ID H2FTX2_OCESG Unreviewed; 315 AA.
AC H2FTX2;
DT 21-MAR-2012, integrated into UniProtKB/TrEMBL.
DT 21-MAR-2012, sequence version 1.
DT 01-MAY-2013, entry version 10.
DE RecName: Full=Glutathione synthetase;
DE EC=6.3.2.3;
DE AltName: Full=GSH synthetase;
DE AltName: Full=Glutathione synthase;
GN Name=gshB; OrderedLocusNames=GU3_08320;
OS Oceanimonas sp. (strain GK1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales;
OC Aeromonadaceae; Oceanimonas.
OX NCBI_TaxID=511062;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GK1;
RX DOI=10.1128/JB.00023-12;
RA Parsa Yeganeh L., Azarbaijani R., Sarikhan S., Mousavi H.,
RA Ramezani M., Amoozegar M.A., Shahzadeh Fazeli A., Salekdeh G.H.;
RT "Complete genome sequence of Oceanimonas sp. GK1, a halotolerant
RT bacterium from Gavkhouni Wetland in Iran.";
RL J. Bacteriol. 194:2123-2124(2012).
CC -!- CATALYTIC ACTIVITY: ATP + gamma-L-glutamyl-L-cysteine + glycine =
CC ADP + phosphate + glutathione.
CC -!- COFACTOR: Binds (magnesium or manganese,ion) per subunit (By
CC similarity).
CC -!- PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione
CC from L-cysteine and L-glutamate: step 2/2.
CC -!- SIMILARITY: Belongs to the prokaryotic GSH synthase family.
CC -!- SIMILARITY: Contains 1 ATP-grasp domain.
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DR EMBL; CP003171; AEY01420.1; -; Genomic_DNA.
DR RefSeq; YP_005092170.1; NC_016745.1.
DR EnsemblBacteria; AEY01420; AEY01420; GU3_08320.
DR GeneID; 11599637; -.
DR KEGG; oce:GU3_08320; -.
DR KO; K01920; -.
DR UniPathway; UPA00142; UER00210.
DR GO; GO:0005829; C:cytosol; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR GO; GO:0004363; F:glutathione synthase activity; IEA:HAMAP.
DR GO; GO:0000287; F:magnesium ion binding; IEA:HAMAP.
DR GO; GO:0030145; F:manganese ion binding; IEA:HAMAP.
DR Gene3D; 3.30.1490.20; -; 1.
DR Gene3D; 3.30.470.20; -; 1.
DR Gene3D; 3.40.50.20; -; 1.
DR HAMAP; MF_00162; GSH_S; 1; -.
DR InterPro; IPR011761; ATP-grasp.
DR InterPro; IPR013815; ATP_grasp_subdomain_1.
DR InterPro; IPR013816; ATP_grasp_subdomain_2.
DR InterPro; IPR006284; Glut_synth_pro.
DR InterPro; IPR004218; GSHS_ATP-bd.
DR InterPro; IPR004215; GSHS_N.
DR InterPro; IPR016185; PreATP-grasp_dom.
DR Pfam; PF02955; GSH-S_ATP; 1.
DR Pfam; PF02951; GSH-S_N; 1.
DR SUPFAM; SSF52440; PreATP-grasp-like; 1.
DR TIGRFAMs; TIGR01380; glut_syn; 1.
DR PROSITE; PS50975; ATP_GRASP; 1.
PE 3: Inferred from homology;
KW ATP-binding; Complete proteome; Glutathione biosynthesis; Ligase;
KW Magnesium; Manganese; Metal-binding; Nucleotide-binding.
FT DOMAIN 126 311 ATP-grasp (By similarity).
FT NP_BIND 152 208 ATP (By similarity).
FT METAL 282 282 Magnesium or manganese (By similarity).
FT METAL 284 284 Magnesium or manganese (By similarity).
SQ SEQUENCE 315 AA; 34927 MW; BE98A027140FF028 CRC64;
MTIKLGIVMD PIQSINIKKD TSFAMLLEAQ RRGYELWYME MSDLSLRDGE AFGDMARLSV
KEDASQWFSL GEPELKPLHS LDVILMRKDP PFDTEFIYAT YLLERAEGEG VLIVNKPQSL
RDANEKLYTA WFASHTPSTL VTRKAADIRA FHAEHKDIIM KPLDGMGGAS IFRVKEDDPN
LGVIIETLTE HGSRYCMVQT YLPAIKDGDK RVLVVDGEPV PYCLARIPQG GETRGNLAAG
GRGEARPLSD SDRRIAEALG PVLKSKGLIF VGLDIIGDKL TEVNVTSPTC VREIEAAFPV
NITGMLMDAI EKRLS
//
