ID G3HKR8_CRIGR Unreviewed; 2412 AA.
AC G3HKR8;
DT 16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT 16-NOV-2011, sequence version 1.
DT 27-MAR-2024, entry version 77.
DE RecName: Full=[histone H3]-lysine(36) N-trimethyltransferase {ECO:0000256|ARBA:ARBA00012178};
DE EC=2.1.1.359 {ECO:0000256|ARBA:ARBA00012178};
DE AltName: Full=SET domain-containing protein 2 {ECO:0000256|ARBA:ARBA00030091};
GN ORFNames=I79_011298 {ECO:0000313|EMBL:EGV92157.1};
OS Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea;
OC Cricetidae; Cricetinae; Cricetulus.
OX NCBI_TaxID=10029 {ECO:0000313|EMBL:EGV92157.1, ECO:0000313|Proteomes:UP000001075};
RN [1] {ECO:0000313|Proteomes:UP000001075}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CHO K1 cell line {ECO:0000313|Proteomes:UP000001075};
RX PubMed=21804562; DOI=10.1038/nbt.1932;
RA Xu X., Nagarajan H., Lewis N.E., Pan S., Cai Z., Liu X., Chen W., Xie M.,
RA Wang W., Hammond S., Andersen M.R., Neff N., Passarelli B., Koh W.,
RA Fan H.C., Wang J., Gui Y., Lee K.H., Betenbaugh M.J., Quake S.R.,
RA Famili I., Palsson B.O., Wang J.;
RT "The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line.";
RL Nat. Biotechnol. 29:735-741(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-lysyl(36)-[histone H3] + 3 S-adenosyl-L-methionine = 3 H(+)
CC + N(6),N(6),N(6)-trimethyl-L-lysyl(36)-[histone H3] + 3 S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:60324, Rhea:RHEA-COMP:9785, Rhea:RHEA-
CC COMP:15536, ChEBI:CHEBI:15378, ChEBI:CHEBI:29969, ChEBI:CHEBI:57856,
CC ChEBI:CHEBI:59789, ChEBI:CHEBI:61961; EC=2.1.1.359;
CC Evidence={ECO:0000256|ARBA:ARBA00000317};
CC -!- SUBCELLULAR LOCATION: Chromosome {ECO:0000256|ARBA:ARBA00004286}.
CC Nucleus {ECO:0000256|ARBA:ARBA00004123}.
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DR EMBL; JH000468; EGV92157.1; -; Genomic_DNA.
DR RefSeq; XP_007632920.1; XM_007634730.2.
DR RefSeq; XP_016827946.1; XM_016972457.1.
DR RefSeq; XP_016832061.1; XM_016976572.1.
DR RefSeq; XP_016832062.1; XM_016976573.1.
DR STRING; 10029.G3HKR8; -.
DR PaxDb; 10029-XP_007632919-1; -.
DR eggNOG; KOG4442; Eukaryota.
DR InParanoid; G3HKR8; -.
DR Proteomes; UP000001075; Unassembled WGS sequence.
DR GO; GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0140955; F:histone H3K36 trimethyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:InterPro.
DR CDD; cd19172; SET_SETD2; 1.
DR CDD; cd00201; WW; 1.
DR Gene3D; 2.20.70.10; -; 1.
DR Gene3D; 1.20.930.10; Conserved domain common to transcription factors TFIIS, elongin A, CRSP70; 1.
DR Gene3D; 2.170.270.10; SET domain; 1.
DR Gene3D; 1.10.1740.100; Set2, Rpb1 interacting domain; 1.
DR InterPro; IPR006560; AWS_dom.
DR InterPro; IPR003616; Post-SET_dom.
DR InterPro; IPR001214; SET_dom.
DR InterPro; IPR046341; SET_dom_sf.
DR InterPro; IPR044437; SETD2/Set2_SET.
DR InterPro; IPR042294; SETD2_animal.
DR InterPro; IPR013257; SRI.
DR InterPro; IPR038190; SRI_sf.
DR InterPro; IPR035441; TFIIS/LEDGF_dom_sf.
DR InterPro; IPR001202; WW_dom.
DR InterPro; IPR036020; WW_dom_sf.
DR PANTHER; PTHR46711; HISTONE-LYSINE N-METHYLTRANSFERASE SETD2; 1.
