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Database: UniProt/TrEMBL
Entry: C7CF80_METED
LinkDB: C7CF80_METED
Original site: C7CF80_METED 
ID   C7CF80_METED            Unreviewed;       250 AA.
AC   C7CF80;
DT   22-SEP-2009, integrated into UniProtKB/TrEMBL.
DT   22-SEP-2009, sequence version 1.
DT   09-JUL-2014, entry version 36.
DE   RecName: Full=tRNA pseudouridine synthase A;
DE            EC=5.4.99.12;
DE   AltName: Full=tRNA pseudouridine(38-40) synthase;
DE   AltName: Full=tRNA pseudouridylate synthase I;
DE   AltName: Full=tRNA-uridine isomerase I;
GN   Name=truA; OrderedLocusNames=METDI2302;
OS   Methylobacterium extorquens (strain DSM 5838 / DM4) (Methylobacterium
OS   dichloromethanicum (strain DM4)).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Methylobacteriaceae; Methylobacterium.
OX   NCBI_TaxID=661410;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 5838 / DM4;
RX   PubMed=19440302; DOI=10.1371/journal.pone.0005584;
RA   Vuilleumier S., Chistoserdova L., Lee M.-C., Bringel F., Lajus A.,
RA   Zhou Y., Gourion B., Barbe V., Chang J., Cruveiller S., Dossat C.,
RA   Gillett W., Gruffaz C., Haugen E., Hourcade E., Levy R., Mangenot S.,
RA   Muller E., Nadalig T., Pagni M., Penny C., Peyraud R., Robinson D.G.,
RA   Roche D., Rouy Z., Saenampechek C., Salvignol G., Vallenet D., Wu Z.,
RA   Marx C.J., Vorholt J.A., Olson M.V., Kaul R., Weissenbach J.,
RA   Medigue C., Lidstrom M.E.;
RT   "Methylobacterium genome sequences: a reference blueprint to
RT   investigate microbial metabolism of C1 compounds from natural and
RT   industrial sources.";
RL   PLoS ONE 4:E5584-E5584(2009).
CC   -!- FUNCTION: Formation of pseudouridine at positions 38, 39 and 40 in
CC       the anticodon stem and loop of transfer RNAs (By similarity).
CC   -!- CATALYTIC ACTIVITY: tRNA uridine(38-40) = tRNA pseudouridine(38-
CC       40).
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA
CC       family.
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DR   EMBL; FP103042; CAX23904.1; -; Genomic_DNA.
DR   RefSeq; YP_003067850.1; NC_012988.1.
DR   STRING; 661410.METDI2302; -.
DR   EnsemblBacteria; CAX23904; CAX23904; METDI2302.
DR   GeneID; 8195273; -.
DR   KEGG; mdi:METDI2302; -.
DR   PATRIC; 22523215; VBIMetExt143287_2141.
DR   eggNOG; COG0101; -.
DR   HOGENOM; HOG000248672; -.
DR   KO; K06173; -.
DR   OMA; YLTKVEY; -.
DR   OrthoDB; EOG6Z9B4R; -.
DR   BioCyc; MEXT661410:GJA1-2249-MONOMER; -.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-HAMAP.
DR   Gene3D; 3.30.70.580; -; 1.
DR   Gene3D; 3.30.70.660; -; 1.
DR   HAMAP; MF_00171; TruA; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom.
DR   InterPro; IPR001406; PsdUridine_synth_TruA.
DR   InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR   InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR   InterPro; IPR020094; PsdUridine_synth_TruA_N.
DR   PANTHER; PTHR11142; PTHR11142; 1.
DR   Pfam; PF01416; PseudoU_synth_1; 2.
DR   PIRSF; PIRSF001430; tRNA_psdUrid_synth; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00071; hisT_truA; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Isomerase; tRNA processing.
FT   ACT_SITE     52     52       Nucleophile (By similarity){EA3,EA7}.
FT   BINDING     111    111       Substrate (By similarity){EA3,EA6}.
SQ   SEQUENCE   250 AA;  27855 MW;  C96A801E0DC9D374 CRC64;
     MPRYKLVIEY DGAPFRGWQR QADDPTVQAA IETAVTRFSG ETARLTCAGR TDAGVHAIHQ
     VAHLDLAKDW RTDTVRDALN AHLRPQPVAI LSAEVVPQEF DARHSAIRRH YRYRILNRRS
     PAALTRAHVW QVPWPLDAEL MHRAAQRLLG HHDFSAFRAA ECQAKSPMRT LEQLDVTRVR
     MGLFEEIVIA TSARSFLHHQ VRAMAGTLML AGCNRLSADD VAEILATGAK HRCGPLAPAC
     GLTFVGVDYA
//
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