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Database: UniProt/TrEMBL
Entry: F7V8L7_CLOSS
LinkDB: F7V8L7_CLOSS
Original site: F7V8L7_CLOSS 
ID   F7V8L7_CLOSS            Unreviewed;       424 AA.
AC   F7V8L7;
DT   21-SEP-2011, integrated into UniProtKB/TrEMBL.
DT   21-SEP-2011, sequence version 1.
DT   19-FEB-2014, entry version 16.
DE   RecName: Full=Phosphoribosylamine--glycine ligase;
DE            EC=6.3.4.13;
DE   AltName: Full=GARS;
DE   AltName: Full=Glycinamide ribonucleotide synthetase;
DE   AltName: Full=Phosphoribosylglycinamide synthetase;
GN   Name=PurD; Synonyms=purD; OrderedLocusNames=CXIVA_05100;
OS   Clostridium sp. (strain SY8519).
OC   Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=1042156;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SY8519;
RX   PubMed=21914882; DOI=10.1128/JB.05637-11;
RA   Yokoyama S., Oshima K., Nomura I., Hattori M., Suzuki T.;
RT   "Complete genomic sequence of the O-desmethylangolensin-producing
RT   bacterium Clostridium rRNA cluster XIVa strain SY8519, isolated from
RT   adult human intestine.";
RL   J. Bacteriol. 193:5568-5569(2011).
CC   -!- CATALYTIC ACTIVITY: ATP + 5-phospho-D-ribosylamine + glycine = ADP
CC       + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.
CC   -!- COFACTOR: Binds 1 magnesium or manganese ion per subunit (By
CC       similarity).
CC   -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway;
CC       N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-
CC       ribose 1-diphosphate: step 2/2.
CC   -!- SIMILARITY: Belongs to the GARS family.
CC   -!- SIMILARITY: Contains 1 ATP-grasp domain.
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DR   EMBL; AP012212; BAK46477.1; -; Genomic_DNA.
DR   RefSeq; YP_004707579.1; NC_015737.1.
DR   EnsemblBacteria; BAK46477; BAK46477; CXIVA_05100.
DR   GeneID; 10924400; -.
DR   KEGG; cls:CXIVA_05100; -.
DR   KO; K01945; -.
DR   BioCyc; CSP1042156:GHDR-532-MONOMER; -.
DR   UniPathway; UPA00074; UER00125.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0004637; F:phosphoribosylamine-glycine ligase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0009113; P:purine nucleobase biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.30.1490.20; -; 1.
DR   Gene3D; 3.30.470.20; -; 1.
DR   Gene3D; 3.40.50.20; -; 1.
DR   Gene3D; 3.90.600.10; -; 1.
DR   HAMAP; MF_00138; GARS; 1.
DR   InterPro; IPR011761; ATP-grasp.
DR   InterPro; IPR013815; ATP_grasp_subdomain_1.
DR   InterPro; IPR013816; ATP_grasp_subdomain_2.
DR   InterPro; IPR016185; PreATP-grasp_dom.
DR   InterPro; IPR020561; PRibGlycinamid_synth_ATP-grasp.
DR   InterPro; IPR000115; PRibGlycinamide_synth.
DR   InterPro; IPR020560; PRibGlycinamide_synth_C-dom.
DR   InterPro; IPR020559; PRibGlycinamide_synth_CS.
DR   InterPro; IPR020562; PRibGlycinamide_synth_N.
DR   InterPro; IPR011054; Rudment_hybrid_motif.
DR   Pfam; PF01071; GARS_A; 1.
DR   Pfam; PF02843; GARS_C; 1.
DR   Pfam; PF02844; GARS_N; 1.
DR   SUPFAM; SSF51246; SSF51246; 1.
DR   SUPFAM; SSF52440; SSF52440; 1.
DR   TIGRFAMs; TIGR00877; purD; 1.
DR   PROSITE; PS50975; ATP_GRASP; 1.
DR   PROSITE; PS00184; GARS; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Ligase; Magnesium; Manganese;
KW   Metal-binding; Nucleotide-binding; Purine biosynthesis.
FT   DOMAIN      107    313       ATP-grasp (By similarity).
FT   NP_BIND     133    194       ATP (By similarity).
FT   METAL       283    283       Magnesium or manganese (By similarity).
FT   METAL       285    285       Magnesium or manganese (By similarity).
SQ   SEQUENCE   424 AA;  46184 MW;  E9B6B1CCAC306F02 CRC64;
     MKVLIVGSGG REHAIAASVA KSSRVDKIYC APGNAGIGQI AELVPIGAME FDKLADFAKE
     NQIDLTIIGM DDPLVGGIVD VFEKEGLRVF GPRKNAAILE GSKAFSKDLM KKYKIPTAAY
     ETFDDPKEAL AYLETAPMPI VLKADGLALG KGVLICKTLE EARAGVKEIM EDKKFGSAGN
     HMVVEEFMTG REVSVLTYCD GKTIKIMSSA QDHKRAKDGD QGLNTGGMGT FSPSPFYTAE
     VDEFCRKYVF QPTVDAMAAE GREFKGIIFF GLMLTEDGPK VLEYNARFGD PEAQVVLPRM
     KNDIIDVMEA CIDGTLDQID LQFEDNAAVC VVLASDGYPV SYEKGFEISG LDEFDRHEGY
     YCFHAGTKRN EAGNFVTNGG RVLGVTAKGK TLQEARANAY AATEWVQFSN KYMRHDIGKA
     IDEA
//
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