DR PANTHER; PTHR46711:SF1; HISTONE-LYSINE N-METHYLTRANSFERASE SETD2; 1.
DR Pfam; PF17907; AWS; 1.
DR Pfam; PF00856; SET; 1.
DR Pfam; PF08236; SRI; 1.
DR Pfam; PF00397; WW; 1.
DR SMART; SM00570; AWS; 1.
DR SMART; SM00508; PostSET; 1.
DR SMART; SM00317; SET; 1.
DR SMART; SM00456; WW; 1.
DR SUPFAM; SSF82199; SET domain; 1.
DR SUPFAM; SSF51045; WW domain; 1.
DR PROSITE; PS51215; AWS; 1.
DR PROSITE; PS50868; POST_SET; 1.
DR PROSITE; PS50280; SET; 1.
DR PROSITE; PS01159; WW_DOMAIN_1; 1.
DR PROSITE; PS50020; WW_DOMAIN_2; 1.
PE 4: Predicted;
KW Chromatin regulator {ECO:0000256|ARBA:ARBA00022853};
KW Chromosome {ECO:0000256|ARBA:ARBA00022454};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:EGV92157.1}; Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000001075};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transcription {ECO:0000256|ARBA:ARBA00023163};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:EGV92157.1}.
FT DOMAIN 1343..1397
FT /note="AWS"
FT /evidence="ECO:0000259|PROSITE:PS51215"
FT DOMAIN 1399..1516
FT /note="SET"
FT /evidence="ECO:0000259|PROSITE:PS50280"
FT DOMAIN 1523..1539
FT /note="Post-SET"
FT /evidence="ECO:0000259|PROSITE:PS50868"
FT DOMAIN 2237..2270
FT /note="WW"
FT /evidence="ECO:0000259|PROSITE:PS50020"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 36..441
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 460..501
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 601..622
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 814..848
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 887..946
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1012..1077
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1160..1285
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1681..1717
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1768..1856
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1868..1985
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2287..2313
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..16
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 63..103
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 135..150
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 151..172
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 173..202
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 215..286
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 299..353
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 360..404
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 418..441
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 460..474
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 486..500
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 601..617
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 820..848
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 887..901
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 922..944
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1014..1029
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1030..1044
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1179..1194
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1195..1229
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1230..1281
