10.1038/nature06967;
RG US DOE Joint Genome Institute (JGI-PGF);
RA Putnam N.H., Butts T., Ferrier D.E.K., Furlong R.F., Hellsten U.,
RA Kawashima T., Robinson-Rechavi M., Shoguchi E., Terry A., Yu J.-K.,
RA Benito-Gutierrez E.L., Dubchak I., Garcia-Fernandez J., Gibson-Brown J.J.,
RA Grigoriev I.V., Horton A.C., de Jong P.J., Jurka J., Kapitonov V.V.,
RA Kohara Y., Kuroki Y., Lindquist E., Lucas S., Osoegawa K., Pennacchio L.A.,
RA Salamov A.A., Satou Y., Sauka-Spengler T., Schmutz J., Shin-I T.,
RA Toyoda A., Bronner-Fraser M., Fujiyama A., Holland L.Z., Holland P.W.H.,
RA Satoh N., Rokhsar D.S.;
RT "The amphioxus genome and the evolution of the chordate karyotype.";
RL Nature 453:1064-1071(2008).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001433};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1; Evidence={ECO:0000256|ARBA:ARBA00000775};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
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DR EMBL; GG666602; EEN50381.1; -; Genomic_DNA.
DR RefSeq; XP_002594370.1; XM_002594324.1.
DR eggNOG; KOG0192; Eukaryota.
DR eggNOG; KOG0619; Eukaryota.
DR InParanoid; C3ZAH8; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR GO; GO:0009966; P:regulation of signal transduction; IEA:UniProt.
DR Gene3D; 1.25.40.20; Ankyrin repeat-containing domain; 1.
DR Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 2.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 3.80.10.10; Ribonuclease Inhibitor; 1.
DR Gene3D; 3.30.70.1390; ROC domain from the Parkinson's disease-associated leucine-rich repeat kinase 2; 1.
DR Gene3D; 1.10.510.10; Transferase(Phosphotransferase) domain 1; 1.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR032171; COR.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR020859; ROC_dom.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR48056:SF81; LEUCINE-RICH REPEAT CONTAINING PROTEIN; 1.
DR PANTHER; PTHR48056; LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED; 1.
DR Pfam; PF16095; COR; 1.
DR Pfam; PF00560; LRR_1; 1.
DR Pfam; PF13855; LRR_8; 2.
DR Pfam; PF00069; Pkinase; 1.
DR Pfam; PF08477; Roc; 1.
DR PRINTS; PR00449; RASTRNSFRMNG.
DR SMART; SM00248; ANK; 2.
DR SMART; SM00185; ARM; 4.
DR SMART; SM00364; LRR_BAC; 5.
DR SMART; SM00369; LRR_TYP; 7.
DR SMART; SM00175; RAB; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF48403; Ankyrin repeat; 1.
DR SUPFAM; SSF48371; ARM repeat; 2.
DR SUPFAM; SSF52058; L domain-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR PROSITE; PS51450; LRR; 2.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
DR PROSITE; PS51424; ROC; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU10141}; Differentiation {ECO:0000256|ARBA:ARBA00022782};
KW GTP-binding {ECO:0000256|ARBA:ARBA00023134};
KW Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Leucine-rich repeat {ECO:0000256|ARBA:ARBA00022614};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU10141}; Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Serine/threonine-protein kinase {ECO:0000256|ARBA:ARBA00022527};
KW Transferase {ECO:0000256|ARBA:ARBA00022777}.
