ID D3USK5_LISSS Unreviewed; 248 AA.
AC D3USK5;
DT 20-APR-2010, integrated into UniProtKB/TrEMBL.
DT 20-APR-2010, sequence version 1.
DT 29-MAY-2013, entry version 25.
DE RecName: Full=tRNA pseudouridine synthase A;
DE EC=5.4.99.12;
DE AltName: Full=tRNA pseudouridine(38-40) synthase;
DE AltName: Full=tRNA pseudouridylate synthase I;
DE AltName: Full=tRNA-uridine isomerase I;
GN Name=truA; OrderedLocusNames=lse_2503;
OS Listeria seeligeri serovar 1/2b (strain ATCC 35967 / DSM 20751 / CIP
OS 100100 / SLCC 3954).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria.
OX NCBI_TaxID=683837;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35967 / DSM 20751 / CIP 100100 / SLCC 3954;
RX PubMed=20061480; DOI=10.1128/JB.01415-09;
RA Steinweg C., Kuenne C.T., Billion A., Mraheil M.A., Domann E.,
RA Ghai R., Barbuddhe S.B., Karst U., Goesmann A., Puhler A.,
RA Weisshaar B., Wehland J., Lampidis R., Kreft J., Goebel W.,
RA Chakraborty T., Hain T.;
RT "Complete genome sequence of Listeria seeligeri, a nonpathogenic
RT member of the genus Listeria.";
RL J. Bacteriol. 192:1473-1474(2010).
CC -!- FUNCTION: Formation of pseudouridine at positions 38, 39 and 40 in
CC the anticodon stem and loop of transfer RNAs (By similarity).
CC -!- CATALYTIC ACTIVITY: tRNA uridine = tRNA pseudouridine.
CC -!- CATALYTIC ACTIVITY: tRNA uridine(38-40) = tRNA pseudouridine(38-
CC 40).
CC -!- SUBUNIT: Homodimer (By similarity).
CC -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA
CC family.
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DR EMBL; FN557490; CBH28654.1; -; Genomic_DNA.
DR RefSeq; YP_003465736.1; NC_013891.1.
DR EnsemblBacteria; CBH28654; CBH28654; lse_2503.
DR GeneID; 9081257; -.
DR KEGG; lsg:lse_2503; -.
DR PATRIC; 32265038; VBILisSee138575_2506.
DR HOGENOM; HOG000248673; -.
DR KO; K06173; -.
DR BioCyc; LSEE683837:GI10-2587-MONOMER; -.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0009982; F:pseudouridine synthase activity; IEA:HAMAP.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:HAMAP.
DR Gene3D; 3.30.70.580; -; 1.
DR Gene3D; 3.30.70.660; -; 1.
DR HAMAP; MF_00171; TruA; 1; -.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom.
DR InterPro; IPR001406; PsdUridine_synth_TruA.
DR InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR InterPro; IPR020094; PsdUridine_synth_TruA_N.
DR PANTHER; PTHR11142; PTHR11142; 1.
DR Pfam; PF01416; PseudoU_synth_1; 2.
DR PIRSF; PIRSF001430; tRNA_psdUrid_synth; 1.
DR SUPFAM; SSF55120; PsdUridine_synth_cat_dom; 1.
DR TIGRFAMs; TIGR00071; hisT_truA; 1.
PE 3: Inferred from homology;
KW Complete proteome; Isomerase; Lyase; tRNA processing.
FT ACT_SITE 53 53 Nucleophile (By similarity).
FT BINDING 111 111 Substrate (By similarity).
SQ SEQUENCE 248 AA; 28366 MW; 5DFF1F1CB1E43AD1 CRC64;
MTRYKAIISY DGSGFYGYQV QPDTRTVQAE IEKALKKMHK GKSVRVTASG RTDTGVHAKG
QVIHFDSELD ISAEKFQKAL QVMTPFDISF LIVEEAPADF HARFGTVGKE YRYVVKRTKV
FDPFSRNFAL HYPYELNLEK MKLASERLVG EHDFTSFCSA RTERDSKVRT LYSIDFYEEE
DDTLVIAFQG NGFLYNMVRI LTGTLLDAGQ GRISPDDITK ALLARDRQKL ISKTAPPQGL
YLWRVDYE
//
