ID F6EQR2_AMYSD Unreviewed; 322 AA.
AC F6EQR2;
DT 27-JUL-2011, integrated into UniProtKB/TrEMBL.
DT 27-JUL-2011, sequence version 1.
DT 03-APR-2013, entry version 12.
DE RecName: Full=4-diphosphocytidyl-2-C-methyl-D-erythritol kinase;
DE Short=CMK;
DE EC=2.7.1.148;
DE AltName: Full=4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase;
GN Name=ispE; OrderedLocusNames=AS9A_3499;
OS Amycolicicoccus subflavus (strain DSM 45089 / DQS3-9A1).
OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC Corynebacterineae; Mycobacteriaceae; Amycolicicoccus.
OX NCBI_TaxID=443218;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 45089 / DQS3-9A1;
RX PubMed=21725023; DOI=10.1128/JB.05388-11;
RA Cai M., Chen W.M., Nie Y., Chi C.Q., Wang Y.N., Tang Y.Q., Li G.Y.,
RA Wu X.L.;
RT "Complete genome sequence of Amycolicicoccus subflavus DQS3-9A1T, an
RT actinomycete isolated from crude oil-polluted soil.";
RL J. Bacteriol. 193:4538-4539(2011).
CC -!- FUNCTION: Catalyzes the phosphorylation of the position 2 hydroxy
CC group of 4-diphosphocytidyl-2C-methyl-D-erythritol (By
CC similarity).
CC -!- CATALYTIC ACTIVITY: ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-
CC erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-
CC D-erythritol.
CC -!- PATHWAY: Isoprenoid biosynthesis; isopentenyl diphosphate
CC biosynthesis via DXP pathway; isopentenyl diphosphate from 1-
CC deoxy-D-xylulose 5-phosphate: step 3/6.
CC -!- SIMILARITY: Belongs to the GHMP kinase family. IspE subfamily.
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DR EMBL; CP002786; AEF41939.1; -; Genomic_DNA.
DR RefSeq; YP_004494739.1; NC_015564.1.
DR EnsemblBacteria; AEF41939; AEF41939; AS9A_3499.
DR GeneID; 10635667; -.
DR KEGG; asd:AS9A_3499; -.
DR KO; K00919; -.
DR BioCyc; ASUB443218:GH9R-3535-MONOMER; -.
DR UniPathway; UPA00056; UER00094.
DR GO; GO:0050515; F:4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity; IEA:HAMAP.
DR GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR GO; GO:0019288; P:isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway; IEA:UniProtKB-UniPathway.
DR GO; GO:0016114; P:terpenoid biosynthetic process; IEA:HAMAP.
DR Gene3D; 3.30.230.10; -; 1.
DR HAMAP; MF_00061; IspE; 1; -.
DR InterPro; IPR013750; GHMP_kinase_C_dom.
DR InterPro; IPR006204; GHMP_kinase_N_dom.
DR InterPro; IPR004424; IspE.
DR InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR InterPro; IPR014721; Ribosomal_S5_D2-typ_fold_subgr.
DR PANTHER; PTHR20861:SF2; PTHR20861:SF2; 1.
DR Pfam; PF08544; GHMP_kinases_C; 1.
DR Pfam; PF00288; GHMP_kinases_N; 1.
DR PIRSF; PIRSF010376; IspE; 1.
DR SUPFAM; SSF54211; Ribosomal_S5_D2-typ_fold; 1.
DR TIGRFAMs; TIGR00154; ispE; 1.
PE 3: Inferred from homology;
KW ATP-binding; Complete proteome; Isoprene biosynthesis; Kinase;
KW Nucleotide-binding; Transferase.
FT NP_BIND 101 111 ATP (By similarity).
FT ACT_SITE 16 16 By similarity.
FT ACT_SITE 143 143 By similarity.
SQ SEQUENCE 322 AA; 33465 MW; 191821039B31520E CRC64;
MLHVVDEPVK VRAPAKVNLH LAVGDLRDDG YHELTTVFQA LSLYDEVAVT RGDALTVSVK
GEGAREVPLD ENNLVWRAAQ LMAEKAGRSP RIHIAIRKGI PVAGGLAGGS ADAAATLVAL
NTLWHLELSR GELSDLAAEL GSDVPFSLRG GTAMGIGRGE QLLPVLSRST YHWVLAFANG
GLSTPAVYKE LDRLRESGTR PRLGSADDLM HALTVGDPDA LAPLLGNDLQ AAAISLKPQL
RRTLRAGKDA GALAAIVSGS GPTCAFLCRD AEDAVSVGVE VSATGVCRTV RTATGPVPGA
RVIDTDSGPD DPAQERAVDG GA
//
