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Database: UniProt/TrEMBL
Entry: C5A7K8_THEGJ
LinkDB: C5A7K8_THEGJ
Original site: C5A7K8_THEGJ 
ID   C5A7K8_THEGJ            Unreviewed;       559 AA.
AC   C5A7K8;
DT   28-JUL-2009, integrated into UniProtKB/TrEMBL.
DT   28-JUL-2009, sequence version 1.
DT   25-OCT-2017, entry version 63.
DE   RecName: Full=DNA ligase {ECO:0000256|HAMAP-Rule:MF_00407};
DE            EC=6.5.1.6 {ECO:0000256|HAMAP-Rule:MF_00407};
DE   AltName: Full=Polydeoxyribonucleotide synthase [ATP/NAD(+)] {ECO:0000256|HAMAP-Rule:MF_00407};
GN   Name=lig {ECO:0000256|HAMAP-Rule:MF_00407,
GN   ECO:0000313|EMBL:ACS34220.1};
GN   OrderedLocusNames=TGAM_1718 {ECO:0000313|EMBL:ACS34220.1};
OS   Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Thermococcus.
OX   NCBI_TaxID=593117 {ECO:0000313|EMBL:ACS34220.1, ECO:0000313|Proteomes:UP000001488};
RN   [1] {ECO:0000313|EMBL:ACS34220.1, ECO:0000313|Proteomes:UP000001488}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 15229 / JCM 11827 / EJ3
RC   {ECO:0000313|Proteomes:UP000001488};
RX   PubMed=19558674; DOI=10.1186/gb-2009-10-6-r70;
RA   Zivanovic Y., Armengaud J., Lagorce A., Leplat C., Guerin P.,
RA   Dutertre M., Anthouard V., Forterre P., Wincker P., Confalonieri F.;
RT   "Genome analysis and genome-wide proteomics of Thermococcus
RT   gammatolerans, the most radioresistant organism known amongst the
RT   Archaea.";
RL   Genome Biol. 10:R70.1-R70.23(2007).
CC   -!- FUNCTION: DNA ligase that seals nicks in double-stranded DNA
CC       during DNA replication, DNA recombination and DNA repair.
CC       {ECO:0000256|HAMAP-Rule:MF_00407}.
CC   -!- CATALYTIC ACTIVITY: ATP + (deoxyribonucleotide)(n)-3'-hydroxyl +
CC       5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) +
CC       AMP + diphosphate. {ECO:0000256|HAMAP-Rule:MF_00407}.
CC   -!- CATALYTIC ACTIVITY: NAD(+) + (deoxyribonucleotide)(n)-3'-hydroxyl
CC       + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m)
CC       + AMP + beta-nicotinamide D-nucleotide. {ECO:0000256|HAMAP-
CC       Rule:MF_00407}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_00407};
CC   -!- SIMILARITY: Belongs to the ATP-dependent DNA ligase family.
CC       {ECO:0000256|HAMAP-Rule:MF_00407, ECO:0000256|RuleBase:RU004196}.
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DR   EMBL; CP001398; ACS34220.1; -; Genomic_DNA.
DR   RefSeq; WP_015859330.1; NC_012804.1.
DR   STRING; 593117.TGAM_1718; -.
DR   PaxDb; C5A7K8; -.
DR   PRIDE; C5A7K8; -.
DR   EnsemblBacteria; ACS34220; ACS34220; TGAM_1718.
DR   GeneID; 7987437; -.
DR   KEGG; tga:TGAM_1718; -.
DR   PATRIC; fig|593117.10.peg.1725; -.
DR   eggNOG; arCOG01347; Archaea.
DR   eggNOG; COG1793; LUCA.
DR   HOGENOM; HOG000036008; -.
DR   KO; K10747; -.
DR   OMA; ETVCNIG; -.
DR   OrthoDB; POG093Z03L0; -.
DR   Proteomes; UP000001488; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003910; F:DNA ligase (ATP) activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0071897; P:DNA biosynthetic process; IEA:InterPro.
DR   GO; GO:0051103; P:DNA ligation involved in DNA repair; IEA:InterPro.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.3260.10; -; 1.
DR   HAMAP; MF_00407; DNA_ligase; 1.
DR   InterPro; IPR022865; DNA_ligae_ATP-dep_bac/arc.
DR   InterPro; IPR000977; DNA_ligase_ATP-dep.
DR   InterPro; IPR012309; DNA_ligase_ATP-dep_C.
DR   InterPro; IPR012310; DNA_ligase_ATP-dep_cent.
