ID C7BKH3_PHOAA Unreviewed; 272 AA.
AC C7BKH3;
DT 22-SEP-2009, integrated into UniProtKB/TrEMBL.
DT 22-SEP-2009, sequence version 1.
DT 01-MAY-2013, entry version 29.
DE RecName: Full=Ribosomal RNA small subunit methyltransferase A;
DE EC=2.1.1.182;
DE AltName: Full=16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase;
DE AltName: Full=16S rRNA dimethyladenosine transferase;
DE AltName: Full=16S rRNA dimethylase;
DE AltName: Full=S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase;
GN Name=rsmA; Synonyms=ksgA; OrderedLocusNames=PAU_00567;
OS Photorhabdus asymbiotica subsp. asymbiotica (strain ATCC 43949 /
OS 3105-77) (Xenorhabdus luminescens (strain 2)).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Photorhabdus.
OX NCBI_TaxID=553480;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43949 / 3105-77;
RX PubMed=19583835; DOI=10.1186/1471-2164-10-302;
RA Wilkinson P., Waterfield N.R., Crossman L., Corton C.,
RA Sanchez-Contreras M., Vlisidou I., Barron A., Bignell A., Clark L.,
RA Doggett J., Ormond D., Mayho M., Bason N., Smith F., Simmonds M.,
RA Arrowsmith C., Churcher C., Harris D., Thompson N.R., Quail M.,
RA Parkhill J., Ffrench-Constant R.H.;
RT "Comparative genomics of the emerging human pathogen Photorhabdus
RT asymbiotica with the insect pathogen Photorhabdus luminescens.";
RL BMC Genomics 10:302-302(2009).
CC -!- FUNCTION: Specifically dimethylates two adjacent adenosines (A1518
CC and A1519) in the loop of a conserved hairpin near the 3'-end of
CC 16S rRNA in the 30S particle. May play a critical role in
CC biogenesis of 30S subunits (By similarity).
CC -!- CATALYTIC ACTIVITY: 4 S-adenosyl-L-methionine +
CC adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-
CC homocysteine + N(6)-dimethyladenine(1518)/N(6)-
CC dimethyladenine(1519) in 16S rRNA.
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. rRNA
CC adenine N(6)-methyltransferase family. RsmA subfamily.
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DR EMBL; FM162591; CAQ82659.1; -; Genomic_DNA.
DR RefSeq; YP_003039404.1; NC_012962.1.
DR STRING; 291112.PAU_00567; -.
DR EnsemblBacteria; CAQ82659; CAQ82659; PAU_00567.
DR GeneID; 13861020; -.
DR KEGG; pay:PAU_00567; -.
DR PATRIC; 20494723; VBIPhoAsy71438_0651.
DR HOGENOM; HOG000227962; -.
DR KO; K02528; -.
DR OMA; PVPNVDS; -.
DR ProtClustDB; PRK00274; -.
DR BioCyc; PASY291112:GJQ0-584-MONOMER; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0052908; F:16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity; IEA:EC.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000179; F:rRNA (adenine-N6,N6-)-dimethyltransferase activity; IEA:InterPro.
DR GO; GO:0031167; P:rRNA methylation; IEA:GOC.
DR Gene3D; 1.10.8.100; -; 1.
DR HAMAP; MF_00607; 16SrRNA_methyltr_A; 1; -.
DR InterPro; IPR023165; rRNA_Ade_diMease-like.
DR InterPro; IPR020596; rRNA_Ade_Mease_Trfase_CS.
DR InterPro; IPR001737; rRNA_Ade_methylase_transferase.
DR InterPro; IPR020598; rRNA_Ade_methylase_Trfase_N.
DR InterPro; IPR011530; rRNA_adenine_dimethylase.
DR PANTHER; PTHR11727; PTHR11727; 1.
DR Pfam; PF00398; RrnaAD; 1.
DR SMART; SM00650; rADc; 1.
DR TIGRFAMs; TIGR00755; ksgA; 1.
DR PROSITE; PS01131; RRNA_A_DIMETH; 1.
PE 3: Inferred from homology;
KW Complete proteome; Cytoplasm; Methyltransferase; RNA-binding;
KW rRNA processing; S-adenosyl-L-methionine; Transferase.
FT BINDING 18 18 S-adenosyl-L-methionine; via carbonyl
FT oxygen (By similarity).
FT BINDING 20 20 S-adenosyl-L-methionine; via amide
FT nitrogen (By similarity).
FT BINDING 45 45 S-adenosyl-L-methionine; via carbonyl
FT oxygen (By similarity).
FT BINDING 66 66 S-adenosyl-L-methionine (By similarity).
FT BINDING 91 91 S-adenosyl-L-methionine (By similarity).
FT BINDING 113 113 S-adenosyl-L-methionine (By similarity).
SQ SEQUENCE 272 AA; 30596 MW; F680E56A249674FD CRC64;
MNNRVHQGHF ARKRFGQNFL TDQFVIDSIV AAINPQPGQA VLEIGPGLGA LTEPVGERMD
KMTVVELDRD LAARLQVHPQ LKDKLTIIQQ DAMTVNFGEL SQQRGLPLRV FGNLPYNIST
PLMFHLFSYT DAIADMHFML QKEVVNRLVA GPGSKAFGRL SVMAQYYCQV IPVLEVPPTA
FTPAPKVDSA VVRLIPHKSI PYPVKNIRML SRITTQAFNQ RRKTIRNSLG DLFTVEQLTE
FGIDPSTRAE NISVEQYCKM ANYLSEQPEM QL
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