GenomeNet

Database: UniProt/TrEMBL
Entry: I7LLJ8_METBM
LinkDB: I7LLJ8_METBM
Original site: I7LLJ8_METBM 
ID   I7LLJ8_METBM            Unreviewed;       548 AA.
AC   I7LLJ8;
DT   03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT   03-OCT-2012, sequence version 1.
DT   25-OCT-2017, entry version 36.
DE   RecName: Full=DNA ligase {ECO:0000256|HAMAP-Rule:MF_00407};
DE            EC=6.5.1.1 {ECO:0000256|HAMAP-Rule:MF_00407};
DE   AltName: Full=Polydeoxyribonucleotide synthase [ATP] {ECO:0000256|HAMAP-Rule:MF_00407};
GN   Name=lig1 {ECO:0000313|EMBL:CCJ35449.1};
GN   Synonyms=lig {ECO:0000256|HAMAP-Rule:MF_00407};
GN   OrderedLocusNames=BN140_0526 {ECO:0000313|EMBL:CCJ35449.1};
OS   Methanoculleus bourgensis (strain ATCC 43281 / DSM 3045 / OCM 15 /
OS   MS2) (Methanogenium bourgense).
OC   Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
OC   Methanomicrobiaceae; Methanoculleus.
OX   NCBI_TaxID=1201294 {ECO:0000313|EMBL:CCJ35449.1, ECO:0000313|Proteomes:UP000009007};
RN   [1] {ECO:0000313|Proteomes:UP000009007}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43281 / DSM 3045 / OCM 15 / MS2
RC   {ECO:0000313|Proteomes:UP000009007};
RX   PubMed=22965103; DOI=10.1128/JB.01292-12;
RA   Maus I., Wibberg D., Stantscheff R., Eikmeyer F.G., Seffner A.,
RA   Boelter J., Szczepanowski R., Blom J., Jaenicke S., Konig H.,
RA   Puhler A., Schluter A.;
RT   "Complete genome sequence of the hydrogenotrophic, methanogenic
RT   archaeon Methanoculleus bourgensis strain MS2T, isolated from a sewage
RT   sludge digester.";
RL   J. Bacteriol. 194:5487-5488(2012).
CC   -!- FUNCTION: DNA ligase that seals nicks in double-stranded DNA
CC       during DNA replication, DNA recombination and DNA repair.
CC       {ECO:0000256|HAMAP-Rule:MF_00407}.
CC   -!- CATALYTIC ACTIVITY: ATP + (deoxyribonucleotide)(n)-3'-hydroxyl +
CC       5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) +
CC       AMP + diphosphate. {ECO:0000256|HAMAP-Rule:MF_00407}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_00407};
CC   -!- SIMILARITY: Belongs to the ATP-dependent DNA ligase family.
CC       {ECO:0000256|HAMAP-Rule:MF_00407, ECO:0000256|RuleBase:RU004196}.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; HE964772; CCJ35449.1; -; Genomic_DNA.
DR   RefSeq; WP_014866426.1; NC_018227.2.
DR   EnsemblBacteria; CCJ35449; CCJ35449; BN140_0526.
DR   GeneID; 13354133; -.
DR   KEGG; mbg:BN140_0526; -.
DR   PATRIC; fig|1201294.9.peg.565; -.
DR   KO; K10747; -.
DR   OMA; ETVCNIG; -.
DR   OrthoDB; POG093Z03L0; -.
DR   BioCyc; MBOU1201294:GLGD-526-MONOMER; -.
DR   Proteomes; UP000009007; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003910; F:DNA ligase (ATP) activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0071897; P:DNA biosynthetic process; IEA:InterPro.
DR   GO; GO:0051103; P:DNA ligation involved in DNA repair; IEA:InterPro.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.3260.10; -; 1.
DR   HAMAP; MF_00407; DNA_ligase; 1.
DR   InterPro; IPR022865; DNA_ligae_ATP-dep_bac/arc.
DR   InterPro; IPR000977; DNA_ligase_ATP-dep.
DR   InterPro; IPR012309; DNA_ligase_ATP-dep_C.
DR   InterPro; IPR012310; DNA_ligase_ATP-dep_cent.
DR   InterPro; IPR016059; DNA_ligase_ATP-dep_CS.
DR   InterPro; IPR012308; DNA_ligase_ATP-dep_N.
