GenomeNet

Database: UniProt/TrEMBL
Entry: E3PRA8_ACESD
LinkDB: E3PRA8_ACESD
Original site: E3PRA8_ACESD 
ID   E3PRA8_ACESD            Unreviewed;       483 AA.
AC   E3PRA8;
DT   11-JAN-2011, integrated into UniProtKB/TrEMBL.
DT   11-JAN-2011, sequence version 1.
DT   27-SEP-2017, entry version 35.
DE   SubName: Full=Cytoplasmic alpha-amylase {ECO:0000313|EMBL:CBH20182.1};
DE            EC=3.2.1.1 {ECO:0000313|EMBL:CBH20182.1};
GN   Name=amyA {ECO:0000313|EMBL:CBH20182.1};
GN   OrderedLocusNames=CLOST_0052 {ECO:0000313|EMBL:CBH20182.1};
OS   Acetoanaerobium sticklandii (strain ATCC 12662 / DSM 519 / JCM 1433 /
OS   NCIMB 10654) (Clostridium sticklandii).
OC   Bacteria; Firmicutes; Clostridia; Clostridiales;
OC   Peptostreptococcaceae; Acetoanaerobium.
OX   NCBI_TaxID=499177 {ECO:0000313|EMBL:CBH20182.1, ECO:0000313|Proteomes:UP000007041};
RN   [1] {ECO:0000313|Proteomes:UP000007041}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 12662 / DSM 519 / JCM 1433 / NCIMB 10654
RC   {ECO:0000313|Proteomes:UP000007041};
RX   PubMed=20937090; DOI=10.1186/1471-2164-11-555;
RA   Fonknechten N., Chaussonnerie S., Tricot S., Lajus A., Andreesen J.R.,
RA   Perchat N., Pelletier E., Gouyvenoux M., Barbe V., Salanoubat M.,
RA   Le Paslier D., Weissenbach J., Cohen G.N., Kreimeyer A.;
RT   "Clostridium sticklandii, a specialist in amino acid
RT   degradation:revisiting its metabolism through its genome sequence.";
RL   BMC Genomics 11:555-555(2010).
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; FP565809; CBH20182.1; -; Genomic_DNA.
DR   STRING; 499177.CLOST_0052; -.
DR   EnsemblBacteria; CBH20182; CBH20182; CLOST_0052.
DR   KEGG; cst:CLOST_0052; -.
DR   eggNOG; ENOG4105E5K; Bacteria.
DR   eggNOG; COG0366; LUCA.
DR   HOGENOM; HOG000094847; -.
DR   KO; K01176; -.
DR   OMA; VLNHKMG; -.
DR   OrthoDB; POG091H0HQ3; -.
DR   BioCyc; CSTI499177:GJE9-55-MONOMER; -.
DR   Proteomes; UP000007041; Chromosome.
DR   GO; GO:0004556; F:alpha-amylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0103025; F:alpha-amylase activity (releasing maltohexaose); IEA:UniProtKB-EC.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   InterPro; IPR013776; A-amylase_thermo.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   PIRSF; PIRSF001021; Alph-amls_thrmst; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
PE   4: Predicted;
KW   Calcium {ECO:0000256|PIRSR:PIRSR001021-2};
KW   Complete proteome {ECO:0000313|Proteomes:UP000007041};
KW   Glycosidase {ECO:0000313|EMBL:CBH20182.1};
KW   Hydrolase {ECO:0000313|EMBL:CBH20182.1};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR001021-2};
KW   Reference proteome {ECO:0000313|Proteomes:UP000007041}.
FT   DOMAIN        5    391       Aamy. {ECO:0000259|SMART:SM00642}.
FT   ACT_SITE    233    233       Nucleophile. {ECO:0000256|PIRSR:
FT                                PIRSR001021-1}.
FT   ACT_SITE    263    263       Proton donor. {ECO:0000256|PIRSR:
FT                                PIRSR001021-1}.
FT   METAL       104    104       Calcium 1. {ECO:0000256|PIRSR:
FT                                PIRSR001021-2}.
FT   METAL       196    196       Calcium 1. {ECO:0000256|PIRSR:
FT                                PIRSR001021-2}.
FT   METAL       204    204       Calcium 2. {ECO:0000256|PIRSR:
FT                                PIRSR001021-2}.
FT   METAL       237    237       Calcium 1; via carbonyl oxygen.
FT                                {ECO:0000256|PIRSR:PIRSR001021-2}.
SQ   SEQUENCE   483 AA;  56129 MW;  884049CB1697D2EA CRC64;
     MSLNGIMMQY FEWYLEDDGR LWKKLKDDAN HLKELGITAI WIPPCMKATS TNDTGYGIYD
     LYDLGEFDQK GSIRTKYGTK EELLEAINVL HDNNIKVYAD IVLNHKAAAD ETQRFMAVEV
     DPMDRNKEVS EPYEIEGWTK FNFEGRNNKY SDFKWSWEHF NGTDYNQETE KSAIYLILGE
     NKDWAQDVAE EFGNYDYLMF TNIDYKHPDV YKSTMDWIIW FINETKVDGI RIDAIKHIND
     WFIKDLVLKS RADFGEEFYA VGEYWSVEMP TIESYLSEVD YQLDVFDVPL HFKFHAASYD
     ASNFDMGQLF NDTIVSKYPL NAVTFVDNHD SQPNQSLESH VKQWFKPLAY ASILLRKDGY
     PCIFYGDYYG TRGENKIRPL PDMLDKLIKL RMNHAYGIQE DYLDHKHVIG WVRLGDKENP
     KGCSVIMTST EAGEKTMNVG EMHKGEIWID ALGHTDAEVE IDEKGNGKFI VSDGSVSVYI
     CKD
//
DBGET integrated database retrieval system