GenomeNet

Database: UniProt/TrEMBL
Entry: G8Q081_PSEFL
LinkDB: G8Q081_PSEFL
Original site: G8Q081_PSEFL 
ID   G8Q081_PSEFL            Unreviewed;       397 AA.
AC   G8Q081;
DT   22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT   22-FEB-2012, sequence version 1.
DT   05-JUL-2017, entry version 45.
DE   RecName: Full=Elongation factor Tu {ECO:0000256|HAMAP-Rule:MF_00118, ECO:0000256|RuleBase:RU004061};
DE            Short=EF-Tu {ECO:0000256|HAMAP-Rule:MF_00118};
GN   Name=tufB {ECO:0000313|EMBL:AEV65290.1};
GN   Synonyms=tuf {ECO:0000256|HAMAP-Rule:MF_00118};
GN   ORFNames=PSF113_5310 {ECO:0000313|EMBL:AEV65290.1};
OS   Pseudomonas fluorescens F113.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=1114970 {ECO:0000313|EMBL:AEV65290.1, ECO:0000313|Proteomes:UP000005437};
RN   [1] {ECO:0000313|EMBL:AEV65290.1, ECO:0000313|Proteomes:UP000005437}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=F113 {ECO:0000313|EMBL:AEV65290.1,
RC   ECO:0000313|Proteomes:UP000005437};
RX   PubMed=22328765; DOI=10.1128/JB.06601-11;
RA   Redondo-Nieto M., Barret M., Morrisey J.P., Germaine K.,
RA   Martinez-Granero F., Barahona E., Navazo A., Sanchez-Contreras M.,
RA   Moynihan J.A., Giddens S.R., Coppoolse E.R., Muriel C., Stiekema W.J.,
RA   Rainey P.B., Dowling D., O'Gara F., Martin M., Rivilla R.;
RT   "Genome Sequence of the Biocontrol Strain Pseudomonas fluorescens
RT   F113.";
RL   J. Bacteriol. 194:1273-1274(2012).
CC   -!- FUNCTION: This protein promotes the GTP-dependent binding of
CC       aminoacyl-tRNA to the A-site of ribosomes during protein
CC       biosynthesis. {ECO:0000256|HAMAP-Rule:MF_00118}.
CC   -!- SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_00118}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00118}.
CC   -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC       superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A
CC       subfamily. {ECO:0000256|HAMAP-Rule:MF_00118}.
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DR   EMBL; CP003150; AEV65290.1; -; Genomic_DNA.
DR   RefSeq; WP_003186103.1; NC_016830.1.
DR   STRING; 1114970.PSF113_5310; -.
DR   EnsemblBacteria; AEV65290; AEV65290; PSF113_5310.
DR   GeneID; 11833418; -.
DR   KEGG; pfe:PSF113_5310; -.
DR   PATRIC; fig|1114970.3.peg.5426; -.
DR   eggNOG; ENOG4105CGV; Bacteria.
DR   eggNOG; COG0050; LUCA.
DR   KO; K02358; -.
DR   Proteomes; UP000005437; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-HAMAP.
DR   CDD; cd03697; EFTU_II; 1.
DR   HAMAP; MF_00118_B; EF_Tu_B; 1.
DR   InterPro; IPR004161; EFTu-like_2.
DR   InterPro; IPR033720; EFTU_2.
DR   InterPro; IPR031157; G_TR_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR000795; TF_GTP-bd_dom.
DR   InterPro; IPR009000; Transl_B-barrel.
DR   InterPro; IPR009001; Transl_elong_EF1A/Init_IF2_C.
DR   InterPro; IPR004541; Transl_elong_EFTu/EF1A_bac/org.
DR   InterPro; IPR004160; Transl_elong_EFTu/EF1A_C.
DR   Pfam; PF00009; GTP_EFTU; 1.
DR   Pfam; PF03144; GTP_EFTU_D2; 1.
DR   Pfam; PF03143; GTP_EFTU_D3; 1.
DR   PRINTS; PR00315; ELONGATNFCT.
DR   SUPFAM; SSF50447; SSF50447; 1.
DR   SUPFAM; SSF50465; SSF50465; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00485; EF-Tu; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS00301; G_TR_1; 1.
DR   PROSITE; PS51722; G_TR_2; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000005437};
KW   Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00118};
KW   Elongation factor {ECO:0000256|HAMAP-Rule:MF_00118};
KW   GTP-binding {ECO:0000256|HAMAP-Rule:MF_00118};
KW   Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_00118};
KW   Protein biosynthesis {ECO:0000256|HAMAP-Rule:MF_00118};
KW   Reference proteome {ECO:0000313|Proteomes:UP000005437}.
FT   DOMAIN       10    207       Tr-type G (guanine nucleotide-binding).
FT                                {ECO:0000259|PROSITE:PS51722}.
FT   NP_BIND      19     26       GTP. {ECO:0000256|HAMAP-Rule:MF_00118}.
FT   NP_BIND      81     85       GTP. {ECO:0000256|HAMAP-Rule:MF_00118}.
FT   NP_BIND     136    139       GTP. {ECO:0000256|HAMAP-Rule:MF_00118}.
SQ   SEQUENCE   397 AA;  43586 MW;  D68D35FB6858F9C8 CRC64;
     MAKEKFERNK PHVNVGTIGH VDHGKTTLTA ALTRVCSEVF GSAKVDFDKI DSAPEEKARG
     ITINTAHVEY DSAVRHYAHV DCPGHADYVK NMITGAAQMD GAILVCSAAD GPMPQTREHI
     LLSRQVGVPY IVVFLNKADM VDDAELLELV EMEVRDLLST YDFPGDDTPI IIGSALMALN
     GQDDNEMGTT AVKKLVETLD SYIPEPERAI DKPFLMPIED VFSISGRGTV VTGRVERGIV
     RIQEEVEIVG LRDTQKTTCT GVEMFRKLLD EGRAGENCGV LLRGTKRDDV ERGQVLVKPG
     TVKPHTKFTA EVYVLSKEEG GRHTPFFKGY RPQFYFRTTD VTGNCELPEG VEMVMPGDNI
     QMTVTLIKTI AMEDGLRFAI REGGRTVGAG VVAKVIE
//
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