DR   InterPro; IPR016059; DNA_ligase_ATP-dep_CS.
DR   InterPro; IPR012308; DNA_ligase_ATP-dep_N.
DR   InterPro; IPR036599; DNA_ligase_N_sf.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   Pfam; PF04679; DNA_ligase_A_C; 1.
DR   Pfam; PF01068; DNA_ligase_A_M; 1.
DR   Pfam; PF04675; DNA_ligase_A_N; 1.
DR   SUPFAM; SSF117018; SSF117018; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   TIGRFAMs; TIGR00574; dnl1; 1.
DR   PROSITE; PS00697; DNA_LIGASE_A1; 1.
DR   PROSITE; PS00333; DNA_LIGASE_A2; 1.
DR   PROSITE; PS50160; DNA_LIGASE_A3; 1.
PE   3: Inferred from homology;
KW   ATP-binding {ECO:0000256|HAMAP-Rule:MF_00407};
KW   Cell cycle {ECO:0000256|HAMAP-Rule:MF_00407};
KW   Cell division {ECO:0000256|HAMAP-Rule:MF_00407};
KW   Complete proteome {ECO:0000313|Proteomes:UP000001488};
KW   DNA damage {ECO:0000256|HAMAP-Rule:MF_00407};
KW   DNA recombination {ECO:0000256|HAMAP-Rule:MF_00407};
KW   DNA repair {ECO:0000256|HAMAP-Rule:MF_00407};
KW   DNA replication {ECO:0000256|HAMAP-Rule:MF_00407};
KW   Ligase {ECO:0000256|HAMAP-Rule:MF_00407, ECO:0000313|EMBL:ACS34220.1};
KW   Magnesium {ECO:0000256|HAMAP-Rule:MF_00407};
KW   Metal-binding {ECO:0000256|HAMAP-Rule:MF_00407};
KW   NAD {ECO:0000256|HAMAP-Rule:MF_00407};
KW   Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_00407}.
FT   DOMAIN      327    455       DNA_LIGASE_A3. {ECO:0000259|PROSITE:
FT                                PS50160}.
FT   ACT_SITE    249    249       N6-AMP-lysine intermediate.
FT                                {ECO:0000256|HAMAP-Rule:MF_00407}.
FT   BINDING     247    247       ATP. {ECO:0000256|HAMAP-Rule:MF_00407}.
FT   BINDING     254    254       ATP. {ECO:0000256|HAMAP-Rule:MF_00407}.
FT   BINDING     269    269       ATP. {ECO:0000256|HAMAP-Rule:MF_00407}.
FT   BINDING     299    299       ATP. {ECO:0000256|HAMAP-Rule:MF_00407}.
FT   BINDING     339    339       ATP. {ECO:0000256|HAMAP-Rule:MF_00407}.
FT   BINDING     414    414       ATP. {ECO:0000256|HAMAP-Rule:MF_00407}.
FT   BINDING     420    420       ATP. {ECO:0000256|HAMAP-Rule:MF_00407}.
SQ   SEQUENCE   559 AA;  63507 MW;  FD51939B52FD9EDC CRC64;
     MRYSELAELY RRLEKTTLKT LKTKFVADFL KKTPDDLLEI VPYLILGKVF PDWDERELGV
     GEKLLIKAVS MATGVPEKEI ENSIKDTGDL GESVALALKK RRQKSFFSQP LTIKRVYNTF
     VKVAEASGEG SQDRKMKYLA NLFMDAGPEE GKYIARTVLG TMRTGVAEGI LRDAIAEAFK
     VKVELVERAY MLTSDFGYVA KIAKLEGNEG LSKVSIQIGK PIRPMLAQNA ASVKDALIEM
     GGEAAFEIKY DGARVQVHRD GDKVIIYSRR LENVTRSIPE IVEAVKASLK PSKVIVEGEL
     VAVGENGRPR PFQYVLRRFR RKYNIEEMIE KIPLELNLFD ILYVNGESLI DTKFTERRKR
     LEESVEESDK IKLAEQLVTK KVDEAEEFYK RALELGHEGL MAKRLDAIYE PGNRGKKWLK
     IKPTMENLDL VIIGAEWGEG RRAHLLGSFL VGAYDPESGE FVPVGKVGSG FTDEDLVEFT
     KMLKPLIIRE EGKFVEIEPK VVIEVTYQEI QKSPKYKSGF ALRFPRYVAL REDKSPEEAD
     TIERVAQLYE LQERFKAKR
//
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