ID E0VQX9_PEDHC Unreviewed; 531 AA.
AC E0VQX9;
DT 02-NOV-2010, integrated into UniProtKB/TrEMBL.
DT 02-NOV-2010, sequence version 1.
DT 27-MAR-2024, entry version 69.
DE RecName: Full=Glucosylceramidase {ECO:0000256|ARBA:ARBA00012658, ECO:0000256|RuleBase:RU361188};
DE EC=3.2.1.45 {ECO:0000256|ARBA:ARBA00012658, ECO:0000256|RuleBase:RU361188};
GN Name=8237528 {ECO:0000313|EnsemblMetazoa:PHUM388700-PA};
GN ORFNames=Phum_PHUM388700 {ECO:0000313|EMBL:EEB15785.1};
OS Pediculus humanus subsp. corporis (Body louse).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Paraneoptera; Psocodea; Phthiraptera; Anoplura; Pediculidae;
OC Pediculus.
OX NCBI_TaxID=121224;
RN [1] {ECO:0000313|EMBL:EEB15785.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=USDA {ECO:0000313|EMBL:EEB15785.1};
RA Kirkness E., Hannick L., Hass B., Bruggner R., Lawson D., Bidwell S.,
RA Joardar V., Caler E., Walenz B., Inman J., Schobel S., Galinsky K.,
RA Amedeo P., Strausberg R.;
RT "Annotation of Pediculus humanus corporis strain USDA.";
RL Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:EEB15785.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=USDA {ECO:0000313|EMBL:EEB15785.1};
RG The Human Body Louse Genome Consortium;
RA Kirkness E., Walenz B., Hass B., Bruggner R., Strausberg R.;
RT "The genome of the human body louse.";
RL Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EnsemblMetazoa:PHUM388700-PA}
RP IDENTIFICATION.
RC STRAIN=USDA {ECO:0000313|EnsemblMetazoa:PHUM388700-PA};
RG EnsemblMetazoa;
RL Submitted (MAY-2020) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-
CC acylsphing-4-enine + D-glucose; Xref=Rhea:RHEA:13269,
CC ChEBI:CHEBI:4167, ChEBI:CHEBI:15377, ChEBI:CHEBI:22801,
CC ChEBI:CHEBI:52639; EC=3.2.1.45;
CC Evidence={ECO:0000256|RuleBase:RU361188};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 30 family.
CC {ECO:0000256|ARBA:ARBA00005382, ECO:0000256|RuleBase:RU361188}.
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DR EMBL; AAZO01004549; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; DS235442; EEB15785.1; -; Genomic_DNA.
DR RefSeq; XP_002428523.1; XM_002428478.1.
DR AlphaFoldDB; E0VQX9; -.
DR STRING; 121224.E0VQX9; -.
DR EnsemblMetazoa; PHUM388700-RA; PHUM388700-PA; PHUM388700.
DR GeneID; 8237528; -.
DR KEGG; phu:Phum_PHUM388700; -.
DR CTD; 8237528; -.
DR VEuPathDB; VectorBase:PHUM388700; -.
DR eggNOG; KOG2566; Eukaryota.
DR HOGENOM; CLU_014379_1_2_1; -.
DR InParanoid; E0VQX9; -.
DR OMA; WIDWNII; -.
DR OrthoDB; 3473901at2759; -.
DR Proteomes; UP000009046; Unassembled WGS sequence.
DR GO; GO:0004348; F:glucosylceramidase activity; IEA:UniProtKB-EC.
DR GO; GO:0006665; P:sphingolipid metabolic process; IEA:UniProtKB-KW.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR033452; GH30_C.
DR InterPro; IPR001139; Glyco_hydro_30.
DR InterPro; IPR033453; Glyco_hydro_30_TIM-barrel.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR11069; GLUCOSYLCERAMIDASE; 1.
DR PANTHER; PTHR11069:SF23; LYSOSOMAL ACID GLUCOSYLCERAMIDASE; 1.
DR Pfam; PF02055; Glyco_hydro_30; 1.
DR Pfam; PF17189; Glyco_hydro_30C; 1.
DR PRINTS; PR00843; GLHYDRLASE30.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|RuleBase:RU361188, ECO:0000313|EMBL:EEB15785.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361188};
KW Lipid metabolism {ECO:0000256|RuleBase:RU361188};
KW Reference proteome {ECO:0000313|Proteomes:UP000009046};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP};
KW Sphingolipid metabolism {ECO:0000256|RuleBase:RU361188}.
FT SIGNAL 1..21
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 22..531
FT /note="Glucosylceramidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5011412605"
FT DOMAIN 103..448
FT /note="Glycosyl hydrolase family 30 TIM-barrel"
FT /evidence="ECO:0000259|Pfam:PF02055"
FT DOMAIN 451..512
FT /note="Glycosyl hydrolase family 30 beta sandwich"
FT /evidence="ECO:0000259|Pfam:PF17189"
SQ SEQUENCE 531 AA; 61247 MW; F1DB52B57CD1323E CRC64;
MTCNSFIIII FLLLFINIFE GRQTDNVECF LRDYGNDNKV CVCNATHCDV INSIPPFPSR
KFNMYVSDKN GLRFSYSRKN FARWMNPKSI KITVNSSETY QKIIGFGGSL SDAAAININS
LSEDARNHFL FSYFSNKGIE YNLLRLPIGG NDYSNRPYTL DDVDNDVSLT YFKLSHEDFN
LKIPNLLKIM NVSDKEVQIV GTAWTAPPWM KTNNDYTGIG TLKEKYYSLW SKYFIRFLEE
YDKFGLKVWG ITTGYQPFTG LIPFYPLNCM GWTPLSQTHW IFQNLGPDLR NSKFNKTNII
ILDDSRFFLP WWPKLVFKNQ KTKAYVNGIG VQWYWNFLFP SRLLDLTQAE FPDKFIISTE
ASFNKWKGTT SPLILGSWER AEEYAESIIE DLNHWVSGWM DSNLALDLKG GPSWAKNFAD
AAIIVNGTGN EFYKQPLFYA MGHFSKFIPK DSIRIDLKIS KSVIKAIGFR TPQKNVVIII
VNKKTYDVPI TILGAKKGNA NFFLPKKAII TVIYRDKKNF FHKCLSKNCK N
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