ID C7CF80_METED Unreviewed; 250 AA.
AC C7CF80;
DT 22-SEP-2009, integrated into UniProtKB/TrEMBL.
DT 22-SEP-2009, sequence version 1.
DT 01-MAY-2013, entry version 28.
DE RecName: Full=tRNA pseudouridine synthase A;
DE EC=5.4.99.12;
DE AltName: Full=tRNA pseudouridine(38-40) synthase;
DE AltName: Full=tRNA pseudouridylate synthase I;
DE AltName: Full=tRNA-uridine isomerase I;
GN Name=truA; OrderedLocusNames=METDI2302;
OS Methylobacterium extorquens (strain DSM 5838 / DM4) (Methylobacterium
OS dichloromethanicum (strain DM4)).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC Methylobacteriaceae; Methylobacterium.
OX NCBI_TaxID=661410;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 5838 / DM4;
RX PubMed=19440302; DOI=10.1371/journal.pone.0005584;
RA Vuilleumier S., Chistoserdova L., Lee M.C., Bringel F., Lajus A.,
RA Zhou Y., Gourion B., Barbe V., Chang J., Cruveiller S., Dossat C.,
RA Gillett W., Gruffaz C., Haugen E., Hourcade E., Levy R., Mangenot S.,
RA Muller E., Nadalig T., Pagni M., Penny C., Peyraud R., Robinson D.G.,
RA Roche D., Rouy Z., Saenampechek C., Salvignol G., Vallenet D., Wu Z.,
RA Marx C.J., Vorholt J.A., Olson M.V., Kaul R., Weissenbach J.,
RA Medigue C., Lidstrom M.E.;
RT "Methylobacterium genome sequences: a reference blueprint to
RT investigate microbial metabolism of C1 compounds from natural and
RT industrial sources.";
RL PLoS ONE 4:E5584-E5584(2009).
CC -!- FUNCTION: Formation of pseudouridine at positions 38, 39 and 40 in
CC the anticodon stem and loop of transfer RNAs (By similarity).
CC -!- CATALYTIC ACTIVITY: tRNA uridine = tRNA pseudouridine.
CC -!- CATALYTIC ACTIVITY: tRNA uridine(38-40) = tRNA pseudouridine(38-
CC 40).
CC -!- SUBUNIT: Homodimer (By similarity).
CC -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA
CC family.
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DR EMBL; FP103042; CAX23904.1; -; Genomic_DNA.
DR RefSeq; YP_003067850.1; NC_012988.1.
DR STRING; 661410.METDI2302; -.
DR EnsemblBacteria; CAX23904; CAX23904; METDI2302.
DR GeneID; 8195273; -.
DR KEGG; mdi:METDI2302; -.
DR PATRIC; 22523215; VBIMetExt143287_2141.
DR eggNOG; COG0101; -.
DR HOGENOM; HOG000248672; -.
DR KO; K06173; -.
DR OMA; RHYRYRI; -.
DR ProtClustDB; PRK00021; -.
DR BioCyc; MEXT661410:GJA1-2249-MONOMER; -.
DR GO; GO:0009982; F:pseudouridine synthase activity; IEA:HAMAP.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:HAMAP.
DR Gene3D; 3.30.70.580; -; 1.
DR Gene3D; 3.30.70.660; -; 1.
DR HAMAP; MF_00171; TruA; 1; -.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom.
DR InterPro; IPR001406; PsdUridine_synth_TruA.
DR InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR InterPro; IPR020094; PsdUridine_synth_TruA_N.
DR PANTHER; PTHR11142; PTHR11142; 1.
DR Pfam; PF01416; PseudoU_synth_1; 2.
DR PIRSF; PIRSF001430; tRNA_psdUrid_synth; 1.
DR SUPFAM; SSF55120; PsdUridine_synth_cat_dom; 1.
DR TIGRFAMs; TIGR00071; hisT_truA; 1.
PE 3: Inferred from homology;
KW Complete proteome; Isomerase; tRNA processing.
FT ACT_SITE 52 52 Nucleophile (By similarity).
FT BINDING 111 111 Substrate (By similarity).
SQ SEQUENCE 250 AA; 27855 MW; C96A801E0DC9D374 CRC64;
MPRYKLVIEY DGAPFRGWQR QADDPTVQAA IETAVTRFSG ETARLTCAGR TDAGVHAIHQ
VAHLDLAKDW RTDTVRDALN AHLRPQPVAI LSAEVVPQEF DARHSAIRRH YRYRILNRRS
PAALTRAHVW QVPWPLDAEL MHRAAQRLLG HHDFSAFRAA ECQAKSPMRT LEQLDVTRVR
MGLFEEIVIA TSARSFLHHQ VRAMAGTLML AGCNRLSADD VAEILATGAK HRCGPLAPAC
GLTFVGVDYA
//