DR   InterPro; IPR036599; DNA_ligase_N_sf.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   Pfam; PF04679; DNA_ligase_A_C; 1.
DR   Pfam; PF01068; DNA_ligase_A_M; 1.
DR   Pfam; PF04675; DNA_ligase_A_N; 1.
DR   SUPFAM; SSF117018; SSF117018; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   TIGRFAMs; TIGR00574; dnl1; 1.
DR   PROSITE; PS00333; DNA_LIGASE_A2; 1.
DR   PROSITE; PS50160; DNA_LIGASE_A3; 1.
PE   3: Inferred from homology;
KW   ATP-binding {ECO:0000256|HAMAP-Rule:MF_00407};
KW   Cell cycle {ECO:0000256|HAMAP-Rule:MF_00407};
KW   Cell division {ECO:0000256|HAMAP-Rule:MF_00407};
KW   Complete proteome {ECO:0000313|Proteomes:UP000009007};
KW   DNA damage {ECO:0000256|HAMAP-Rule:MF_00407};
KW   DNA recombination {ECO:0000256|HAMAP-Rule:MF_00407};
KW   DNA repair {ECO:0000256|HAMAP-Rule:MF_00407};
KW   DNA replication {ECO:0000256|HAMAP-Rule:MF_00407};
KW   Ligase {ECO:0000256|HAMAP-Rule:MF_00407, ECO:0000313|EMBL:CCJ35449.1};
KW   Magnesium {ECO:0000256|HAMAP-Rule:MF_00407};
KW   Metal-binding {ECO:0000256|HAMAP-Rule:MF_00407};
KW   Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_00407};
KW   Reference proteome {ECO:0000313|Proteomes:UP000009007}.
FT   DOMAIN      322    446       DNA_LIGASE_A3. {ECO:0000259|PROSITE:
FT                                PS50160}.
FT   ACT_SITE    246    246       N6-AMP-lysine intermediate.
FT                                {ECO:0000256|HAMAP-Rule:MF_00407}.
FT   BINDING     244    244       ATP. {ECO:0000256|HAMAP-Rule:MF_00407}.
FT   BINDING     251    251       ATP. {ECO:0000256|HAMAP-Rule:MF_00407}.
FT   BINDING     266    266       ATP. {ECO:0000256|HAMAP-Rule:MF_00407}.
FT   BINDING     295    295       ATP. {ECO:0000256|HAMAP-Rule:MF_00407}.
FT   BINDING     334    334       ATP. {ECO:0000256|HAMAP-Rule:MF_00407}.
FT   BINDING     405    405       ATP. {ECO:0000256|HAMAP-Rule:MF_00407}.
FT   BINDING     411    411       ATP. {ECO:0000256|HAMAP-Rule:MF_00407}.
SQ   SEQUENCE   548 AA;  61152 MW;  CEA0573FE66125EF CRC64;
     MQFLEFARIC EHLEGISGRL DMIEQVSAVL PGLEDEELPI FVRFIMGRVF PDWSPKKLGV
     GPNLLYDAVA YVVGTKRSAV REAINATGDA GLAIEDLLAT KEQTSFFVQE MDLLEVYWDF
     ERVAAAEGAR SQREKLLVVR KLFANARPLE GRYLARLMLE ELRIGMGEGS VRDAVARAFD
     IDVRLVEHAH QAMNDLGEVA LLARRDPAAL SRVTIEPFRP VKMMLAQAGT IAGQIEDHGE
     VAVEYKYDGS RFQFHKVGNI CRIYSRRLEE VTGSLPDIVM HLGEATDHDV ILDGEVVAVQ
     DGRPMPFQYV IRRFRRKHQV NAMMEKIEVV PRVFDILYLD GETLMDRPLA ERRRILEEVL
     DAHIAPQFLV TDVAGAEAIY TEALNLGHEG VMVKVPSSPY TPGVRGRLWV KVKPGVETLD
     LAVIGAEWGE GRRAKMFGSF LLAVQDQGRL LPVGKVATGI TDEVLADLYA LLKDSVIARS
     GKEVTLEPKV VFEVGYSEIQ ASPNYASGYA LRFPRFVQVR EDKSVDEVET LDALAERYGE
     QKNGQGSL
//
DBGET integrated database retrieval system