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Database: UniProt/TrEMBL
Entry: C4R142_KOMPG
LinkDB: C4R142_KOMPG
Original site: C4R142_KOMPG 
ID   C4R142_KOMPG            Unreviewed;       432 AA.
AC   C4R142;
DT   07-JUL-2009, integrated into UniProtKB/TrEMBL.
DT   07-JUL-2009, sequence version 1.
DT   07-JUN-2017, entry version 56.
DE   RecName: Full=Isocitrate dehydrogenase [NADP] {ECO:0000256|PIRNR:PIRNR000108};
DE            EC=1.1.1.42 {ECO:0000256|PIRNR:PIRNR000108};
GN   OrderedLocusNames=PAS_chr2-1_0580 {ECO:0000313|EMBL:CAY69216.1};
OS   Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) (Pichia
OS   pastoris).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Phaffomycetaceae; Komagataella.
OX   NCBI_TaxID=644223 {ECO:0000313|EMBL:CAY69216.1, ECO:0000313|Proteomes:UP000000314};
RN   [1] {ECO:0000313|EMBL:CAY69216.1, ECO:0000313|Proteomes:UP000000314}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GS115 / ATCC 20864 {ECO:0000313|Proteomes:UP000000314};
RX   PubMed=19465926; DOI=10.1038/nbt.1544;
RA   De Schutter K., Lin Y.-C., Tiels P., Van Hecke A., Glinka S.,
RA   Weber-Lehmann J., Rouze P., Van de Peer Y., Callewaert N.;
RT   "Genome sequence of the recombinant protein production host Pichia
RT   pastoris.";
RL   Nat. Biotechnol. 27:561-566(2009).
CC   -!- CATALYTIC ACTIVITY: Isocitrate + NADP(+) = 2-oxoglutarate + CO(2)
CC       + NADPH. {ECO:0000256|PIRNR:PIRNR000108}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|PIRNR:PIRNR000108,
CC         ECO:0000256|PIRSR:PIRSR000108-3};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000256|PIRNR:PIRNR000108,
CC         ECO:0000256|PIRSR:PIRSR000108-3};
CC       Note=Binds 1 Mg(2+) or Mn(2+) ion per subunit.
CC       {ECO:0000256|PIRNR:PIRNR000108, ECO:0000256|PIRSR:PIRSR000108-3};
CC   -!- SIMILARITY: Belongs to the isocitrate and isopropylmalate
CC       dehydrogenases family. {ECO:0000256|PIRNR:PIRNR000108}.
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DR   EMBL; FN392320; CAY69216.1; -; Genomic_DNA.
DR   RefSeq; XP_002491496.1; XM_002491451.1.
DR   ProteinModelPortal; C4R142; -.
DR   STRING; 644223.XP_002491496.1; -.
DR   EnsemblFungi; CAY69216; CAY69216; PAS_chr2-1_0580.
DR   GeneID; 8198933; -.
DR   KEGG; ppa:PAS_chr2-1_0580; -.
DR   eggNOG; KOG1526; Eukaryota.
DR   eggNOG; COG0538; LUCA.
DR   HOGENOM; HOG000019858; -.
DR   InParanoid; C4R142; -.
DR   KO; K00031; -.
DR   OMA; YLNTMDF; -.
DR   OrthoDB; EOG092C2D51; -.
DR   Proteomes; UP000000314; Chromosome 2.
DR   GO; GO:0004450; F:isocitrate dehydrogenase (NADP+) activity; IEA:UniProtKB-EC.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0006102; P:isocitrate metabolic process; IEA:InterPro.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   InterPro; IPR019818; IsoCit/isopropylmalate_DH_CS.
DR   InterPro; IPR004790; Isocitrate_DH_NADP.
DR   InterPro; IPR024084; IsoPropMal-DH-like_dom.
DR   PANTHER; PTHR11822; PTHR11822; 1.
DR   Pfam; PF00180; Iso_dh; 1.
DR   PIRSF; PIRSF000108; IDH_NADP; 1.
DR   SMART; SM01329; Iso_dh; 1.
DR   TIGRFAMs; TIGR00127; nadp_idh_euk; 1.
