ID E4TEJ4_CALNY Unreviewed; 479 AA.
AC E4TEJ4;
DT 08-FEB-2011, integrated into UniProtKB/TrEMBL.
DT 08-FEB-2011, sequence version 1.
DT 01-MAY-2013, entry version 19.
DE RecName: Full=Cysteine--tRNA ligase;
DE EC=6.1.1.16;
DE AltName: Full=Cysteinyl-tRNA synthetase;
GN Name=cysS; OrderedLocusNames=Calni_0407;
OS Calditerrivibrio nitroreducens (strain DSM 19672 / NBRC 101217 /
OS Yu37-1).
OC Bacteria; Deferribacteres; Deferribacterales; Deferribacteraceae.
OX NCBI_TaxID=768670;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 19672;
RG US DOE Joint Genome Institute (JGI-PGF);
RA Lucas S., Copeland A., Lapidus A., Bruce D., Goodwin L., Pitluck S.,
RA Kyrpides N., Mavromatis K., Ivanova N., Mikhailova N., Zeytun A.,
RA Brettin T., Detter J.C., Tapia R., Han C., Land M., Hauser L.,
RA Markowitz V., Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Spring S.,
RA Schroeder M., Brambilla E., Klenk H.-P., Eisen J.A.;
RT "The complete genome of chromosome of Calditerrivibrio nitroreducens
RT DSM 19672.";
RL Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 19672 / NBRC 101217 / Yu37-1;
RX PubMed=21475587; DOI=10.4056/sigs.1523807;
RA Pitluck S., Sikorski J., Zeytun A., Lapidus A., Nolan M., Lucas S.,
RA Hammon N., Deshpande S., Cheng J.F., Tapia R., Han C., Goodwin L.,
RA Liolios K., Pagani I., Ivanova N., Mavromatis K., Pati A., Chen A.,
RA Palaniappan K., Hauser L., Chang Y.J., Jeffries C.D., Detter J.C.,
RA Brambilla E., Djao O.D., Rohde M., Spring S., Goker M., Woyke T.,
RA Bristow J., Eisen J.A., Markowitz V., Hugenholtz P., Kyrpides N.C.,
RA Klenk H.P., Land M.;
RT "Complete genome sequence of Calditerrivibrio nitroreducens type
RT strain (Yu37-1).";
RL Stand. Genomic Sci. 4:54-62(2011).
CC -!- CATALYTIC ACTIVITY: ATP + L-cysteine + tRNA(Cys) = AMP +
CC diphosphate + L-cysteinyl-tRNA(Cys).
CC -!- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
CC -!- SUBUNIT: Monomer (By similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase
CC family.
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DR EMBL; CP002347; ADR18320.1; -; Genomic_DNA.
DR RefSeq; YP_004050483.1; NC_014758.1.
DR ProteinModelPortal; E4TEJ4; -.
DR EnsemblBacteria; ADR18320; ADR18320; Calni_0407.
DR GeneID; 10008414; -.
DR KEGG; cni:Calni_0407; -.
DR PATRIC; 45188508; VBICalNit163602_0473.
DR HOGENOM; HOG000245250; -.
DR KO; K01883; -.
DR OMA; DFDALNM; -.
DR BioCyc; CNIT768670:GHD1-416-MONOMER; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR GO; GO:0004817; F:cysteine-tRNA ligase activity; IEA:HAMAP.
DR GO; GO:0008270; F:zinc ion binding; IEA:HAMAP.
DR GO; GO:0006423; P:cysteinyl-tRNA aminoacylation; IEA:HAMAP.
DR Gene3D; 3.40.50.620; -; 1.
DR HAMAP; MF_00041; Cys_tRNA_synth; 1; -.
DR InterPro; IPR015803; Cys-tRNA-ligase.
DR InterPro; IPR015273; Cys-tRNA-synt_Ia_DALR.
DR InterPro; IPR024909; Cys-tRNA/MSH_ligase.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_1a_anticodon-bd.
DR PANTHER; PTHR10890; PTHR10890; 1.
DR Pfam; PF09190; DALR_2; 1.
DR Pfam; PF01406; tRNA-synt_1e; 1.
DR PRINTS; PR00983; TRNASYNTHCYS.
DR SMART; SM00840; DALR_2; 1.
DR SUPFAM; SSF47323; tRNAsyn_1a_bind; 1.
DR TIGRFAMs; TIGR00435; cysS; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm;
KW Ligase; Metal-binding; Nucleotide-binding; Protein biosynthesis; Zinc.
FT MOTIF 30 40 "HIGH" region (By similarity).
FT MOTIF 265 269 "KMSKS" region (By similarity).
FT METAL 28 28 Zinc (By similarity).
FT METAL 208 208 Zinc (By similarity).
FT METAL 233 233 Zinc (By similarity).
FT METAL 237 237 Zinc (By similarity).
FT BINDING 268 268 ATP (By similarity).
SQ SEQUENCE 479 AA; 55363 MW; 1984E8F34C796789 CRC64;
MLKIFNTLSG EKEVFTPLED NKVKIYVCGV TVYDYCHIGH ARSSIVFDTI RRYLKYKGFD
VTFVKNFTDV DDKIIKRANE ENKSINEITD FFIKAHDEDM DKLNILRPDY TPRATEYIDG
MIKLCEKLIE KGFAYESNGD VYFKVRAFKD YGKLSKRDLD DLLAGARVDV NEIKEDPLDF
ALWKRSKEGE PGWDSPWGRG RPGWHIECSV MSAEILGIPF DIHGGGKDLI FPHHENEIAQ
SEAAEGKEFA RYWIHNGFVN INKEKMSKSL GNFFTIRDVL KEVDPEVLRF FLLTTHYRSP
LDFSFENLLE AENALDRIYT AIDELESAVF NNKKPAQKGK IDEIVDRFKR DFEESMDDDF
NTASAISNIF ELIKGVNLLL SERLNSEDLN YLKVRFEEVK AIIRDVLGIL TKTASEWFKM
NLSIDESELQ KLIEEREIAR RNKDFSKADA IRNELKTKGV ELLDTPTGTK FRAKRLRGG
//
