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Database: UniProt/TrEMBL
Entry: E4SGZ0_CALK2
LinkDB: E4SGZ0_CALK2
Original site: E4SGZ0_CALK2 
ID   E4SGZ0_CALK2            Unreviewed;       482 AA.
AC   E4SGZ0;
DT   08-FEB-2011, integrated into UniProtKB/TrEMBL.
DT   08-FEB-2011, sequence version 1.
DT   05-JUL-2017, entry version 41.
DE   SubName: Full=Alpha amylase catalytic region {ECO:0000313|EMBL:ADQ47015.1};
GN   OrderedLocusNames=Calkro_2177 {ECO:0000313|EMBL:ADQ47015.1};
OS   Caldicellulosiruptor kronotskyensis (strain DSM 18902 / VKM B-2412 /
OS   2002).
OC   Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales;
OC   Thermoanaerobacterales Family III. Incertae Sedis;
OC   Caldicellulosiruptor.
OX   NCBI_TaxID=632348 {ECO:0000313|EMBL:ADQ47015.1, ECO:0000313|Proteomes:UP000006835};
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=2002;
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L.,
RA   Pitluck S., Davenport K., Detter J.C., Han C., Tapia R., Land M.,
RA   Hauser L., Jeffries C., Kyrpides N., Ivanova N., Mikhailova N.,
RA   Blumer-Schuette S.E., Kelly R.M., Woyke T.;
RT   "Complete sequence of Caldicellulosiruptor kronotskyensis 2002.";
RL   Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:ADQ47015.1, ECO:0000313|Proteomes:UP000006835}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 18902 / VKM B-2412 / 2002
RC   {ECO:0000313|Proteomes:UP000006835};
RX   PubMed=21216991; DOI=10.1128/JB.01515-10;
RA   Blumer-Schuette S.E., Ozdemir I., Mistry D., Lucas S., Lapidus A.,
RA   Cheng J.F., Goodwin L.A., Pitluck S., Land M.L., Hauser L.J.,
RA   Woyke T., Mikhailova N., Pati A., Kyrpides N.C., Ivanova N.,
RA   Detter J.C., Walston-Davenport K., Han S., Adams M.W., Kelly R.M.;
RT   "Complete genome sequences for the anaerobic, extremely thermophilic
RT   plant biomass-degrading bacteria Caldicellulosiruptor hydrothermalis,
RT   Caldicellulosiruptor kristjanssonii, Caldicellulosiruptor
RT   kronotskyensis, Caldicellulosiruptor owensenis, and
RT   Caldicellulosiruptor lactoaceticus.";
RL   J. Bacteriol. 193:1483-1484(2011).
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DR   EMBL; CP002330; ADQ47015.1; -; Genomic_DNA.
DR   RefSeq; WP_013431087.1; NC_014720.1.
DR   CAZy; GH13; Glycoside Hydrolase Family 13.
DR   EnsemblBacteria; ADQ47015; ADQ47015; Calkro_2177.
DR   KEGG; ckn:Calkro_2177; -.
DR   PATRIC; fig|632348.3.peg.2292; -.
DR   HOGENOM; HOG000031038; -.
DR   KO; K01176; -.
DR   OMA; RLNYNQD; -.
DR   OrthoDB; POG091H0HQ3; -.
DR   Proteomes; UP000006835; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   InterPro; IPR013776; A-amylase_thermo.
DR   InterPro; IPR015237; Alpha-amylase_C_pro.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   Pfam; PF09154; DUF1939; 1.
DR   PIRSF; PIRSF001021; Alph-amls_thrmst; 2.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
PE   4: Predicted;
KW   Calcium {ECO:0000256|PIRSR:PIRSR001021-2};
KW   Complete proteome {ECO:0000313|Proteomes:UP000006835};
KW   Membrane {ECO:0000256|SAM:Phobius};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR001021-2};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM     12     35       Helical. {ECO:0000256|SAM:Phobius}.
FT   DOMAIN       34    375       Aamy. {ECO:0000259|SMART:SM00642}.
FT   ACT_SITE    226    226       Nucleophile. {ECO:0000256|PIRSR:
FT                                PIRSR001021-1}.
FT   ACT_SITE    251    251       Proton donor. {ECO:0000256|PIRSR:
FT                                PIRSR001021-1}.
FT   METAL       139    139       Calcium 1. {ECO:0000256|PIRSR:
FT                                PIRSR001021-2}.
FT   METAL       193    193       Calcium 1. {ECO:0000256|PIRSR:
FT                                PIRSR001021-2}.
FT   METAL       289    289       Calcium 3; via carbonyl oxygen.
FT                                {ECO:0000256|PIRSR:PIRSR001021-2}.
FT   METAL       394    394       Calcium 3. {ECO:0000256|PIRSR:
FT                                PIRSR001021-2}.
FT   METAL       416    416       Calcium 3. {ECO:0000256|PIRSR:
FT                                PIRSR001021-2}.
SQ   SEQUENCE   482 AA;  53810 MW;  07DFDD19C5392E72 CRC64;
     MNGLFKKLSK KLTIISLIVI IVFLLTSSLS IYAGVMMQGF YWDVPAGGTW WNTLASKAYE
     LKYMVGGSYG INRIWFPPAY KGQGGAYSMG YDPHDYYDLG QYYQDGTTET RFGSQSELKN
     AISKYKSYGI SVTEDIVLNH RSGGKSEYNP KTGTNTWTDF TNTASGMCQW HWDAFHPNNY
     CSGDEGTFAG FPDVCYTSGP AYNDMKAWMN WLKSSTNAGF DSWRYDYVKG YGYWVVKDFN
     AATSPTFSVG EYWDANTSTL DWWANSSGSS VFDFALYYTL RDICNNTSGS GYLPNVFDYS
     KSYAAKNPFK AVTFVANHDT DEIVNDKMMA YAFILTYQGY PCIFWKDYYD YGLATGGGAS
     PGGWGNGIKQ LVWCREKLAA GAPNIEILKS NDGDIIIYGS KGYSTSSPGY IVVINDHPSQ
     WKGAWVQTSN SYLKGKTLKA YAWSSTVSGQ NVQPQNKYCD ANGWVEVWAP PRGYAVYSVD
     GL
//
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