DR   PROSITE; PS00470; IDH_IMDH; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000000314};
KW   Magnesium {ECO:0000256|PIRNR:PIRNR000108,
KW   ECO:0000256|PIRSR:PIRSR000108-3};
KW   Manganese {ECO:0000256|PIRNR:PIRNR000108,
KW   ECO:0000256|PIRSR:PIRSR000108-3};
KW   Metal-binding {ECO:0000256|PIRNR:PIRNR000108,
KW   ECO:0000256|PIRSR:PIRSR000108-3};
KW   NADP {ECO:0000256|PIRNR:PIRNR000108, ECO:0000256|PIRSR:PIRSR000108-4};
KW   Oxidoreductase {ECO:0000256|PIRNR:PIRNR000108};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000314};
KW   Signal {ECO:0000256|SAM:SignalP};
KW   Tricarboxylic acid cycle {ECO:0000256|PIRNR:PIRNR000108}.
FT   SIGNAL        1     24       {ECO:0000256|SAM:SignalP}.
FT   CHAIN        25    432       Isocitrate dehydrogenase [NADP].
FT                                {ECO:0000256|SAM:SignalP}.
FT                                /FTId=PRO_5002942154.
FT   DOMAIN       31    420       Iso_dh. {ECO:0000259|SMART:SM01329}.
FT   NP_BIND      97     99       NADP. {ECO:0000256|PIRSR:PIRSR000108-4}.
FT   NP_BIND     331    336       NADP. {ECO:0000256|PIRSR:PIRSR000108-4}.
FT   REGION      116    122       Substrate binding. {ECO:0000256|PIRSR:
FT                                PIRSR000108-2}.
FT   METAL       273    273       Magnesium or manganese.
FT                                {ECO:0000256|PIRSR:PIRSR000108-3}.
FT   METAL       296    296       Magnesium or manganese.
FT                                {ECO:0000256|PIRSR:PIRSR000108-3}.
FT   BINDING      99     99       Substrate. {ECO:0000256|PIRSR:
FT                                PIRSR000108-2}.
FT   BINDING     104    104       NADP. {ECO:0000256|PIRSR:PIRSR000108-4}.
FT   BINDING     131    131       Substrate. {ECO:0000256|PIRSR:
FT                                PIRSR000108-2}.
FT   BINDING     154    154       Substrate. {ECO:0000256|PIRSR:
FT                                PIRSR000108-2}.
FT   BINDING     281    281       NADP. {ECO:0000256|PIRSR:PIRSR000108-4}.
FT   BINDING     349    349       NADP; via amide nitrogen and carbonyl
FT                                oxygen. {ECO:0000256|PIRSR:PIRSR000108-
FT                                4}.
FT   SITE        161    161       Critical for catalysis.
FT                                {ECO:0000256|PIRSR:PIRSR000108-1}.
FT   SITE        233    233       Critical for catalysis.
FT                                {ECO:0000256|PIRSR:PIRSR000108-1}.
SQ   SEQUENCE   432 AA;  48755 MW;  17B43CA9069AFC76 CRC64;
     MGKVTVFHLF FFFLGISYCK MAHAKISVKT PLVEMDGDEM TRIIWKLIKD ELILPFLDID
     LKYYDLGIEY RDQTDDQVTI DAAEAIKKYG VGVKCATITP DEARVEEFGL KKMWLSPNGT
     IRNILGGTVF REPIVIDNIP RIIPQWEKPI IIGRHAYGDQ YRATDLLIPK AGELKLVFTP
     KDGSDPVETK VFDYPSAGVA LTMYNLDDSI RDFALSSFKL ALEKKVNLFS TTKNTILKKY
     DGRFKDIFDE TYETQFKESF EKAGIWYEHR LIDDMVAQML KSKGGYIIAM KNYDGDVQSD
     IVAQGFGSLG LMTSVLTTPD GTAFESEAAH GTVTRHYRQH QQGKETSTNS IASIFAWTRG
     LIQRGLLDNT LPVVEFGQLL ENATINTVKL DGIMTKDLAL ARGETDRSSY VNTEEFIKAV
     AKRLTSEFEA KF
//
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