ID D0R4F1_LACJF Unreviewed; 250 AA.
AC D0R4F1;
DT 15-DEC-2009, integrated into UniProtKB/TrEMBL.
DT 15-DEC-2009, sequence version 1.
DT 01-MAY-2013, entry version 25.
DE RecName: Full=Ribonuclease HII;
DE Short=RNase HII;
DE EC=3.1.26.4;
GN Name=rnhB; OrderedLocusNames=FI9785_1100;
OS Lactobacillus johnsonii (strain FI9785).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lactobacillus.
OX NCBI_TaxID=633699;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FI9785;
RX PubMed=19767436; DOI=10.1128/JB.01182-09;
RA Wegmann U., Overweg K., Horn N., Goesmann A., Narbad A., Gasson M.J.,
RA Shearman C.;
RT "Complete genome sequence of Lactobacillus johnsonii FI9785, a
RT competitive exclusion agent against pathogens in poultry.";
RL J. Bacteriol. 191:7142-7143(2009).
CC -!- FUNCTION: Endonuclease that specifically degrades the RNA of RNA-
CC DNA hybrids (By similarity).
CC -!- CATALYTIC ACTIVITY: Endonucleolytic cleavage to 5'-
CC phosphomonoester.
CC -!- COFACTOR: Manganese or magnesium. Binds 1 divalent metal ion per
CC monomer in the absence of substrate. May bind a second metal ion
CC after substrate binding (By similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- SIMILARITY: Belongs to the RNase HII family.
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DR EMBL; FN298497; CAX66964.1; -; Genomic_DNA.
DR RefSeq; YP_003293231.1; NC_013504.1.
DR STRING; 633699.FI9785_1100; -.
DR EnsemblBacteria; CAX66964; CAX66964; FI9785_1100.
DR GeneID; 8570867; -.
DR KEGG; ljf:FI9785_1100; -.
DR PATRIC; 32246789; VBILacJoh137736_1025.
DR eggNOG; COG0164; -.
DR HOGENOM; HOG000100288; -.
DR KO; K03470; -.
DR BioCyc; LJOH633699:GJ95-1033-MONOMER; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0030145; F:manganese ion binding; IEA:HAMAP.
DR GO; GO:0004523; F:ribonuclease H activity; IEA:HAMAP.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0090305; P:nucleic acid phosphodiester bond hydrolysis; IEA:GOC.
DR GO; GO:0006401; P:RNA catabolic process; IEA:HAMAP.
DR HAMAP; MF_00052_B; RNase_HII_B; 1; -.
DR InterPro; IPR022898; RNase_HII.
DR InterPro; IPR001352; RNase_HII/HIII.
DR InterPro; IPR024567; RNase_HII/HIII_dom.
DR InterPro; IPR012337; RNaseH-like_dom.
DR PANTHER; PTHR10954; PTHR10954; 1.
DR Pfam; PF01351; RNase_HII; 1.
DR SUPFAM; SSF53098; RNaseH_fold; 1.
PE 3: Inferred from homology;
KW Complete proteome; Cytoplasm; Endonuclease; Hydrolase; Manganese;
KW Metal-binding; Nuclease.
FT METAL 72 72 Divalent metal cation (By similarity).
FT METAL 73 73 Divalent metal cation (By similarity).
FT METAL 164 164 Divalent metal cation (By similarity).
SQ SEQUENCE 250 AA; 27948 MW; 9D8A0E00E8901CE9 CRC64;
MTITEIKNLL QGEVSKEQLE ELKADERKGV QKLLISYEKR QAKYAKALAQ FQSRFSYEKE
FWQKDQLVAG VDEVGRGPLA GPVVTAAVIL PHDFDLIDVN DSKKLSPKKR QALFPKILEK
AVSVSVGLAN NDLIDQINIY EADRVAMAHA VQGLKVKPDA LLVDAMNVPL NIPQVKLIHG
DAKSNSIAAA SIVAKVFRDN LMDAYGELYP EYDFKHNAGY GTREHMEALK KYGPTPIHRR
SFAPVSEYEK
//
