ID C6AKC8_AGGAN Unreviewed; 757 AA.
AC C6AKC8;
DT 01-SEP-2009, integrated into UniProtKB/TrEMBL.
DT 01-SEP-2009, sequence version 1.
DT 01-MAY-2013, entry version 30.
DE RecName: Full=Glutathione biosynthesis bifunctional protein GshAB;
DE AltName: Full=Gamma-GCS-GS;
GN Name=gshAB; Synonyms=gshF; OrderedLocusNames=NT05HA_0176;
OS Aggregatibacter aphrophilus (strain NJ8700) (Haemophilus aphrophilus).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Aggregatibacter.
OX NCBI_TaxID=634176;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NJ8700;
RX PubMed=19447908; DOI=10.1128/JB.00447-09;
RA Di Bonaventura M.P., DeSalle R., Pop M., Nagarajan N., Figurski D.H.,
RA Fine D.H., Kaplan J.B., Planet P.J.;
RT "Complete genome sequence of Aggregatibacter (Haemophilus) aphrophilus
RT NJ8700.";
RL J. Bacteriol. 191:4693-4694(2009).
CC -!- FUNCTION: Synthesizes glutathione from L-glutamate and L-cysteine
CC via gamma-L-glutamyl-L-cysteine (By similarity).
CC -!- CATALYTIC ACTIVITY: ATP + L-glutamate + L-cysteine = ADP +
CC phosphate + gamma-L-glutamyl-L-cysteine.
CC -!- CATALYTIC ACTIVITY: ATP + gamma-L-glutamyl-L-cysteine + glycine =
CC ADP + phosphate + glutathione.
CC -!- PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione
CC from L-cysteine and L-glutamate: step 1/2.
CC -!- PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione
CC from L-cysteine and L-glutamate: step 2/2.
CC -!- SUBUNIT: Monomer (By similarity).
CC -!- SIMILARITY: Contains 1 ATP-grasp domain.
CC -!- SIMILARITY: In the N-terminal section; belongs to the glutamate--
CC cysteine ligase type 1 family. Type 2 subfamily.
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DR EMBL; CP001607; ACS96614.1; -; Genomic_DNA.
DR RefSeq; YP_003006701.1; NC_012913.1.
DR STRING; 634176.NT05HA_0176; -.
DR EnsemblBacteria; ACS96614; ACS96614; NT05HA_0176.
DR GeneID; 8121538; -.
DR KEGG; aap:NT05HA_0176; -.
DR PATRIC; 31915474; VBIAggAph86045_0170.
DR eggNOG; COG1181; -.
DR HOGENOM; HOG000156471; -.
DR KO; K01919; -.
DR OMA; PYIQTDF; -.
DR ProtClustDB; PRK02471; -.
DR BioCyc; AAPH634176:GHVL-173-MONOMER; -.
DR UniPathway; UPA00142; UER00209.
DR GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR GO; GO:0004357; F:glutamate-cysteine ligase activity; IEA:HAMAP.
DR GO; GO:0004363; F:glutathione synthase activity; IEA:HAMAP.
DR GO; GO:0000287; F:magnesium ion binding; IEA:HAMAP.
DR GO; GO:0030145; F:manganese ion binding; IEA:HAMAP.
DR Gene3D; 3.30.470.20; -; 2.
DR HAMAP; MF_00782; Glut_biosynth; 1; -.
DR InterPro; IPR011761; ATP-grasp.
DR InterPro; IPR013816; ATP_grasp_subdomain_2.
DR InterPro; IPR007370; Glu_cys_ligase.
DR InterPro; IPR006335; Glut_cys-rel.
DR Pfam; PF04262; Glu_cys_ligase; 1.
DR TIGRFAMs; TIGR01435; glu_cys_lig_rel; 1.
DR PROSITE; PS50975; ATP_GRASP; 1.
PE 3: Inferred from homology;
KW ATP-binding; Complete proteome; Glutathione biosynthesis; Ligase;
KW Magnesium; Manganese; Metal-binding; Multifunctional enzyme;
KW Nucleotide-binding.
FT DOMAIN 494 753 ATP-grasp (By similarity).
FT NP_BIND 521 580 ATP (By similarity).
FT REGION 1 337 Glutamate--cysteine ligase (By
FT similarity).
FT METAL 702 702 Magnesium or manganese 1 (By similarity).
FT METAL 723 723 Magnesium or manganese 1 (By similarity).
FT METAL 723 723 Magnesium or manganese 2 (By similarity).
FT METAL 725 725 Magnesium or manganese 2 (By similarity).
SQ SEQUENCE 757 AA; 85093 MW; 0EA68ECF97BA7512 CRC64;
MNIQQVIKEN RLELLFQQGS FGIEKESQRV HADGSIVVTE HPKCFGSRSY HPYIQTDFAE
SQLELITPPN KKIEDSLRWL SAIHEVVLCS LPEDEFIFPL SMPAGLPPED QIKVAQLENP
ADVAYREHLV ASYGKNKQMV SGIHYNFQLE PTFIQQLFAQ QNTYQSAVDF QNDLYLKVAR
NFLRYQWILV YLLAATPTVD ANYFRHGTPL KAGQYVRSLR SSQYGYVNDP SVKVSYDSLN
DYVKTLEHAV SSGQLIAEKE FYSNVRLRGA KHARELLQNG IQYLEFRLFD LNPFEAYGIA
LNDAKFVHYF ILLMAWLDEE SLASAVDLGK EKLAQVAWEN PLSATAFQAE GERILQQLLA
MLTEINADVE IEAIVKDKLV QFAEPSQTLG ARLVKAIEAH GGYQKLGAEL AIRYKQRAFE
RFYALSAFDN MELSTQALMF DAIQKGLKME ILDERDQFLS LQFGDHLEYV KNGNMTSHDS
YISPLIMENK VVTKKVLAKA GFNVPQSVEF TSVEQAVANY ALFAGRAVVI KPKSTNYGLG
ISIFQQGVHD REDFAKAIEI AFREDKEVMV EDYLTGTEYR FFVLGDETLA VLLRVPANVI
GDSVHTVAEL VAQKNDHPLR GDGSRTPLKK IALGDIEQLQ LKEQGLTVDS VPAKDQLVQL
RANSNISTGG DSIDMTDQMH PSYKALAVGI TKAMGAAVCG VDLIIPDLTK PAEPSLQSWG
VIEANFNPMM MMHIFPYAGQ SRRVTQNVLK MLFPELK
//
