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Database: UniProt/TrEMBL
Entry: D3USK5_LISSS
LinkDB: D3USK5_LISSS
Original site: D3USK5_LISSS 
ID   D3USK5_LISSS            Unreviewed;       248 AA.
AC   D3USK5;
DT   20-APR-2010, integrated into UniProtKB/TrEMBL.
DT   20-APR-2010, sequence version 1.
DT   26-NOV-2014, entry version 32.
DE   RecName: Full=tRNA pseudouridine synthase A {ECO:0000256|HAMAP-Rule:MF_00171};
DE            EC=5.4.99.12 {ECO:0000256|HAMAP-Rule:MF_00171};
DE   AltName: Full=tRNA pseudouridine(38-40) synthase {ECO:0000256|HAMAP-Rule:MF_00171};
DE   AltName: Full=tRNA pseudouridylate synthase I {ECO:0000256|HAMAP-Rule:MF_00171};
DE   AltName: Full=tRNA-uridine isomerase I {ECO:0000256|HAMAP-Rule:MF_00171};
GN   Name=truA {ECO:0000256|HAMAP-Rule:MF_00171,
GN   ECO:0000313|EMBL:CBH28654.1};
GN   OrderedLocusNames=lse_2503 {ECO:0000313|EMBL:CBH28654.1};
OS   Listeria seeligeri serovar 1/2b (strain ATCC 35967 / DSM 20751 / CIP
OS   100100 / SLCC 3954).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria.
OX   NCBI_TaxID=683837 {ECO:0000313|EMBL:CBH28654.1, ECO:0000313|Proteomes:UP000002044};
RN   [1] {ECO:0000313|EMBL:CBH28654.1, ECO:0000313|Proteomes:UP000002044}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35967 / DSM 20751 / CIP 100100 / SLCC 3954
RC   {ECO:0000313|Proteomes:UP000002044};
RX   PubMed=20061480; DOI=10.1128/JB.01415-09;
RA   Steinweg C., Kuenne C.T., Billion A., Mraheil M.A., Domann E.,
RA   Ghai R., Barbuddhe S.B., Karst U., Goesmann A., Puhler A.,
RA   Weisshaar B., Wehland J., Lampidis R., Kreft J., Goebel W.,
RA   Chakraborty T., Hain T.;
RT   "Complete genome sequence of Listeria seeligeri, a nonpathogenic
RT   member of the genus Listeria.";
RL   J. Bacteriol. 192:1473-1474(2010).
CC   -!- FUNCTION: Formation of pseudouridine at positions 38, 39 and 40 in
CC       the anticodon stem and loop of transfer RNAs. {ECO:0000256|HAMAP-
CC       Rule:MF_00171, ECO:0000256|SAAS:SAAS00023494}.
CC   -!- CATALYTIC ACTIVITY: tRNA uridine(38-40) = tRNA pseudouridine(38-
CC       40). {ECO:0000256|HAMAP-Rule:MF_00171,
CC       ECO:0000256|RuleBase:RU003792, ECO:0000256|SAAS:SAAS00023491}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00171}.
CC   -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA
CC       family. {ECO:0000256|HAMAP-Rule:MF_00171,
CC       ECO:0000256|RuleBase:RU003792}.
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DR   EMBL; FN557490; CBH28654.1; -; Genomic_DNA.
DR   RefSeq; WP_012986488.1; NC_013891.1.
DR   RefSeq; YP_003465736.1; NC_013891.1.
DR   EnsemblBacteria; CBH28654; CBH28654; lse_2503.
DR   GeneID; 9081257; -.
DR   KEGG; lsg:lse_2503; -.
DR   PATRIC; 32265038; VBILisSee138575_2506.
DR   HOGENOM; HOG000248673; -.
DR   KO; K06173; -.
DR   OMA; YAMNSAL; -.
DR   BioCyc; LSEE683837:GI10-2587-MONOMER; -.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-HAMAP.
DR   Gene3D; 3.30.70.580; -; 1.
DR   Gene3D; 3.30.70.660; -; 1.
DR   HAMAP; MF_00171; TruA; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom.
DR   InterPro; IPR001406; PsdUridine_synth_TruA.
DR   InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR   InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR   InterPro; IPR020094; PsdUridine_synth_TruA_N.
DR   PANTHER; PTHR11142; PTHR11142; 1.
DR   Pfam; PF01416; PseudoU_synth_1; 2.
DR   PIRSF; PIRSF001430; tRNA_psdUrid_synth; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00071; hisT_truA; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000002044};
KW   Isomerase {ECO:0000256|HAMAP-Rule:MF_00171,
KW   ECO:0000256|RuleBase:RU003792}; Lyase {ECO:0000313|EMBL:CBH28654.1};
KW   tRNA processing {ECO:0000256|HAMAP-Rule:MF_00171,
KW   ECO:0000256|RuleBase:RU003792}.
FT   ACT_SITE     53     53       Nucleophile. {ECO:0000256|HAMAP-Rule:
FT                                MF_00171, ECO:0000256|PIRSR:PIRSR001430-
FT                                1}.
FT   BINDING     111    111       Substrate. {ECO:0000256|HAMAP-Rule:
FT                                MF_00171, ECO:0000256|PIRSR:PIRSR001430-
FT                                2}.
SQ   SEQUENCE   248 AA;  28366 MW;  5DFF1F1CB1E43AD1 CRC64;
     MTRYKAIISY DGSGFYGYQV QPDTRTVQAE IEKALKKMHK GKSVRVTASG RTDTGVHAKG
     QVIHFDSELD ISAEKFQKAL QVMTPFDISF LIVEEAPADF HARFGTVGKE YRYVVKRTKV
     FDPFSRNFAL HYPYELNLEK MKLASERLVG EHDFTSFCSA RTERDSKVRT LYSIDFYEEE
     DDTLVIAFQG NGFLYNMVRI LTGTLLDAGQ GRISPDDITK ALLARDRQKL ISKTAPPQGL
     YLWRVDYE
//
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