GenomeNet

Database: UniProt/TrEMBL
Entry: D5D6K4_ECOKI
LinkDB: D5D6K4_ECOKI
Original site: D5D6K4_ECOKI 
ID   D5D6K4_ECOKI            Unreviewed;       533 AA.
AC   D5D6K4;
DT   15-JUN-2010, integrated into UniProtKB/TrEMBL.
DT   15-JUN-2010, sequence version 1.
DT   01-OCT-2014, entry version 28.
DE   RecName: Full=Malate synthase {ECO:0000256|RuleBase:RU000555};
DE            EC=2.3.3.9 {ECO:0000256|RuleBase:RU000555};
GN   Name=aceB {ECO:0000313|EMBL:ADE92247.1};
GN   OrderedLocusNames=ECOK1_4491 {ECO:0000313|EMBL:ADE92247.1};
OS   Escherichia coli O18:K1:H7 (strain IHE3034 / ExPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=714962 {ECO:0000313|EMBL:ADE92247.1, ECO:0000313|Proteomes:UP000002364};
RN   [1] {ECO:0000313|EMBL:ADE92247.1, ECO:0000313|Proteomes:UP000002364}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IHE3034 / ExPEC {ECO:0000313|Proteomes:UP000002364};
RX   PubMed=20439758; DOI=10.1073/pnas.0915077107;
RA   Moriel D.G., Bertoldi I., Spagnuolo A., Marchi S., Rosini R.,
RA   Nesta B., Pastorello I., Corea V.A., Torricelli G., Cartocci E.,
RA   Savino S., Scarselli M., Dobrindt U., Hacker J., Tettelin H.,
RA   Tallon L.J., Sullivan S., Wieler L.H., Ewers C., Pickard D.,
RA   Dougan G., Fontana M.R., Rappuoli R., Pizza M., Serino L.;
RT   "Identification of protective and broadly conserved vaccine antigens
RT   from the genome of extraintestinal pathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 107:9072-9077(2010).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=IHE3034;
RA   Moriel D.G., Bertoldi I., Spagnuolo A., Marchi S., Rosini R.,
RA   Nesta B., Pastorello I., Mariani Corea V.A., Torricelli G.,
RA   Cartocci E., Savino S., Scarselli M., Dobrindt U., Hacker J.,
RA   Tettelin H., Wieler L.H., Ewers C., Picard D., Dougan G.,
RA   Fontana M.R., Rappuoli R., Pizza M., Serino L.;
RT   "Identification of protective and broadly conserved vaccine antigens
RT   from the genome of Extraintestinal Pathogenic Escherichia coli
RT   (ExPEC).";
RL   Proc. Natl. Acad. Sci. U.S.A. 0:0-0(2010).
CC   -!- CATALYTIC ACTIVITY: Acetyl-CoA + H(2)O + glyoxylate = (S)-malate +
CC       CoA. {ECO:0000256|RuleBase:RU000555}.
CC   -!- PATHWAY: Carbohydrate metabolism; glyoxylate cycle; (S)-malate
CC       from isocitrate: step 2/2. {ECO:0000256|RuleBase:RU000555}.
CC   -!- SIMILARITY: Belongs to the malate synthase family.
CC       {ECO:0000256|RuleBase:RU000555}.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; CP001969; ADE92247.1; -; Genomic_DNA.
DR   RefSeq; YP_006103553.1; NC_017628.1.
DR   EnsemblBacteria; ADE92247; ADE92247; ECOK1_4491.
DR   GeneID; 12693057; -.
DR   KEGG; eih:ECOK1_4491; -.
DR   PATRIC; 36710529; VBIEscCol148715_4518.
DR   HOGENOM; HOG000238464; -.
DR   KO; K01638; -.
DR   BioCyc; ECOL714962:GI9T-4489-MONOMER; -.
DR   UniPathway; UPA00703; UER00720.
DR   GO; GO:0004474; F:malate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   InterPro; IPR011076; Malate_synth-like.
DR   InterPro; IPR006252; Malate_synthA.
DR   InterPro; IPR001465; Malate_synthase.
DR   InterPro; IPR019830; Malate_synthase_CS.
DR   Pfam; PF01274; Malate_synthase; 1.
DR   PIRSF; PIRSF001363; Malate_synth; 1.
DR   SUPFAM; SSF51645; SSF51645; 1.
DR   TIGRFAMs; TIGR01344; malate_syn_A; 1.
DR   PROSITE; PS00510; MALATE_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Acyltransferase {ECO:0000313|EMBL:ADE92247.1};
KW   Complete proteome {ECO:0000313|Proteomes:UP000002364};
KW   Glyoxylate bypass {ECO:0000256|RuleBase:RU000555};
KW   Transferase {ECO:0000256|RuleBase:RU000555,
KW   ECO:0000313|EMBL:ADE92247.1};
KW   Tricarboxylic acid cycle {ECO:0000256|RuleBase:RU000555}.
FT   ACT_SITE    166    166       Proton acceptor. {ECO:0000256|PIRSR:
FT                                PIRSR001363-1}.
FT   ACT_SITE    447    447       Proton donor. {ECO:0000256|PIRSR:
FT                                PIRSR001363-1}.
SQ   SEQUENCE   533 AA;  60377 MW;  6A49C4A2C7AB4398 CRC64;
     MTEQATTIDE LAFTRPYGEQ EKQILTAEAV EFLTELVTHF TPQRNKLLAA RIQQQQDIDN
     GMLPDFISET ASIRDADWKI RGIPSDLEDR RVEITGPVER KMVINALNAN VKVFMADFED
     SLAPDWNKVI DGQINLRDAV NGTISYSNEA GKIYQLKPNP AVLICRVRGL HLPEKHVTWR
     GETIPGSLFD FALYFFHNYQ ALLAKGSGPY FYLPKTQSWQ EAAWWSEVFS YAEDRFNLPR
     GTIKATLLIE TLPAVFQMDE ILHALRDHIV GLNCGRWDYI FSYIKTLKNY PDRVLPDRQA
     VTMDKPFLNA YSRLLIKTCH KRGAFAMGGM AAFIPSKDEE RNNQVLDKVK ADKSLEANNG
     HDGTWIAHPG LADTAMAVFN DILGSRKNQL EVMREQDAPI TADQLLAPCD GERTEEGMRA
     NIRVAVQYIE AWISGNGCVP IYGLMEDAAT AEISRTSIWQ WIHHQKTLSN GKPVTKALFR
     QMLGEEMKVI ASELGEERFS HGRFDDAARL MEQITTSDEL IDFLTLPGYR LLA
//
DBGET integrated database retrieval system