ID D3S8I2_METSF Unreviewed; 166 AA.
AC D3S8I2;
DT 20-APR-2010, integrated into UniProtKB/TrEMBL.
DT 20-APR-2010, sequence version 1.
DT 29-MAY-2013, entry version 25.
DE RecName: Full=3-isopropylmalate dehydratase small subunit;
DE EC=4.2.1.33;
DE AltName: Full=Alpha-IPM isomerase;
DE AltName: Full=Isopropylmalate isomerase;
GN Name=leuD; OrderedLocusNames=MFS40622_0654;
OS Methanocaldococcus sp. (strain FS406-22).
OC Archaea; Euryarchaeota; Methanococci; Methanococcales;
OC Methanocaldococcaceae; Methanocaldococcus.
OX NCBI_TaxID=644281;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FS406-22;
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L.,
RA Pitluck S., Teshima H., Detter J.C., Han C., Tapia R., Larimer F.,
RA Land M., Hauser L., Kyrpides N., Mikhailova N., Sieprawska-Lupa M.,
RA Leigh J., Whitman W.B., Woyke T.;
RT "Complete sequence of chromosome of Methanocaldococcus sp. FS406-22.";
RL Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the isomerization between 2-isopropylmalate
CC and 3-isopropylmalate, via the formation of 2-isopropylmaleate (By
CC similarity).
CC -!- CATALYTIC ACTIVITY: (2R,3S)-3-isopropylmalate = (2S)-2-
CC isopropylmalate.
CC -!- PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-
CC leucine from 3-methyl-2-oxobutanoate: step 2/4.
CC -!- SUBUNIT: Heterodimer of LeuC and LeuD (By similarity).
CC -!- SIMILARITY: Belongs to the LeuD family. LeuD type 2 subfamily.
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DR EMBL; CP001901; ADC69342.1; -; Genomic_DNA.
DR RefSeq; YP_003458078.1; NC_013887.1.
DR EnsemblBacteria; ADC69342; ADC69342; MFS40622_0654.
DR GeneID; 8804495; -.
DR KEGG; mfs:MFS40622_0654; -.
DR HOGENOM; HOG000222940; -.
DR KO; K01704; -.
DR OMA; VHENDIV; -.
DR BioCyc; MSP644281:GI4H-661-MONOMER; -.
DR UniPathway; UPA00048; UER00071.
DR GO; GO:0009316; C:3-isopropylmalate dehydratase complex; IEA:InterPro.
DR GO; GO:0003861; F:3-isopropylmalate dehydratase activity; IEA:HAMAP.
DR GO; GO:0009098; P:leucine biosynthetic process; IEA:HAMAP.
DR Gene3D; 3.20.19.10; -; 1.
DR HAMAP; MF_01032; LeuD_type2; 1; -.
DR InterPro; IPR015937; Acoase/IPM_deHydtase.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR InterPro; IPR011827; IsopropMal_deHydtase_ssu.
DR PANTHER; PTHR11670; PTHR11670; 1.
DR Pfam; PF00694; Aconitase_C; 1.
DR SUPFAM; SSF52016; Aconitase/3IPM_dehydase_swvl; 1.
DR TIGRFAMs; TIGR02087; LEUD_arch; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis; Branched-chain amino acid biosynthesis;
KW Complete proteome; Leucine biosynthesis; Lyase.
SQ SEQUENCE 166 AA; 18120 MW; B83F8806225D5FEA CRC64;
MQSVIKGRVW KFGDNVDTDA ILPARYLVYT KPEELAQFVM TGADPDFPKK VKPGDIIVGG
KNFGCGSSRE HAPLGLKGAG ISCVIAESFA RIFYRNAINV GLPLIECKGI SEKVNEGDEL
EVNLETGEIK NLTTGEVLKG QKLPEFMMEI LEAGGLMPYL KKKMKA
//
