ID C4X0Q5_KLEPN Unreviewed; 431 AA.
AC C4X0Q5;
DT 28-JUL-2009, integrated into UniProtKB/TrEMBL.
DT 28-JUL-2009, sequence version 1.
DT 03-APR-2013, entry version 31.
DE RecName: Full=Ribosomal RNA small subunit methyltransferase B;
DE EC=2.1.1.176;
DE AltName: Full=16S rRNA m5C967 methyltransferase;
DE AltName: Full=rRNA (cytosine-C(5)-)-methyltransferase RsmB;
GN Name=rrmB; Synonyms=rsmB, sun; ORFNames=KP1_5007;
OS Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Klebsiella.
OX NCBI_TaxID=484021;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=NTUH-K2044;
RA Wu K.M., Tsai S.F.;
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=NTUH-K2044;
RX PubMed=19447910; DOI=10.1128/JB.00315-09;
RA Wu K.-M., Li L.-H., Yan J.-J., Tsao N., Liao T.-L., Tsai H.-C.,
RA Fung C.-P., Chen H.-J., Liu Y.-M., Wang J.-T., Fang C.-T.,
RA Chang S.-C., Shu H.-Y., Liu T.-T., Chen Y.-T., Shiau Y.-R.,
RA Lauderdale T.-L., Su I.-J., Kirby R., Tsai S.-F.;
RT "Genome sequencing and comparative analysis of Klebsiella pneumoniae
RT NTUH-K2044, a strain causing liver abscess and meningitis.";
RL J. Bacteriol. 191:4492-4501(2009).
CC -!- FUNCTION: Specifically methylates the cytosine at position 967
CC (m5C967) of 16S rRNA (By similarity).
CC -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + cytosine(967) in 16S
CC rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(967) in 16S
CC rRNA.
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. RsmB/NOP
CC family.
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DR EMBL; AP006725; BAH65463.1; -; Genomic_DNA.
DR RefSeq; YP_002921530.1; NC_012731.1.
DR ProteinModelPortal; C4X0Q5; -.
DR STRING; 484021.KP1_5007; -.
DR EnsemblBacteria; BAH65463; BAH65463; KP1_5007.
DR GeneID; 7947380; -.
DR KEGG; kpu:KP1_5007; -.
DR PATRIC; 20453247; VBIKlePne21779_4392.
DR eggNOG; COG0144; -.
DR KO; K03500; -.
DR ProtClustDB; PRK10901; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0016434; F:rRNA (cytosine) methyltransferase activity; IEA:HAMAP.
DR GO; GO:0006355; P:regulation of transcription, DNA-dependent; IEA:InterPro.
DR Gene3D; 1.10.940.10; -; 1.
DR HAMAP; MF_01856; 16SrRNA_methyltr_B; 1; -.
DR InterPro; IPR001678; Fmu/NOL1/Nop2p.
DR InterPro; IPR018314; Fmu/NOL1/Nop2p_CS.
DR InterPro; IPR006027; NusB_RsmB_TIM44.
DR InterPro; IPR023267; RCMT.
DR InterPro; IPR004573; rRNA_ssu_MeTfrase_B.
DR InterPro; IPR023541; rRNA_ssu_MeTfrase_B_ent.
DR Pfam; PF01189; Nol1_Nop2_Fmu; 1.
DR Pfam; PF01029; NusB; 1.
DR PRINTS; PR02008; RCMTFAMILY.
DR SUPFAM; SSF48013; NusB_RsmB_TIM44; 1.
DR TIGRFAMs; TIGR00563; rsmB; 1.
DR PROSITE; PS01153; NOL1_NOP2_SUN; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Methyltransferase; RNA-binding; rRNA processing;
KW S-adenosyl-L-methionine; Transferase.
FT REGION 254 260 S-adenosyl-L-methionine binding (By
FT similarity).
FT ACT_SITE 375 375 Nucleophile (By similarity).
FT BINDING 277 277 S-adenosyl-L-methionine (By similarity).
FT BINDING 303 303 S-adenosyl-L-methionine (By similarity).
FT BINDING 322 322 S-adenosyl-L-methionine (By similarity).
SQ SEQUENCE 431 AA; 48617 MW; 2FD4A202B124BD33 CRC64;
MKKNINLRSL AAQAIEQVVE KGQSLSNVLP PLQQKVSDKD KALLQELCFG VLRTLSQLEW
LISKLMARPM TGKQRTVHFL IMVGLYQLLY TRIPPHAALA ETVEGAVAIK RPQLKGLING
VLRQFQRQQE ALLAEFAEHE NRYLHPKWLL KRLQQAWPQQ WQEIVEANNQ RPPMWLRVNR
NHHSRDEWLA LLNETGLEGF THPDYPDAVR LATPAPVHAL PGFAEGWVTV QDASAQGCMR
YLQPENGERI LDLCAAPGGK TTHILEVAPQ AQVMAVDIDE QRLSRVYDNL KRLGVKAEVK
QGDGRFPEQW CGNEQFDRIL LDAPCSATGV IRRHPDIKWL RRDRDIAELA QLQAEILNAT
WTHLKPGGTL VYATCSILPE ENSQQIAAFL ARTPDAELHA TGTPASPGQQ NLPGVEEGDG
FFYAKLIKRR N
//
