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Database: UniProt/TrEMBL
Entry: H2FTX2_OCESG
LinkDB: H2FTX2_OCESG
Original site: H2FTX2_OCESG 
ID   H2FTX2_OCESG            Unreviewed;       315 AA.
AC   H2FTX2;
DT   21-MAR-2012, integrated into UniProtKB/TrEMBL.
DT   21-MAR-2012, sequence version 1.
DT   19-FEB-2014, entry version 14.
DE   RecName: Full=Glutathione synthetase;
DE            EC=6.3.2.3;
DE   AltName: Full=GSH synthetase;
DE   AltName: Full=Glutathione synthase;
GN   Name=gshB; OrderedLocusNames=GU3_08320;
OS   Oceanimonas sp. (strain GK1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales;
OC   Aeromonadaceae; Oceanimonas.
OX   NCBI_TaxID=511062;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GK1;
RX   PubMed=22461556; DOI=10.1128/JB.00023-12;
RA   Parsa Yeganeh L., Azarbaijani R., Sarikhan S., Mousavi H.,
RA   Ramezani M., Amoozegar M.A., Shahzadeh Fazeli A., Salekdeh G.H.;
RT   "Complete genome sequence of Oceanimonas sp. GK1, a halotolerant
RT   bacterium from Gavkhouni Wetland in Iran.";
RL   J. Bacteriol. 194:2123-2124(2012).
CC   -!- CATALYTIC ACTIVITY: ATP + gamma-L-glutamyl-L-cysteine + glycine =
CC       ADP + phosphate + glutathione.
CC   -!- COFACTOR: Binds 1 magnesium or manganese ion per subunit (By
CC       similarity).
CC   -!- PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione
CC       from L-cysteine and L-glutamate: step 2/2.
CC   -!- SIMILARITY: Belongs to the prokaryotic GSH synthase family.
CC   -!- SIMILARITY: Contains 1 ATP-grasp domain.
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DR   EMBL; CP003171; AEY01420.1; -; Genomic_DNA.
DR   RefSeq; YP_005092170.1; NC_016745.1.
DR   EnsemblBacteria; AEY01420; AEY01420; GU3_08320.
DR   GeneID; 11599637; -.
DR   KEGG; oce:GU3_08320; -.
DR   KO; K01920; -.
DR   OMA; PTCFQEI; -.
DR   BioCyc; OSP511062:GI6E-1735-MONOMER; -.
DR   UniPathway; UPA00142; UER00210.
DR   GO; GO:0005829; C:cytosol; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0004363; F:glutathione synthase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-HAMAP.
DR   Gene3D; 3.30.1490.20; -; 1.
DR   Gene3D; 3.30.470.20; -; 1.
DR   Gene3D; 3.40.50.20; -; 1.
DR   HAMAP; MF_00162; GSH_S; 1.
DR   InterPro; IPR011761; ATP-grasp.
DR   InterPro; IPR013815; ATP_grasp_subdomain_1.
DR   InterPro; IPR013816; ATP_grasp_subdomain_2.
DR   InterPro; IPR006284; Glut_synth_pro.
DR   InterPro; IPR004218; GSHS_ATP-bd.
DR   InterPro; IPR004215; GSHS_N.
DR   InterPro; IPR016185; PreATP-grasp_dom.
DR   Pfam; PF02955; GSH-S_ATP; 1.
DR   Pfam; PF02951; GSH-S_N; 1.
DR   SUPFAM; SSF52440; SSF52440; 1.
DR   TIGRFAMs; TIGR01380; glut_syn; 1.
DR   PROSITE; PS50975; ATP_GRASP; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Glutathione biosynthesis; Ligase;
KW   Magnesium; Manganese; Metal-binding; Nucleotide-binding.
FT   DOMAIN      126    311       ATP-grasp (By similarity).
FT   NP_BIND     152    208       ATP (By similarity).
FT   METAL       282    282       Magnesium or manganese (By similarity).
FT   METAL       284    284       Magnesium or manganese (By similarity).
SQ   SEQUENCE   315 AA;  34927 MW;  BE98A027140FF028 CRC64;
     MTIKLGIVMD PIQSINIKKD TSFAMLLEAQ RRGYELWYME MSDLSLRDGE AFGDMARLSV
     KEDASQWFSL GEPELKPLHS LDVILMRKDP PFDTEFIYAT YLLERAEGEG VLIVNKPQSL
     RDANEKLYTA WFASHTPSTL VTRKAADIRA FHAEHKDIIM KPLDGMGGAS IFRVKEDDPN
     LGVIIETLTE HGSRYCMVQT YLPAIKDGDK RVLVVDGEPV PYCLARIPQG GETRGNLAAG
     GRGEARPLSD SDRRIAEALG PVLKSKGLIF VGLDIIGDKL TEVNVTSPTC VREIEAAFPV
     NITGMLMDAI EKRLS
//
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