FT DOMAIN 1378..1565
FT /note="Roc"
FT /evidence="ECO:0000259|PROSITE:PS51424"
FT DOMAIN 1924..2182
FT /note="Protein kinase"
FT /evidence="ECO:0000259|PROSITE:PS50011"
FT REGION 878..918
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 952..1048
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 952..983
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1009..1027
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 1951
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10141"
SQ SEQUENCE 2208 AA; 248069 MW; C71181C1E7E5F714 CRC64;
MEPLAPAETK KDRTLDLMLV LKEHFDPKQI CSVLNELMGL VEDGPSLECM EDYHVHQLIL
DLMTQFKTNP DVQEVGCNAI WKLFHHSSRQ RKRIDKAEAY KLVMEAMERH KQVEAVQAAG
LRCAAYLAIS RTTRASMLEN EIMDQVIIAM TAFKENPSVQ ANACQALTHL LFDDERHLTE
LVSSKGYKLI VEAAAQHADC AAVMEQVLWA LRVLVTPEVN RRMLETERLY RYATSAMGRF
AHEGEVQAAA CGVIEMLSTD GDALNASDDE DTFESRLVTG QNISWLEDIF SALTNHASDP
AVQEAGCRSL CKLLEHHPDL IKHIGEEDNQ MPIHSAVLAA IMMHGEDQYV FKVACEAIYH
LTAEWVLFCC CVIAEPLQTL MMNKGAYLSI LNYMGHHATI PGAQQFACRA LRGLTLLNDD
HKQKLAEVSA LSLVASALKR FRDDVGVQEE AIGVIATMSD VVRHQCIIEK VHTIVLDSMN
KFLLSRDVQD LGLEALGVLS LAAGVPAMLN EAGALHTTIH AMEKNPEDDS IQYKGLIMLQ
VLVDEERFNS PQVCEEVGKL VVEAMSRFKD IIRIQEEGCV AIQLLAEMST DMSQVLVNND
AQECLFHILE CYSYNQGLLD VASECLYVLS CELDLKPQML LSACSKGSIR GVECLIQLGA
DVNTGEGHNT PLCLACERGD EHLVHFLLKQ LSLKKEHHSI IGLLLKHMGH DREAGVVSWS
GLSLGGLRAE WLQPMLVDRN ITKNMDGSGV AMAAMVKHVR NRREMRPLPL TRNHFGGSDP
SLAKMSFPLD DSTDRRFPLY RSVARPDDTN QASITSDTSF DGYEVQDIRR KSFAYARSPS
EDSLLDDRID ASRSDKWWNR MLYPIFSVFS ELTGLGDSSR RLSGESDLEL TPAKPRKKLR
RKYSKTGSEM PFNRWDPRNM TEKKFGQALA TIPQGKDRPL VTASSLGFSP FAQSRTGSSV
DSSPTQLTPV GTPYTSTPIR SPSPNVLRRE RTWVSISEAE ETDSTVEEPL SPQRDSMDQS
KSPSLAVPSQ PHADLPHKDS MDQVSPGQVK KLSLMKIEED NKQSFRKRAF TSADKPVRSL
STNTVRLLDI SANGIVSLDG LTKSDTNILN SLVAVEKLDL SQNSLKDFPW RLCQALPKLQ
SLDLHSNSFT TFPAHVLACQ HLDSLDLSHN KIADLTKKPS KTSFSLKEFN ISHNSLTALP
SWIGICMPML QVLNVEGNRI ANIDDEDSFQ LRRLKFVNIS KNQLKELPLK FFLGCPKLET
LVASCNELDN LPYDAAMKLQ NLSTVKLAHN KLAEPEPTYI PRFLLLLDRL RSLDLSNNEL
QGLPPPSHWN THALRELMLG GNHIRKLPLD GLRLDLDPAI VKGRTKDVIG FLSQRLKQAV
PHYRMKLMLV GFGGRGKSTL LRQLQKIKGD PRFNTATIGV LVKDWKIPVK KGRSKTHTYT
LSTWDFAGQE EFYSTHQCFL SSRSLYLVVY DLSRGPKEVE SLRPWLLNIQ ARAPNCPVIV
VGTHKDRIKR EEADTIILEM RHRVQTLCTT PGFPEIKGYS EVSCIKESPA MEDLRKQIIT
VIDNFKVKGQ PVMGQMIPHS YMLLEDLVTE EAQSLKAGLP VIRHSRLLEL VKEANLQLDE
EELSQAVRFL HESGVLLHYD DPALQLKDLY FIDPEWLCQM MAQVVTVREG NPFVNNKGIL
KKSDVRNLFK GGAFPPELIP QYLRLLEKFE IALPQTEEEL LIPCRLPFVQ PPIDKTAWFT
GHVYRQYVMP YIPIGYWSRL ITRLMVFSMY LKTGPLPPLQ QEYWREGIYV FWSTQEYFLV
KRIKGEKNSM DITVPATRQG SRLLGHVVDL VDDLIEEWFP GLCEIDPVSG QTLVQRQVPC
VLCETDPPHV FTLPEILVMS DRDDIIFCPN HDMPVPLHKL APDVMLSDLD SRYQLQEDKF
KLEQIPENEL GDGSFGNVYR ASYKNMDVAV KVFSKVGEDH PHRMLRQEVT ILTRLQHISV
ISLLAVGIRP RLLVLELAPM GSLTSHLCKK MSRGLQQRIA MQIAEGLMFL HTNMIIYRDL
KPDNILIFSI AQNSPVNAKI SDYGISKFAT LLGLTAPEGT PGYRAPEVAR GDTAYNNKVD
LFSFGILLFE LVTGRKPFED LHFRGELDEA IIKGRPLDPI TSCGSAPWPD VQDLITHCMQ
PIPDDRPTAA EVFARLSSGE LLCLRRNVPL GKGLSPECIA IRVGGTMK
//
