ID C7NUJ1_HALUD Unreviewed; 489 AA.
AC C7NUJ1;
DT 13-OCT-2009, integrated into UniProtKB/TrEMBL.
DT 13-OCT-2009, sequence version 1.
DT 01-MAY-2013, entry version 23.
DE RecName: Full=tRNA-guanine(15) transglycosylase;
DE EC=2.4.2.48;
DE AltName: Full=7-cyano-7-deazaguanine tRNA-ribosyltransferase;
DE AltName: Full=Archaeal tRNA-guanine transglycosylase;
GN Name=tgtA; OrderedLocusNames=Huta_2181;
OS Halorhabdus utahensis (strain DSM 12940 / JCM 11049 / AX-2).
OC Archaea; Euryarchaeota; Halobacteria; Halobacteriales;
OC Halobacteriaceae; Halorhabdus.
OX NCBI_TaxID=519442;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 12940 / JCM 11049 / AX-2;
RX DOI=10.4056/sigs.31864;
RA Anderson I., Tindall B.J., Pomrenke H., Goker M., Lapidus A.,
RA Nolan M., Copeland A., Glavina Del Rio T., Chen F., Tice H.,
RA Cheng J.F., Lucas S., Chertkov O., Bruce D., Brettin T., Detter J.C.,
RA Han C., Goodwin L., Land M., Hauser L., Chang Y.J., Jeffries C.D.,
RA Pitluck S., Pati A., Mavromatis K., Ivanova N., Ovchinnikova G.,
RA Chen A., Palaniappan K., Chain P., Rohde M., Bristow J., Eisen J.A.,
RA Markowitz V., Hugenholtz P., Kyrpides N.C., Klenk H.P.;
RT "Complete genome sequence of Halorhabdus utahensis type strain (AX-
RT 2).";
RL Stand. Genomic Sci. 1:218-225(2009).
CC -!- FUNCTION: Exchanges the guanine residue with 7-cyano-7-
CC deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D-
CC loop) of archaeal tRNAs (By similarity).
CC -!- CATALYTIC ACTIVITY: Guanine(15) in tRNA + 7-cyano-7-carbaguanine =
CC 7-cyano-7-carbaguanine(15) in tRNA + guanine.
CC -!- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
CC -!- PATHWAY: tRNA modification; archaeosine-tRNA biosynthesis.
CC -!- SIMILARITY: Belongs to the archaeosine tRNA-ribosyltransferase
CC family.
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DR EMBL; CP001687; ACV12348.1; -; Genomic_DNA.
DR RefSeq; YP_003131081.1; NC_013158.1.
DR STRING; 519442.Huta_2181; -.
DR EnsemblBacteria; ACV12348; ACV12348; Huta_2181.
DR GeneID; 8384475; -.
DR KEGG; hut:Huta_2181; -.
DR eggNOG; COG0343; -.
DR HOGENOM; HOG000231009; -.
DR KO; K00773; -.
DR OMA; VETPTVM; -.
DR ProtClustDB; PRK13533; -.
DR BioCyc; HUTA519442:GH3V-2216-MONOMER; -.
DR UniPathway; UPA00393; -.
DR GO; GO:0008479; F:queuine tRNA-ribosyltransferase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0008616; P:queuosine biosynthetic process; IEA:InterPro.
DR Gene3D; 3.20.20.105; -; 1.
DR HAMAP; MF_01634; TgtA_arch; 1; -.
DR InterPro; IPR004804; Arc_tRNA_RibTrfase.
DR InterPro; IPR002616; tRNA_ribo_trans.
DR PANTHER; PTHR11962; PTHR11962; 1.
DR Pfam; PF01702; TGT; 1.
DR SUPFAM; SSF51713; tRNA_ribo_trans; 1.
DR TIGRFAMs; TIGR00432; arcsn_tRNA_tgt; 1.
DR TIGRFAMs; TIGR00449; tgt_general; 1.
PE 3: Inferred from homology;
KW Complete proteome; Glycosyltransferase; Metal-binding; Transferase;
KW tRNA processing; Zinc.
FT ACT_SITE 92 92 Nucleophile (By similarity).
FT METAL 278 278 Zinc (By similarity).
FT METAL 280 280 Zinc (By similarity).
FT METAL 283 283 Zinc (By similarity).
SQ SEQUENCE 489 AA; 53566 MW; 11E82DDA4BF5F438 CRC64;
MRDHFELQAY DAMGRLGELT VPRADVTVET PALLPVINPH HETIDPGRLA EFGAEILITN
SYVIYGSDEV REPALEDGLH DLLDFDGAIM TDSGSFQLAE YGEIDVNTTE ILEFQREIGS
DIATPVDIPT PPDADREQVG REMETTQARL ETAADFDAGE MLLNAPVQGA TYPDLREQAA
RDAYATGLDV FPIGAVVPLL NDYRYGEMVD AVAGAKRGLG EDAPVHLFGA GHPMMFALAV
AAGCDLFDSA AYALYARDGR YLTVRGTEHL DELEHFPCEC PVCSARTPAE LRDRPTHERT
KLLAEHNLHV SFGEMRRIKQ AIRQGNLLEL VETRARAHPT MLDGYRALLD HAEQLERTDP
ASKDAFFYLS GESAARPEVR RHHDRLARLS PPADLLLATD PDAHAGADAD VWAVEPPFGP
VPRGLDQTYP LTAELPDRLD DRAYRAAARG VNRLAETSDA AITLVDDGWP ASALDLLAEG
VDIDTTVEE
//
