ID F8F3H4_SPICH Unreviewed; 249 AA.
AC F8F3H4;
DT 21-SEP-2011, integrated into UniProtKB/TrEMBL.
DT 21-SEP-2011, sequence version 1.
DT 03-APR-2013, entry version 13.
DE RecName: Full=Ribonuclease 3;
DE EC=3.1.26.3;
DE AltName: Full=Ribonuclease III;
GN Name=rnc; OrderedLocusNames=Spica_1404;
OS Spirochaeta caldaria (strain ATCC 51460 / DSM 7334 / H1).
OC Bacteria; Spirochaetes; Spirochaetales; Spirochaetaceae; Spirochaeta.
OX NCBI_TaxID=744872;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51460 / DSM 7334 / H1;
RG US DOE Joint Genome Institute (JGI-PGF);
RA Lucas S., Han J., Lapidus A., Bruce D., Goodwin L., Pitluck S.,
RA Peters L., Kyrpides N., Mavromatis K., Ivanova N., Ovchinnikova G.,
RA Lu M., Misra M., Detter J.C., Tapia R., Han C., Land M., Hauser L.,
RA Markowitz V., Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Spring S.,
RA Schroeder M., Brambilla E., Klenk H.-P., Eisen J.A.;
RT "The complete genome of Spirochaeta caldaria DSM 7334.";
RL Submitted (JUN-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Digests double-stranded RNA. Involved in the processing
CC of primary rRNA transcript to yield the immediate precursors to
CC the large and small rRNAs (23S and 16S). Also processes some
CC mRNAs, and tRNAs when they are encoded in the rRNA operon (By
CC similarity).
CC -!- CATALYTIC ACTIVITY: Endonucleolytic cleavage to 5'-
CC phosphomonoester.
CC -!- COFACTOR: Mg(2+) (By similarity).
CC -!- SUBUNIT: Homodimer (By similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- SIMILARITY: Contains 1 DRBM (double-stranded RNA-binding) domain.
CC -!- SIMILARITY: Contains 1 RNase III domain.
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DR EMBL; CP002868; AEJ19550.1; -; Genomic_DNA.
DR RefSeq; YP_004698058.1; NC_015732.1.
DR EnsemblBacteria; AEJ19550; AEJ19550; Spica_1404.
DR GeneID; 10946819; -.
DR KEGG; scd:Spica_1404; -.
DR KO; K03685; -.
DR BioCyc; SCAL744872:GHIK-1447-MONOMER; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003725; F:double-stranded RNA binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004525; F:ribonuclease III activity; IEA:HAMAP.
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006397; P:mRNA processing; IEA:HAMAP.
DR GO; GO:0090305; P:nucleic acid phosphodiester bond hydrolysis; IEA:GOC.
DR GO; GO:0016075; P:rRNA catabolic process; IEA:InterPro.
DR GO; GO:0006364; P:rRNA processing; IEA:HAMAP.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 1.10.1520.10; -; 1.
DR Gene3D; 3.30.160.20; -; 1.
DR HAMAP; MF_00104; RNase_III; 1; -.
DR InterPro; IPR001159; Ds-RNA-bd.
DR InterPro; IPR014720; dsRNA-bd-like_dom.
DR InterPro; IPR011907; RNase_III.
DR InterPro; IPR000999; RNase_III_dom.
DR PANTHER; PTHR11207; PTHR11207; 1.
DR Pfam; PF00035; dsrm; 1.
DR Pfam; PF00636; Ribonuclease_3; 1.
DR SMART; SM00358; DSRM; 1.
DR SMART; SM00535; RIBOc; 1.
DR SUPFAM; SSF69065; RNase_III; 1.
DR TIGRFAMs; TIGR02191; RNaseIII; 1.
DR PROSITE; PS50137; DS_RBD; 1.
DR PROSITE; PS00517; RNASE_3_1; 1.
DR PROSITE; PS50142; RNASE_3_2; 1.
PE 3: Inferred from homology;
KW Complete proteome; Cytoplasm; Endonuclease; Hydrolase; Magnesium;
KW Metal-binding; mRNA processing; Nuclease; RNA-binding;
KW rRNA processing; rRNA-binding; tRNA processing.
FT DOMAIN 24 151 RNase III (By similarity).
FT DOMAIN 178 247 DRBM (By similarity).
FT ACT_SITE 68 68 By similarity.
FT ACT_SITE 140 140 By similarity.
FT METAL 64 64 Magnesium (By similarity).
FT METAL 137 137 Magnesium (By similarity).
FT METAL 140 140 Magnesium (By similarity).
SQ SEQUENCE 249 AA; 27880 MW; 10CFF4DC88C54012 CRC64;
MKDRSIQAVP AGSNTVNSAR KQVLIDFQKT AAIRFRSLEL LNLSFIHRSA SNEFPQKINN
ERLEFLGDAI LGACTATILY EKLSAQPEGE LAKIKSVVVS EDTLAGIARE LQIDVLLILG
KGEELSGGRT KKAILADALE ALIGAYYLDS GYKAVFEFIS KYLEVEIERV VTNQHHRDYK
TLLQEKCQQL YKNYPTYTLL KRTGPDHERM FWVEVHVNGV AYGPGIGKNK KEAEQEAAKL
AYKAITDIM
//
