ID E8RA45_DESM0 Unreviewed; 629 AA.
AC E8RA45;
DT 05-APR-2011, integrated into UniProtKB/TrEMBL.
DT 05-APR-2011, sequence version 1.
DT 01-MAY-2013, entry version 16.
DE RecName: Full=Phosphoenolpyruvate carboxykinase [GTP];
DE Short=PEP carboxykinase;
DE Short=PEPCK;
DE EC=4.1.1.32;
DE AltName: Full=Phosphoenolpyruvate carboxylase;
GN Name=pckG; OrderedLocusNames=Desmu_0004;
OS Desulfurococcus mucosus (strain ATCC 35584 / DSM 2162 / JCM 9187 /
OS O7/1).
OC Archaea; Crenarchaeota; Thermoprotei; Desulfurococcales;
OC Desulfurococcaceae; Desulfurococcus.
OX NCBI_TaxID=765177;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35584 / DSM 2162 / JCM 9187 / O7/1;
RG US DOE Joint Genome Institute (JGI-PGF);
RA Lucas S., Copeland A., Lapidus A., Bruce D., Goodwin L., Pitluck S.,
RA Kyrpides N., Mavromatis K., Pagani I., Ivanova N., Ovchinnikova G.,
RA Chertkov O., Held B., Brettin T., Detter J.C., Tapia R., Han C.,
RA Land M., Hauser L., Markowitz V., Cheng J.-F., Hugenholtz P.,
RA Woyke T., Wu D., Wirth R., Bilek Y., Hader T., Klenk H.-P.,
RA Eisen J.A.;
RT "The complete genome of Desulfurococcus mucosus DSM 2162.";
RL Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the conversion of oxaloacetate (OAA) to
CC phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic
CC pathway that produces glucose from lactate and other precursors
CC derived from the citric acid cycle (By similarity).
CC -!- CATALYTIC ACTIVITY: GTP + oxaloacetate = GDP + phosphoenolpyruvate
CC + CO(2).
CC -!- COFACTOR: Binds 1 manganese ion per subunit (By similarity).
CC -!- PATHWAY: Carbohydrate biosynthesis; gluconeogenesis.
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- SIMILARITY: Belongs to the phosphoenolpyruvate carboxykinase [GTP]
CC family.
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DR EMBL; CP002363; ADV64323.1; -; Genomic_DNA.
DR RefSeq; YP_004175805.1; NC_014961.1.
DR EnsemblBacteria; ADV64323; ADV64323; Desmu_0004.
DR GeneID; 10152681; -.
DR KEGG; dmu:Desmu_0004; -.
DR HOGENOM; HOG000191700; -.
DR KO; K01596; -.
DR OMA; WMRFGED; -.
DR BioCyc; DMUC765177:GHQE-4-MONOMER; -.
DR UniPathway; UPA00138; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:HAMAP.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0030145; F:manganese ion binding; IEA:HAMAP.
DR GO; GO:0004613; F:phosphoenolpyruvate carboxykinase (GTP) activity; IEA:HAMAP.
DR GO; GO:0006094; P:gluconeogenesis; IEA:HAMAP.
DR Gene3D; 3.40.449.10; -; 1.
DR Gene3D; 3.90.228.20; -; 2.
DR HAMAP; MF_00452; PEPCK_GTP; 1; -.
DR InterPro; IPR013035; PEP_carboxykinase_C.
DR InterPro; IPR008209; PEP_carboxykinase_GTP.
DR InterPro; IPR008210; PEP_carboxykinase_N.
DR PANTHER; PTHR11561; PTHR11561; 1.
DR Pfam; PF00821; PEPCK; 1.
DR PIRSF; PIRSF001348; PEP_carboxykinase_GTP; 1.
DR SUPFAM; SSF68923; PEP_carboxykinase_N; 1.
PE 3: Inferred from homology;
KW Complete proteome; Cytoplasm; Decarboxylase; Gluconeogenesis;
KW GTP-binding; Kinase; Lyase; Manganese; Metal-binding;
KW Nucleotide-binding; Pyruvate.
FT REGION 390 392 Substrate binding (By similarity).
FT ACT_SITE 278 278 By similarity.
FT METAL 235 235 Manganese (By similarity).
FT METAL 254 254 Manganese (By similarity).
FT METAL 294 294 Manganese (By similarity).
FT BINDING 92 92 Substrate (By similarity).
FT BINDING 228 228 Substrate; via amide nitrogen (By
FT similarity).
FT BINDING 235 235 Substrate (By similarity).
FT BINDING 392 392 GTP (By similarity).
FT BINDING 423 423 GTP (By similarity).
SQ SEQUENCE 629 AA; 71760 MW; A6F7264A09719A7B CRC64;
MDASVTGLLT RLSRYADERA VERLSRISDR RLLEWITEVV ELLEPASVFV NTGSREDLEY
IRMRAVEKRE EIPSRYNPRH TVHFDGIYDL ARDRENTRIL TPGGSPIPMV NTGNRDEGLR
ELREIYRGIM RGKEMYIGFY CFGPRGSPFT LYGVQVTDSA YVMHSENILY RVCYDVFVEK
GGSMRYMRFL HSAGELNEYG WSRNISKRRI YIDTVDNITY SVNTQYAGNT VGLKKLSLRL
CVNQGYRENW LCEHMFIVGV KGTGDTISYF TGAFPAGCGK TSTALIADTV VGDDLAMIRN
VNGEARAVNP EIGMFGIIDG VNPVDDPEIY GILSSRETEV IFSNVLLTED GEVWWNGKPS
EPRRGVNYAG EWWPGKLDEK GRPIPPSHPN ARFTTSIFYL SKVDPRINDP NGVPVSGMIF
GGRDSDTWVP VEEAFNWVHG IVTKGASLES ERTTAVLGKA GEREFNPFAI LDFLSVSPGE
FIELHFRFGD GLEKQPRIYG VNYFLRDEAG RYLTEKVDKR VWLKWMALRV NNEVDALETP
TGLIPVYEDL AVLFKRELGK EFSIELYEKL FTVRVGKHLE KAERILRIYE NIPGTPRLFF
DTLIEQKRRL KHALDKYGMF ISPLSLDKR
//
