ID G0BL75_9ENTR Unreviewed; 314 AA.
AC G0BL75;
DT 19-OCT-2011, integrated into UniProtKB/TrEMBL.
DT 19-OCT-2011, sequence version 1.
DT 03-APR-2013, entry version 8.
DE RecName: Full=tRNA pseudouridine synthase B;
DE EC=5.4.99.25;
DE AltName: Full=tRNA pseudouridine(55) synthase;
DE AltName: Full=tRNA pseudouridylate synthase;
DE AltName: Full=tRNA-uridine isomerase;
GN Name=truB; ORFNames=SerAS12_0431;
OS Serratia sp. AS12.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Serratia.
OX NCBI_TaxID=768490;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=AS12;
RG US DOE Joint Genome Institute;
RA Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S.,
RA Peters L., Ovchinnikova G., Chertkov O., Han C., Tapia R., Land M.,
RA Hauser L., Kyrpides N., Ivanova N., Pagani I., Neupane S., Alstrom S.,
RA Finlay R., Hogberg N., Woyke T.;
RT "Complete sequence of Serratia sp. AS12.";
RL Submitted (MAY-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-
CC 55 in the psi GC loop of transfer RNAs (By similarity).
CC -!- CATALYTIC ACTIVITY: tRNA uridine(55) = tRNA pseudouridine(55).
CC -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family.
CC Type 1 subfamily.
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DR EMBL; CP002774; AEF48539.1; -; Genomic_DNA.
DR RefSeq; YP_004498896.1; NC_015566.1.
DR EnsemblBacteria; AEF48539; AEF48539; SerAS12_0431.
DR GeneID; 10624624; -.
DR KEGG; srs:SerAS12_0431; -.
DR KO; K03177; -.
DR GO; GO:0009982; F:pseudouridine synthase activity; IEA:HAMAP.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:HAMAP.
DR HAMAP; MF_01080; TruB_bact; 1; -.
DR InterPro; IPR002501; PsdUridine_synth.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom.
DR InterPro; IPR015947; PUA-like_domain.
DR InterPro; IPR014780; tRNA_psdUridine_synth_TruB.
DR InterPro; IPR015240; tRNA_sdUridine_synth_fam1_C.
DR PANTHER; PTHR13767; PTHR13767; 1.
DR Pfam; PF09157; TruB-C_2; 1.
DR Pfam; PF01509; TruB_N; 1.
DR SUPFAM; SSF55120; PsdUridine_synth_cat_dom; 1.
DR SUPFAM; SSF88697; PUA-like; 1.
DR TIGRFAMs; TIGR00431; TruB; 1.
PE 3: Inferred from homology;
KW Isomerase; tRNA processing.
FT ACT_SITE 48 48 Nucleophile (By similarity).
FT BINDING 43 43 Substrate (By similarity).
FT BINDING 76 76 Substrate (By similarity).
FT BINDING 179 179 Substrate (By similarity).
FT BINDING 200 200 Substrate (By similarity).
SQ SEQUENCE 314 AA; 35108 MW; 35269DF87BEF8B6F CRC64;
MSRPRRRGRD IHGVLLLDKP QGLSSNDALQ KVKRLYNANR AGHTGALDPL ATGMLPICLG
EATKFSQFLL DSDKRYRVIA KLGQRTDTSD ADGQIVQERP VNFTQTQLDT ALDSFRGDIK
QVPSMYSALK YQGKKLYEYA RQGIEVPREA RSITVYELQF IRWEGDELEL EIHCSKGTYI
RTITDDLGEL LGCGAHVIYL RRLQVATYPI ERMVTLEQLN ALLEQAQARE IAPGELLDPL
LMPMDSPVEN YPEVNLLPVV AGYVKQGQPV QVAGAPASGM VRMTEGEERK FIGVGDIADD
GRVAPRRLVV EHFD
//
