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Database: UniProt/TrEMBL
Entry: C7CF80_METED
LinkDB: C7CF80_METED
Original site: C7CF80_METED 
ID   C7CF80_METED            Unreviewed;       250 AA.
AC   C7CF80;
DT   22-SEP-2009, integrated into UniProtKB/TrEMBL.
DT   22-SEP-2009, sequence version 1.
DT   09-DEC-2015, entry version 51.
DE   RecName: Full=tRNA pseudouridine synthase A {ECO:0000256|HAMAP-Rule:MF_00171};
DE            EC=5.4.99.12 {ECO:0000256|HAMAP-Rule:MF_00171};
DE   AltName: Full=tRNA pseudouridine(38-40) synthase {ECO:0000256|HAMAP-Rule:MF_00171};
DE   AltName: Full=tRNA pseudouridylate synthase I {ECO:0000256|HAMAP-Rule:MF_00171};
DE   AltName: Full=tRNA-uridine isomerase I {ECO:0000256|HAMAP-Rule:MF_00171};
GN   Name=truA {ECO:0000256|HAMAP-Rule:MF_00171,
GN   ECO:0000313|EMBL:CAX23904.1};
GN   OrderedLocusNames=METDI2302 {ECO:0000313|EMBL:CAX23904.1};
OS   Methylobacterium extorquens (strain DSM 5838 / DM4) (Methylobacterium
OS   dichloromethanicum (strain DM4)).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Methylobacteriaceae; Methylobacterium.
OX   NCBI_TaxID=661410 {ECO:0000313|EMBL:CAX23904.1, ECO:0000313|Proteomes:UP000008070};
RN   [1] {ECO:0000313|EMBL:CAX23904.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=DM4 {ECO:0000313|EMBL:CAX23904.1};
RA   Genoscope - CEA;
RL   Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Formation of pseudouridine at positions 38, 39 and 40 in
CC       the anticodon stem and loop of transfer RNAs. {ECO:0000256|HAMAP-
CC       Rule:MF_00171}.
CC   -!- CATALYTIC ACTIVITY: tRNA uridine(38-40) = tRNA pseudouridine(38-
CC       40). {ECO:0000256|HAMAP-Rule:MF_00171,
CC       ECO:0000256|RuleBase:RU003792, ECO:0000256|SAAS:SAAS00349402}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00171}.
CC   -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA
CC       family. {ECO:0000256|HAMAP-Rule:MF_00171,
CC       ECO:0000256|RuleBase:RU003792}.
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DR   EMBL; FP103042; CAX23904.1; -; Genomic_DNA.
DR   RefSeq; WP_015822214.1; NC_012988.1.
DR   EnsemblBacteria; CAX23904; CAX23904; METDI2302.
DR   KEGG; mdi:METDI2302; -.
DR   PATRIC; 22523215; VBIMetExt143287_2141.
DR   HOGENOM; HOG000248672; -.
DR   KO; K06173; -.
DR   OMA; YKLTIEY; -.
DR   OrthoDB; EOG6Z9B4R; -.
DR   BioCyc; MEXT661410:GJA1-2249-MONOMER; -.
DR   Proteomes; UP000008070; Chromosome.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-HAMAP.
DR   Gene3D; 3.30.70.580; -; 1.
DR   Gene3D; 3.30.70.660; -; 1.
DR   HAMAP; MF_00171; TruA; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom.
DR   InterPro; IPR001406; PsdUridine_synth_TruA.
DR   InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR   InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR   InterPro; IPR020094; PsdUridine_synth_TruA_N.
DR   PANTHER; PTHR11142; PTHR11142; 1.
DR   Pfam; PF01416; PseudoU_synth_1; 2.
DR   PIRSF; PIRSF001430; tRNA_psdUrid_synth; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00071; hisT_truA; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000008070};
KW   Isomerase {ECO:0000256|HAMAP-Rule:MF_00171,
KW   ECO:0000256|RuleBase:RU003792, ECO:0000256|SAAS:SAAS00023496,
KW   ECO:0000313|EMBL:CAX23904.1};
KW   tRNA processing {ECO:0000256|HAMAP-Rule:MF_00171,
KW   ECO:0000256|RuleBase:RU003792, ECO:0000256|SAAS:SAAS00023493}.
FT   DOMAIN        7    104       PseudoU_synth_1. {ECO:0000259|Pfam:
FT                                PF01416}.
FT   DOMAIN      144    249       PseudoU_synth_1. {ECO:0000259|Pfam:
FT                                PF01416}.
FT   ACT_SITE     52     52       Nucleophile. {ECO:0000256|HAMAP-Rule:
FT                                MF_00171, ECO:0000256|PIRSR:PIRSR001430-
FT                                1}.
FT   BINDING     111    111       Substrate. {ECO:0000256|HAMAP-Rule:
FT                                MF_00171, ECO:0000256|PIRSR:PIRSR001430-
FT                                2}.
SQ   SEQUENCE   250 AA;  27855 MW;  C96A801E0DC9D374 CRC64;
     MPRYKLVIEY DGAPFRGWQR QADDPTVQAA IETAVTRFSG ETARLTCAGR TDAGVHAIHQ
     VAHLDLAKDW RTDTVRDALN AHLRPQPVAI LSAEVVPQEF DARHSAIRRH YRYRILNRRS
     PAALTRAHVW QVPWPLDAEL MHRAAQRLLG HHDFSAFRAA ECQAKSPMRT LEQLDVTRVR
     MGLFEEIVIA TSARSFLHHQ VRAMAGTLML AGCNRLSADD VAEILATGAK HRCGPLAPAC
     GLTFVGVDYA
//
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