ID   C6V1W7_ECO5T            Unreviewed;       533 AA.
AC   C6V1W7;
DT   22-SEP-2009, integrated into UniProtKB/TrEMBL.
DT   22-SEP-2009, sequence version 1.
DT   01-MAY-2013, entry version 31.
DE   RecName: Full=Malate synthase;
DE            EC=2.3.3.9;
GN   Name=aceB; OrderedLocusNames=ECSP_5083;
OS   Escherichia coli O157:H7 (strain TW14359 / EHEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=544404;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TW14359 / EHEC;
RX   PubMed=19564389; DOI=10.1128/IAI.00198-09;
RA   Kulasekara B.R., Jacobs M., Zhou Y., Wu Z., Sims E.,
RA   Saenphimmachak C., Rohmer L., Ritchie J.M., Radey M., McKevitt M.,
RA   Freeman T.L., Hayden H., Haugen E., Gillett W., Fong C., Chang J.,
RA   Beskhlebnaya V., Waldor M.K., Samadpour M., Whittam T.S., Kaul R.,
RA   Brittnacher M., Miller S.I.;
RT   "Analysis of the genome of the Escherichia coli O157:H7 2006 spinach-
RT   associated outbreak isolate indicates candidate genes that may enhance
RT   virulence.";
RL   Infect. Immun. 77:3713-3721(2009).
CC   -!- CATALYTIC ACTIVITY: Acetyl-CoA + H(2)O + glyoxylate = (S)-malate +
CC       CoA.
CC   -!- PATHWAY: Carbohydrate metabolism; glyoxylate cycle; (S)-malate
CC       from isocitrate: step 2/2.
CC   -!- SIMILARITY: Belongs to the malate synthase family.
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DR   EMBL; CP001368; ACT74768.1; -; Genomic_DNA.
DR   RefSeq; YP_003080844.1; NC_013008.1.
DR   ProteinModelPortal; C6V1W7; -.
DR   STRING; 544404.ECSP_5083; -.
DR   EnsemblBacteria; ACT74768; ACT74768; ECSP_5083.
DR   GeneID; 8219649; -.
DR   KEGG; etw:ECSP_5083; -.
DR   PATRIC; 18394369; VBIEscCol9396_5143.
DR   eggNOG; COG2225; -.
DR   HOGENOM; HOG000238464; -.
DR   KO; K01638; -.
DR   OMA; GFDGTWV; -.
DR   ProtClustDB; PRK09255; -.
DR   UniPathway; UPA00703; UER00720.
DR   GO; GO:0004474; F:malate synthase activity; IEA:EC.
DR   GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   InterPro; IPR011076; Malate_synth-like.
DR   InterPro; IPR006252; Malate_synthA.
DR   InterPro; IPR001465; Malate_synthase.
DR   InterPro; IPR019830; Malate_synthase_CS.
DR   PANTHER; PTHR21631:SF1; PTHR21631:SF1; 1.
DR   Pfam; PF01274; Malate_synthase; 1.
DR   PIRSF; PIRSF001363; Malate_synth; 1.
DR   SUPFAM; SSF51645; Malat_synth_like; 1.
DR   TIGRFAMs; TIGR01344; malate_syn_A; 1.
DR   PROSITE; PS00510; MALATE_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Glyoxylate bypass; Transferase;
KW   Tricarboxylic acid cycle.
FT   ACT_SITE    166    166       Proton acceptor (By similarity).
FT   ACT_SITE    447    447       Proton donor (By similarity).
SQ   SEQUENCE   533 AA;  60276 MW;  EEB2FE43AED43D7E CRC64;
     MTEQATTTDE LAFIRPYGEQ EKQILTAEAV EFLTELVTHF TPQRNKLLAA RIQQQQDIDN
     GTLPDFISET ASIRDADWKI RGIPADLEDR RVEITGSVER KMVINALNAN VKVFMADFED
     SLAPDWNKVI DGQINLRDAV NGTISYTNEA GKIYQLKPNP AVLICRVRGL HLPEKHVTWR
     GEAIPGSLFD FALYFFHNYQ ALLAKGSGPY FYLPKTQSWQ EAAWWSEVFS YAEDRFNLPR
     GTIKATLLIE TLPAVFQMDE ILHALRDHIV GLNCGRWDYI FSYIKTLKNY PDRVLPDRQA
     VTMDKPFLNA YSRLLIKTCH KRGAFAMGGM AAFIPSKDEE HNNQVLNKVK ADKSLEANNG
     HDGTWIAHPG LADTAMAVFN DILGSRKNQL EVMREQDAPI TADQLLAPCD GERTEEGMRA
     NIRVAVQYIE AWISGNGCVP IYGLMEDAAT AEISRTSIWQ WIHHQKTLSN GKPVTKALFR
     QMLGEEMKVI ASELGEERFS QGRFDDAARL MEQITTSDEL IDFLTLPGYR LLA
//