ID E3G338_ENTCS Unreviewed; 429 AA.
AC E3G338;
DT 11-JAN-2011, integrated into UniProtKB/TrEMBL.
DT 11-JAN-2011, sequence version 1.
DT 29-MAY-2013, entry version 22.
DE RecName: Full=Phosphoribosylamine--glycine ligase;
DE EC=6.3.4.13;
DE AltName: Full=GARS;
DE AltName: Full=Glycinamide ribonucleotide synthetase;
DE AltName: Full=Phosphoribosylglycinamide synthetase;
GN Name=purD; OrderedLocusNames=Entcl_4154;
OS Enterobacter cloacae (strain SCF1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
OX NCBI_TaxID=701347;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SCF1;
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L.,
RA Pitluck S., Davenport K., Detter J.C., Han C., Tapia R., Land M.,
RA Hauser L., Chang Y.-J., Jeffries C., Kyrpides N., Ivanova N.,
RA Mikhailova N., DeAngelis K., Arkin A.P., Chivian D., Edwards B.,
RA Woo H., Hazen T.C., Woyke T.;
RT "Complete sequence of Enterobacter cloacae SCF1.";
RL Submitted (OCT-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY: ATP + 5-phospho-D-ribosylamine + glycine = ADP
CC + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.
CC -!- COFACTOR: Binds 1 magnesium or manganese ion per subunit (By
CC similarity).
CC -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway;
CC N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-
CC ribose 1-diphosphate: step 2/2.
CC -!- SIMILARITY: Belongs to the GARS family.
CC -!- SIMILARITY: Contains 1 ATP-grasp domain.
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DR EMBL; CP002272; ADO50387.1; -; Genomic_DNA.
DR RefSeq; YP_003943671.1; NC_014618.1.
DR EnsemblBacteria; ADO50387; ADO50387; Entcl_4154.
DR GeneID; 9909243; -.
DR KEGG; esc:Entcl_4154; -.
DR PATRIC; 42602582; VBIEntClo171306_4232.
DR HOGENOM; HOG000033463; -.
DR KO; K01945; -.
DR OMA; MGAYTPL; -.
DR BioCyc; ECLO701347:GH9V-4239-MONOMER; -.
DR UniPathway; UPA00074; UER00125.
DR GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR GO; GO:0000287; F:magnesium ion binding; IEA:HAMAP.
DR GO; GO:0030145; F:manganese ion binding; IEA:HAMAP.
DR GO; GO:0004637; F:phosphoribosylamine-glycine ligase activity; IEA:HAMAP.
DR GO; GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0009113; P:purine nucleobase biosynthetic process; IEA:InterPro.
DR Gene3D; 3.30.1490.20; -; 1.
DR Gene3D; 3.30.470.20; -; 1.
DR Gene3D; 3.40.50.20; -; 1.
DR Gene3D; 3.90.600.10; -; 1.
DR HAMAP; MF_00138; GARS; 1; -.
DR InterPro; IPR011761; ATP-grasp.
DR InterPro; IPR013815; ATP_grasp_subdomain_1.
DR InterPro; IPR013816; ATP_grasp_subdomain_2.
DR InterPro; IPR016185; PreATP-grasp_dom.
DR InterPro; IPR020561; PRibGlycinamid_synth_ATP-grasp.
DR InterPro; IPR000115; PRibGlycinamide_synth.
DR InterPro; IPR020560; PRibGlycinamide_synth_C-dom.
DR InterPro; IPR020559; PRibGlycinamide_synth_CS.
DR InterPro; IPR020562; PRibGlycinamide_synth_N.
DR InterPro; IPR011054; Rudment_hybrid_motif.
DR Pfam; PF01071; GARS_A; 1.
DR Pfam; PF02843; GARS_C; 1.
DR Pfam; PF02844; GARS_N; 1.
DR SUPFAM; SSF52440; PreATP-grasp-like; 1.
DR SUPFAM; SSF51246; Rudmnt_hyb_motif; 1.
DR TIGRFAMs; TIGR00877; purD; 1.
DR PROSITE; PS50975; ATP_GRASP; 1.
DR PROSITE; PS00184; GARS; 1.
PE 3: Inferred from homology;
KW ATP-binding; Complete proteome; Ligase; Magnesium; Manganese;
KW Metal-binding; Nucleotide-binding; Purine biosynthesis.
FT DOMAIN 109 316 ATP-grasp (By similarity).
FT NP_BIND 135 196 ATP (By similarity).
FT METAL 286 286 Magnesium or manganese (By similarity).
FT METAL 288 288 Magnesium or manganese (By similarity).
SQ SEQUENCE 429 AA; 45817 MW; D31EDA7993D2F0C8 CRC64;
MKVLIIGNGG REHALAWKAA QSPLVSTVFV APGNAGTALE PALQNVAIGV TDIPELLNFA
QNEKIDLTIV GPEAPLVIGV VDAFRAAGLK IFGPTEGAAQ LEGSKAFTKD FLARHRIPTA
EYQNFTDVEP ALAYLREKGA PIVIKADGLA AGKGVIVAMT LEEAEAAVQD MLAGNAFGDA
GHRIVIEEFL DGEEASFIVM VDGEHVLPMA TSQDHKRVGD GDTGPNTGGM GAYSPAPVVT
DDVHQRTMER IIWPTVRGMA AEGNTYTGFL YAGLMIDKQG NPKVIEFNCR FGDPETQPIM
LRLQSDLVEL CLAACDGKLG DKTSRWDTRA SLGVVMAAGG YPGSYRNGDE IHGLPLEEVE
GGKVFHAGTR LSDDDRVLTN GGRVLCVTAL GNTVAEAQQR AYQLMTDIHW DGSFSRKDIG
YRAIAREQN
//