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1681..1716
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1770..1798
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1801..1820
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1868..1896
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1903..1928
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1938..1978
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2297..2313
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2412 AA; 271848 MW; 0ADA2784E6A78DB1 CRC64;
MDTGDTFPTT EESSPPKSRV ELGRIHFKKH LLHVTSRPQL ATATSPPPTI QLPAVIAEST
VDSPPSSPPP PPPPPQATSP SPPASTSEPV ALPHPPVTVP MTAPVPLPVD SAQKEPPIKI
VPESLEVGMK QDIVSNSLEE HTIQTLREQA DNPLQREDSH IGKEEEVSDG SKTSLSSKKA
SSKKKSSQFE GTFLGSESDE DSVRTSSSQR SHDLKSSTSI EKERDFKKSS APSKSEDLGK
SSRSKTERDD KYFSYSKLER DTRYVSSRCR SERDRRRSRS RSRSDRVSRT SLSYSRSERS
HYYDSDRRYH RSSPYRERTR YSRPYTDNRA RESSDSEDEY KKTYPRRTSS HSYRDLRTSS
SYSKFDRDCK TETSYLEMER RGKYSSKLER ESKRTSDHEA IKRCCSPPTE LGFRRGSSYS
KHDNSASRYK SALSKPVSKS DKFKNSFCCT ELNEENKQSH FSLQTPCSKG SELRTSNKIP
EREKTGAPSP SNQLNDSPTF KKLDESPILK REFTEHDSHD SIKELESLSK VKNDQLRSFC
SIELNINGSP ETESDTVTFC ASKTDAFSLT SDDSVTGSEV SPLIKACMLS SNGFQNIGRC
KEDDSDDTYR QHNKSQSPFR ETEPLLLPHH DKLVSLPVKT IDYPKTLIKD PVDKRRSCCK
TKDSDRYCSP NENSEAENPE TSVVKISSNS FMNVHLESEI VLCDNTELTD QHSKCACDEY
KQSIGSTGSA SLNHFDDVYE SVGSSGIVSS LPSGVRCEEN TSPVLDAVQS KKSTEFLKYA
GKETEVGSAL PDSAKGFASW ENRYNNVLSG QSLQEAQEEG NSILHDRRGR TEISLDEEEG
RSRTHTSDDS EVVFSYDLNL TMEDSDSITY TLKCDSSGNI PEISTVHEDY SASSGSSSEE
SGSEDTESDD SSVPRHRLQS VVVVPKNSTL PMEETSPCSS RSSHSYRHYS DHWEDERLES
RRHSYEDKFE NMTSKGCPQT EKFFLHKGTE RNPETYSQFS RKVDNHLPEI AHSQSDGVDS
TSHTDLKSDS LGHLNSEDTV RAKTSRSQEL PVYSSDDFED IPNKSRQQMI FSNRPDSSRL
GKTEVNFSSC DISHMDGLHS SEELRNLGWD FSQQERPTTT YQQPDSSYGA CGTHKYQQSA
EHFAGTHSYW QGNGYWDPRS AGRPTGTGVA YDRIQGQVPD SLTDDREEES WDQRSGSHFP
SQSSKFLFHQ KDKGSVQAPE ISSNSVKDTL VPNERKEFSK NFEKNDIKER GPPKKRRQEV
ESDSESDGEL QARKKMRVES EPGETSVLLQ SELMGPSCAM EDFRDPQRWK EFAKLGKMPC
YFDLIEENVY LTERKKNKSH RDIKRMQCEC TPLSKDERAQ GEVACGEDCL NRLLMIECSS
RCPNGDYCSN RRFQRKQHAD VEVILTEKKG WGLRAAKDLP SNTFVLEYCG EVLDHKEFKA
RVKEYARNKN IHYYFMALKN DEIIDATQKG NCSRFMNHSC EPNCETQKWT VNGQLRVGFF
TTKLVPSGSE LTFDYQFQRY GKEAQKCFCG SANCRGYLGG ENRVSIRAAG GKMKKERSRK
KDSVDGELEA LMENGEGLSD KNQVLSLSRL MVRIETLEQK LTCLKLIQNT HSQSCLKSFL
ERHGLSLLWI WMAELGDGRE SNQKLQEEII KTLEHLPIPT KNMLEESKVL PIIQRWSQTK
TAVPPLSEGD GYSSENTSRA HTPLNTPDPS IKLSTEVDTE TPKKLVFRRL KIISENSMDS
AISDVTSELE CKDGKEDLDQ LETVTVEEDD DLQSQQLLPQ QPCESKVESE TTIEVSKLPT
SEPEADTEAE LKESSGTKLE ETIAEETPSQ DEEEGVSDVE SERSQEPPDK TVDISDLATK
LLDSWKDLKE VYRIPKKSQT EKESTVTERG RDAAAFRDQT VPKTPNRSRE RDPDKPTQNK
EKRKRRGSLS PPSSAYERGT KRPDDRYDTP TSKKKVRIKD RNKLSTEERR KLFEQEVAQR
EAQKQQQQMQ NLGMTSPLPF DSLGYNASHH PFAGYPPGYP MQAYVDPSNP NAGKVLLPTP
SMDPVCSPAP YDHAQPLVGH STESLAAPAS VPVVPHVAAS VEVSSSQYVA QNESVVHQDS
SVPVMPVQAP GPVQGQNYSV WDSNQQSVSV QQQYSPAQSQ ATIYYQGQTC STVYGVTSPY
SQTTPPIVQS YAQPNLQYIQ GQQIFTAHPQ GVVVQPTSAV TTIVAPGQPQ SLQPPEMVVT
NNLLDLPPPS PPKPKTIVLP PNWKTARDPE GKIYYYHVIT RQTQWDPPTW ESPGDDANLE
HEAEMDLGTP TYDENPMKTS KKPKTAEADT SSELAKKSKE VFRKEMSQFI VQCLNPYRKP
DCKVGRITTT EDFKHLARKL THGVMNKELK YCKNPEDLEC NENVKHKTKE YIKKYMQKFG
AVYKPKEDTE LE
//
