GenomeNet

Database: UniProt/TrEMBL
Entry: F8GK77_NITSI
LinkDB: F8GK77_NITSI
Original site: F8GK77_NITSI 
ID   F8GK77_NITSI            Unreviewed;       400 AA.
AC   F8GK77;
DT   21-SEP-2011, integrated into UniProtKB/TrEMBL.
DT   21-SEP-2011, sequence version 1.
DT   19-FEB-2014, entry version 16.
DE   RecName: Full=Tyrosine--tRNA ligase;
DE            EC=6.1.1.1;
DE   AltName: Full=Tyrosyl-tRNA synthetase;
GN   Name=tyrS; OrderedLocusNames=Nit79A3_0683;
OS   Nitrosomonas sp. (strain Is79A3).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
OC   Nitrosomonadaceae; Nitrosomonas.
OX   NCBI_TaxID=261292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Is79A3;
RG   US DOE Joint Genome Institute;
RA   Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S.,
RA   Peters L., Ovchinnikova G., Lu M., Detter J.C., Han C., Tapia R.,
RA   Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., Bollmann A.,
RA   Norton J., Suwa Y., Klotz M., Stein L., Laanbroek H., Arp D.,
RA   Woyke T.;
RT   "Complete sequence of Nitrosomonas sp. Is79A3.";
RL   Submitted (JUN-2011) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a
CC       two-step reaction: tyrosine is first activated by ATP to form Tyr-
CC       AMP and then transferred to the acceptor end of tRNA(Tyr) (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: ATP + L-tyrosine + tRNA(Tyr) = AMP +
CC       diphosphate + L-tyrosyl-tRNA(Tyr).
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase
CC       family. TyrS type 2 subfamily.
CC   -!- SIMILARITY: Contains 1 S4 RNA-binding domain.
CC   -!- SIMILARITY: Contains S4 RNA-binding domain.
CC   -!- SIMILARITY: Contains SRNA-binding domain.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; CP002876; AEJ00562.1; -; Genomic_DNA.
DR   RefSeq; YP_004693961.1; NC_015731.1.
DR   EnsemblBacteria; AEJ00562; AEJ00562; Nit79A3_0683.
DR   GeneID; 10932767; -.
DR   KEGG; nii:Nit79A3_0683; -.
DR   KO; K01866; -.
DR   BioCyc; NSP261292:GH7H-696-MONOMER; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004831; F:tyrosine-tRNA ligase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0006437; P:tyrosyl-tRNA aminoacylation; IEA:UniProtKB-HAMAP.
DR   Gene3D; 3.10.290.10; -; 1.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_02007; Tyr_tRNA_synth_type2; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002305; aa-tRNA-synth_Ic.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR002307; Tyr-tRNA-ligase.
DR   InterPro; IPR024088; Tyr-tRNA-ligase_bac-type.
DR   InterPro; IPR024108; Tyr-tRNA-ligase_bac_2.
DR   PANTHER; PTHR11766; PTHR11766; 1.
DR   Pfam; PF00579; tRNA-synt_1b; 1.
DR   PRINTS; PR01040; TRNASYNTHTYR.
DR   SMART; SM00363; S4; 1.
DR   TIGRFAMs; TIGR00234; tyrS; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm;
KW   Ligase; Nucleotide-binding; Protein biosynthesis; RNA-binding.
FT   DOMAIN      338    399       S4 RNA-binding (By similarity).
FT   MOTIF        43     52       "HIGH" region (By similarity).
FT   MOTIF       227    231       "KMSKS" region (By similarity).
FT   BINDING     230    230       ATP (By similarity).
SQ   SEQUENCE   400 AA;  45129 MW;  62C55A97E28F5D1A CRC64;
     MNKSIKSQLE IIKRGSHELL VETELESKLI SGKPLRIKAG FDPTAPDLHL GHTVLLNKLR
     QLQDMGHHIL FLIGDFTGMI GDPSGKNTTR PPLTREQVAE NAKSYTAQVF KILKPEHTEV
     VFNSSWMDKL NAADLIKLAA THTVARMLER DDFDKRYSNN QAIAIHEFLY PLIQGYDSVA
     LRADLELGGT DQKFNLLMGR ELQKHFGQSQ QCILTMPLLE GLDGVNKMSK SLNNYVGITE
     SSKEIFGKLM SVSDQLMWRY FELLSFESLQ TIQKWREEVQ EGRNPRDIKV KLAQEIVVRF
     HSQQDAAEAL ADFEARFKHG ALPDEIPEKI IQAQDNEIPL VQILKLAGLT ASTSEALRMI
     DQGAVKLNGE KIEDKSTKIM RGESVVIQVG KRKFAKVVIQ
//
DBGET integrated database retrieval system