ID E4UB39_LIBSC Unreviewed; 231 AA.
AC E4UB39;
DT 08-FEB-2011, integrated into UniProtKB/TrEMBL.
DT 08-FEB-2011, sequence version 1.
DT 01-MAY-2013, entry version 19.
DE RecName: Full=Orotate phosphoribosyltransferase;
DE Short=OPRT;
DE Short=OPRTase;
DE EC=2.4.2.10;
GN Name=pyrE; OrderedLocusNames=CKC_03845;
OS Liberibacter solanacearum (strain CLso-ZC1).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC Rhizobiaceae; Candidatus Liberibacter.
OX NCBI_TaxID=658172;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CLso-ZC1;
RA Lin H., Doddapaneni H.V., Lou B., Civerolo E.L., Chen C., Duan Y.,
RA Zhou L., Glynn J.;
RT "Complete genome sequence of Candidatus Liberibacter solanacearum
RT CLso-ZC1.";
RL Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=CLso-ZC1;
RA Lin H., Doddapaneni H.V., Lou B., Civerolo E.L., Chen C., Duan Y.,
RA Zhou L., Glynn J.;
RL Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the transfer of a ribosyl phosphate group from
CC 5-phosphoribose 1-diphosphate to orotate, leading to the formation
CC of orotidine monophosphate (OMP) (By similarity).
CC -!- CATALYTIC ACTIVITY: Orotidine 5'-phosphate + diphosphate = orotate
CC + 5-phospho-alpha-D-ribose 1-diphosphate.
CC -!- COFACTOR: Magnesium (By similarity).
CC -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo
CC pathway; UMP from orotate: step 1/2.
CC -!- SUBUNIT: Homodimer (By similarity).
CC -!- SIMILARITY: Belongs to the purine/pyrimidine
CC phosphoribosyltransferase family. PyrE subfamily.
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DR EMBL; CP002371; ADR52518.1; -; Genomic_DNA.
DR RefSeq; YP_004063006.1; NC_014774.1.
DR EnsemblBacteria; ADR52518; ADR52518; CKC_03845.
DR GeneID; 10034142; -.
DR KEGG; lso:CKC_03845; -.
DR PATRIC; 45199003; VBICanLib97138_0768.
DR HOGENOM; HOG000037975; -.
DR KO; K00762; -.
DR BioCyc; LSOL658172:GHHM-798-MONOMER; -.
DR UniPathway; UPA00070; UER00119.
DR GO; GO:0000287; F:magnesium ion binding; IEA:HAMAP.
DR GO; GO:0004588; F:orotate phosphoribosyltransferase activity; IEA:HAMAP.
DR GO; GO:0044205; P:'de novo' UMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR HAMAP; MF_01208; PyrE; 1; -.
DR InterPro; IPR023031; OPRT.
DR InterPro; IPR000836; PRibTrfase_dom.
DR Pfam; PF00156; Pribosyltran; 1.
PE 3: Inferred from homology;
KW Complete proteome; Glycosyltransferase; Magnesium;
KW Pyrimidine biosynthesis; Transferase.
FT REGION 133 141 5-phosphoribose 1-diphosphate binding (By
FT similarity).
FT BINDING 107 107 5-phosphoribose 1-diphosphate; shared
FT with dimeric partner (By similarity).
FT BINDING 108 108 5-phosphoribose 1-diphosphate (By
FT similarity).
FT BINDING 111 111 5-phosphoribose 1-diphosphate; shared
FT with dimeric partner (By similarity).
FT BINDING 113 113 5-phosphoribose 1-diphosphate; shared
FT with dimeric partner (By similarity).
FT BINDING 137 137 Orotate (By similarity).
SQ SEQUENCE 231 AA; 26173 MW; 4EF4F3073C3224D7 CRC64;
MIFSDFPEPK FMAELVAKML FEIKAVNFSP EKPYRLTSGI VSPVYIDCRK LISFARVRST
IMNFATTIVL RNIGFESIDI IAGGETAGIP FAAFLAERLS LPMIYVRKKT KNHGRKSQIE
GHIFKGARIL IIEDLITLGG SMVDFVQVIR KSGGVVKNGI GLFFYDIFPD ITVRLRENDI
NLHYLATWND IMKVATNLKI FNQDILDEVQ YFLDNPMIWS EKNGGIGKIE K
//
