ID F4GYZ1_CELFA Unreviewed; 701 AA.
AC F4GYZ1;
DT 28-JUN-2011, integrated into UniProtKB/TrEMBL.
DT 28-JUN-2011, sequence version 1.
DT 03-APR-2013, entry version 16.
DE RecName: Full=UvrABC system protein B;
DE Short=Protein UvrB;
DE AltName: Full=Excinuclease ABC subunit B;
GN Name=uvrB; OrderedLocusNames=Celf_1851;
OS Cellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513
OS / NCIMB 8980 / NCTC 7547).
OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC Micrococcineae; Cellulomonadaceae; Cellulomonas.
OX NCBI_TaxID=590998;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 /
RC NCTC 7547;
RG US DOE Joint Genome Institute;
RA Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S.,
RA Peters L., Chertkov O., Detter J.C., Han C., Tapia R., Land M.,
RA Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Pagani I.,
RA Mead D., Brumm P., Woyke T.;
RT "Complete sequence of Cellulomonas fimi ATCC 484.";
RL Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: The UvrABC repair system catalyzes the recognition and
CC processing of DNA lesions. A damage recognition complex composed
CC of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon
CC binding of the UvrA(2)B(2) complex to a putative damaged site, the
CC DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP
CC binding by UvrB and probably causes local melting of the DNA
CC helix, facilitating insertion of UvrB beta-hairpin between the DNA
CC strands. Then UvrB probes one DNA strand for the presence of a
CC lesion. If a lesion is found the UvrA subunits dissociate and the
CC UvrB-DNA preincision complex is formed. This complex is
CC subsequently bound by UvrC and the second UvrB is released. If no
CC lesion is found, the DNA wraps around the other UvrB subunit that
CC will check the other stand for damage (By similarity).
CC -!- SUBUNIT: Forms a heterotetramer with UvrA during the search for
CC lesions. Interacts with UvrC in an incision complex (By
CC similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- DOMAIN: The beta-hairpin motif is involved in DNA binding (By
CC similarity).
CC -!- SIMILARITY: Belongs to the UvrB family.
CC -!- SIMILARITY: Contains 1 UVR domain.
CC -!- SIMILARITY: Contains 1 helicase ATP-binding domain.
CC -!- SIMILARITY: Contains 1 helicase C-terminal domain.
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DR EMBL; CP002666; AEE45981.1; -; Genomic_DNA.
DR RefSeq; YP_004453368.1; NC_015514.1.
DR EnsemblBacteria; AEE45981; AEE45981; Celf_1851.
DR GeneID; 10592633; -.
DR KEGG; cfi:Celf_1851; -.
DR PATRIC; 54614630; VBICelFim18861_1853.
DR KO; K03702; -.
DR BioCyc; CFIM590998:GJFK-1873-MONOMER; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR GO; GO:0003677; F:DNA binding; IEA:HAMAP.
DR GO; GO:0009381; F:excinuclease ABC activity; IEA:HAMAP.
DR GO; GO:0004386; F:helicase activity; IEA:HAMAP.
DR GO; GO:0090305; P:nucleic acid phosphodiester bond hydrolysis; IEA:GOC.
DR GO; GO:0006289; P:nucleotide-excision repair; IEA:HAMAP.
DR GO; GO:0009432; P:SOS response; IEA:HAMAP.
DR Gene3D; 4.10.860.10; -; 1.
DR HAMAP; MF_00204; UvrB; 1; -.
DR InterPro; IPR006935; Helicase/UvrB_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR001943; UVR_dom.
DR InterPro; IPR004807; UvrB.
DR InterPro; IPR024759; UvrB_YAD/RRR_dom.
DR PANTHER; PTHR24029; PTHR24029; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF04851; ResIII; 1.
DR Pfam; PF02151; UVR; 1.
DR Pfam; PF12344; UvrB; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF46600; UvrB_C; 1.
DR TIGRFAMs; TIGR00631; uvrb; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS50151; UVR; 1.
PE 3: Inferred from homology;
KW ATP-binding; Complete proteome; Cytoplasm; DNA damage; DNA excision;
KW DNA repair; Excision nuclease; Helicase; Hydrolase;
KW Nucleotide-binding; SOS response.
FT DOMAIN 35 192 Helicase ATP-binding (By similarity).
FT DOMAIN 438 604 Helicase C-terminal (By similarity).
FT DOMAIN 657 692 UVR (By similarity).
FT NP_BIND 48 55 ATP (By similarity).
FT MOTIF 101 124 Beta-hairpin (By similarity).
SQ SEQUENCE 701 AA; 78433 MW; F11FEE83DAC60F48 CRC64;
MRPVTELQRT VAPFQVVSEF TPSGDQPAAI AELASRINAG EKDVVLLGAT GTGKSATTAW
LIEQVQRPTL VMAPNKTLAA QLATEFRELL PNNAVEYFVS YYDYYQPEAY IVQTDTYIEK
DSSINDEVER LRHSATSSLL TRRDVVVVAS VSCIYGLGTP QEYVDRMVTL DVGQQIDRDQ
LLRRFVQMQY TRNDLAFTRG TFRVRGDTVE IIPMYEELAI RIEFFGDEIE AIATLHPLTG
DVVRSEDQMM IFPATHYVAG PERMERAIAG IELELEDRLA ELESQNKLLE AQRLRMRTTY
DIEMMRQIGS CSGIENYSRH IDGRTAGSAP NTLLDFFPED FLLVIDESHV TVPQIGAMFE
GDMSRKRALV EHGFRLPSAT DNRPLRWEEF VERIGQTVYL SATPGDYELS MADGVVEQII
RPTGLVDPEV VVKPTKGQID DLLGEIHDRV ERDERVLVTT LTKKMAEDLT DYFLEKGVRV
RYLHSEVDTL RRVELLRELR LGEYDVLVGI NLLREGLDLP EVSLVAILDA DKEGFLRSGK
SLIQTIGRAA RNVSGQVHMY ADKITPAMQL AIEETSRRRE KQIAYNTERG IDPQPLRKKI
GDITDLLARE DADTAELLGG AGRQASRGKA PVPGFGSKGA PRDERTRLTG AAAGELAGLI
QELTDQMHAA AGELQFELAA RLRDEISGLK KELRQMQAAT A
//
