ID   E6SI00_THEM7            Unreviewed;       631 AA.
AC   E6SI00;
DT   08-MAR-2011, integrated into UniProtKB/TrEMBL.
DT   08-MAR-2011, sequence version 1.
DT   29-MAY-2013, entry version 21.
DE   RecName: Full=Acetolactate synthase;
DE            EC=2.2.1.6;
DE   Flags: Precursor;
GN   OrderedLocusNames=Tmar_1778;
OS   Thermaerobacter marianensis (strain ATCC 700841 / DSM 12885 / JCM
OS   10246 / 7p75a).
OC   Bacteria; Firmicutes; Clostridia; Clostridiales;
OC   Clostridiales Family XVII. Incertae Sedis; Thermaerobacter.
OX   NCBI_TaxID=644966;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700841 / DSM 12885 / JCM 10246 / 7p75a;
RX   DOI=10.4056/sigs.1373474;
RA   Han C., Gu W., Zhang X., Lapidus A., Nolan M., Copeland A., Lucas S.,
RA   Glavina Del Rio T., Tice H., Cheng J., Tapia R., Goodwin L.,
RA   Pitluck S., Pagani I., Ivanova N., Mavromatis K., Mikhailova N.,
RA   Pati A., Chen A., Palaniappan K., Land M., Hauser L., Chang Y.,
RA   Jeffries C., Schneider S., Rohde M., Goker M., Pukall R., Woyke T.,
RA   Bristow J., Eisen J., Markowitz V., Hugenholtz P., Kyrpides N.,
RA   Klenk H., Detter J.;
RT   "Complete genome sequence of Thermaerobacter marianensis type strain
RT   (7p75aT).";
RL   Stand. Genomic Sci. 3:337-345(2010).
CC   -!- CATALYTIC ACTIVITY: 2 pyruvate = 2-acetolactate + CO(2).
CC   -!- COFACTOR: Binds 1 magnesium ion per subunit (By similarity).
CC   -!- COFACTOR: Binds 1 thiamine pyrophosphate per subunit (By
CC       similarity).
CC   -!- PATHWAY: Amino-acid biosynthesis; L-isoleucine biosynthesis; L-
CC       isoleucine from 2-oxobutanoate: step 1/4.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-valine biosynthesis; L-valine
CC       from pyruvate: step 1/4.
CC   -!- SIMILARITY: Belongs to the TPP enzyme family.
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DR   EMBL; CP002344; ADU51880.1; -; Genomic_DNA.
DR   RefSeq; YP_004102607.1; NC_014831.1.
DR   EnsemblBacteria; ADU51880; ADU51880; Tmar_1778.
DR   GeneID; 10082161; -.
DR   KEGG; tmr:Tmar_1778; -.
DR   PATRIC; 45380838; VBITheMar50867_1825.
DR   HOGENOM; HOG000258448; -.
DR   KO; K01652; -.
DR   OMA; MENIAYK; -.
DR   BioCyc; TMAR644966:GHKT-1817-MONOMER; -.
DR   UniPathway; UPA00047; UER00055.
DR   UniPathway; UPA00049; UER00059.
DR   GO; GO:0003984; F:acetolactate synthase activity; IEA:EC.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0030976; F:thiamine pyrophosphate binding; IEA:InterPro.
DR   GO; GO:0009097; P:isoleucine biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0009099; P:valine biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR012846; Acetolactate_synth_lsu.
DR   InterPro; IPR012000; Thiamin_PyroP_enz_cen_dom.
DR   InterPro; IPR012001; Thiamin_PyroP_enz_TPP-bd_dom.
DR   InterPro; IPR000399; TPP-bd_CS.
DR   InterPro; IPR011766; TPP_enzyme-bd_C.
DR   PANTHER; PTHR18968:SF13; PTHR18968:SF13; 1.
DR   Pfam; PF02775; TPP_enzyme_C; 1.
DR   Pfam; PF00205; TPP_enzyme_M; 1.
DR   Pfam; PF02776; TPP_enzyme_N; 1.
DR   TIGRFAMs; TIGR00118; acolac_lg; 1.
DR   PROSITE; PS00187; TPP_ENZYMES; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Branched-chain amino acid biosynthesis;
KW   Complete proteome; Magnesium; Metal-binding; Signal;
KW   Thiamine pyrophosphate; Transferase.
FT   SIGNAL        1     33       Potential.
FT   CHAIN        34    631       Potential.
FT                                /FTId=PRO_5000669299.
SQ   SEQUENCE   631 AA;  66973 MW;  2C93F011D16303F3 CRC64;
     MSQPAKQATA TATVTAAGGP PPAARPPSTP PHAMTGAGLR PGTETPPVNR RGPMSGADVV
     VQVLLEQGVE WVFGIPGGAV LPLYDALYGA PIRHVLARHE QGAALAADGY ARASGRVGVC
     IATSGPGATN LITGLATSYL DSVPVVAITG NVGRAFLGTD AFQEADTFGV SMPVTKHNYL
     VLDPEELPAI LREAFAVAAS GRPGPVLVDI PKDIFTATIS TGAWDRPLRR SVSCFGRTAA
     DSGTPHIPDL PGRSHLTGRL EEAARLIARA RQPVLYAGGG VIVAGAAEAL RRLAEQAVLP
     TTTTLMALGA MPGDHPLFLG MPGMHGTYAA NMALTETDCL VAVGARFDDR VTGKVSAFAP
     DAAIIHIDVD AAELGKVKAP RVAIPADARE GLEALREALG RVPPDEWADR SPWLERVNGW
     KRDHPYRYDR ERARRELLPQ AVIEELERAT GGDALVVTGV GQHQMWAAMF YRYRRPRQFL
     TSGGLGTMGY GLPAAIGAQL ARPDARVLCI DGDGSFQMNV QELSTLAELG LPVKVFIINN
     RAHGMVRQWQ DLFYGQRLSA SVFEHQPDFV KLAEAYGIAA FRITHPDELA GTVREALARP
     GPVVVDCVVR QEENVLPMVP PGAALREMIV G
//