ID G0A2F7_METMM Unreviewed; 635 AA.
AC G0A2F7;
DT 19-OCT-2011, integrated into UniProtKB/TrEMBL.
DT 19-OCT-2011, sequence version 1.
DT 03-APR-2013, entry version 12.
DE RecName: Full=Threonine--tRNA ligase;
DE EC=6.1.1.3;
DE AltName: Full=Threonyl-tRNA synthetase;
GN Name=thrS; OrderedLocusNames=Metme_1719;
OS Methylomonas methanica (strain MC09).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales;
OC Methylococcaceae; Methylomonas.
OX NCBI_TaxID=857087;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=MC09;
RA Boden R., Cunliffe M., Scanlan J., Moussard H., Kits K.D., Klotz M.,
RA Jetten M., Vuilleumier S., Han J., Peters L., Mikhailova N.,
RA Teshima H., Tapia R., Kyrpides N., Ivanova N., Pagani I., Cheng J.-F.,
RA Goodwin L., Han C., Hauser L., Land M., Lapidus A., Lucas S.,
RA Pitluck S., Woyke T., Stein L.Y., Murrell C.;
RT "Complete genome sequence of the aerobic marine methanotroph
RT Methylomonas methanica MC09.";
RL Submitted (MAY-2011) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MC09;
RA Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S.,
RA Peters L., Mikhailova N., Teshima H., Han C., Tapia R., Land M.,
RA Hauser L., Kyrpides N., Ivanova N., Pagani I., Stein L., Woyke T.;
RT "Complete sequence of Methylomonas methanica MC09.";
RL Submitted (MAY-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY: ATP + L-threonine + tRNA(Thr) = AMP +
CC diphosphate + L-threonyl-tRNA(Thr).
CC -!- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
CC -!- SUBUNIT: Homodimer (By similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase
CC family.
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DR EMBL; CP002738; AEG00137.1; -; Genomic_DNA.
DR RefSeq; YP_004512636.1; NC_015572.1.
DR EnsemblBacteria; AEG00137; AEG00137; Metme_1719.
DR GeneID; 10671718; -.
DR KEGG; mmt:Metme_1719; -.
DR KO; K01868; -.
DR BioCyc; MMET857087:GH4A-1741-MONOMER; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004829; F:threonine-tRNA ligase activity; IEA:HAMAP.
DR GO; GO:0006435; P:threonyl-tRNA aminoacylation; IEA:HAMAP.
DR Gene3D; 3.10.20.30; -; 1.
DR Gene3D; 3.40.50.800; -; 1.
DR HAMAP; MF_00184; Thr_tRNA_synth; 1; -.
DR InterPro; IPR002314; aa-tRNA-synt_IIb_cons-dom.
DR InterPro; IPR006195; aa-tRNA-synth_II.
DR InterPro; IPR004154; Anticodon-bd.
DR InterPro; IPR012675; Beta-grasp_dom.
DR InterPro; IPR004095; TGS.
DR InterPro; IPR012676; TGS-like.
DR InterPro; IPR002320; Thr-tRNA-ligase_IIa.
DR InterPro; IPR018163; Thr/Ala-tRNA-synth_IIc_edit.
DR InterPro; IPR012947; tRNA_SAD.
DR Pfam; PF03129; HGTP_anticodon; 1.
DR Pfam; PF02824; TGS; 1.
DR Pfam; PF00587; tRNA-synt_2b; 1.
DR Pfam; PF07973; tRNA_SAD; 1.
DR PRINTS; PR01047; TRNASYNTHTHR.
DR SMART; SM00863; tRNA_SAD; 1.
DR SUPFAM; SSF52954; Anticodon_bd; 1.
DR SUPFAM; SSF81271; TGS-like; 1.
DR SUPFAM; SSF55186; Thr/Ala-tRNA-synth_IIc_edit; 1.
DR TIGRFAMs; TIGR00418; thrS; 1.
DR PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm;
KW Ligase; Metal-binding; Nucleotide-binding; Protein biosynthesis; Zinc.
FT REGION 242 533 Catalytic (By similarity).
FT METAL 333 333 Zinc; catalytic (By similarity).
FT METAL 384 384 Zinc; catalytic (By similarity).
FT METAL 510 510 Zinc; catalytic (By similarity).
SQ SEQUENCE 635 AA; 71775 MW; C3234E4BE853DEBC CRC64;
MPVITLPDGS QRQFDHAVTV MDVAQAIGSG LAKATLAGKV NDKLVDASTL IDSDASLQIV
TSKDEAGLEV IRHSTAHLLA QAVKQLFPSA QITIGPVIEN GFYYDIAFER SFTPEDLTDI
EKKMQELAAQ DIPVSRSLMS RDEAVNFFLG LGEKYKAEII ASIPANEDLS LYTQGDFTDL
CRGPHVPSTG KLKAFKLMKI AGAYWRGDSN NEMLQRIYGT AWTDGKELAA YLHRLEEAEK
RDHRKIGKTL DLFHTQEEAP GMVFWHDKGW AIYQQVEQYV REKLRVNGYG EVKTPQIVDR
SLWEKSGHWD KFSAMMFTTN SENRDYAVKP MNCPCHIQIY NQGIKSYRDL PVRLAEFGSC
HRNEPSGTLH GLMRVRNFVQ DDAHIFCTED QIQSEVATFI DLLFEVYKDF GFEEVIIKLS
TRPENRVGDD AVWDKSENAL ELALNTKGLD WQLQPGEGAF YGPKIEFSLK DCIGRVWQCG
TIQVDFSMPE RLGASYIGED SARHVPVMLH RAILGSLERF IGILIEQHAG NFPLWLAPVQ
LVVMNITDRH AEYAENVRLE LEKQGFRSKI DLRNEKIGFK IREHSMQRVP YLLIIGDKEL
DNQTVSVRTQ KGEDLGSLAV SALIERLNSD IADRK
//
