ID K9XL93_9CHRO Unreviewed; 820 AA.
AC K9XL93;
DT 06-MAR-2013, integrated into UniProtKB/TrEMBL.
DT 06-MAR-2013, sequence version 1.
DT 27-MAR-2024, entry version 45.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=Glo7428_4004 {ECO:0000313|EMBL:AFZ32457.1};
OS Gloeocapsa sp. PCC 7428.
OC Bacteria; Cyanobacteriota; Cyanophyceae; Oscillatoriophycideae;
OC Chroococcales; Chroococcaceae; Gloeocapsa.
OX NCBI_TaxID=1173026 {ECO:0000313|EMBL:AFZ32457.1, ECO:0000313|Proteomes:UP000010476};
RN [1] {ECO:0000313|EMBL:AFZ32457.1, ECO:0000313|Proteomes:UP000010476}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 7428 {ECO:0000313|EMBL:AFZ32457.1,
RC ECO:0000313|Proteomes:UP000010476};
RG US DOE Joint Genome Institute;
RA Gugger M., Coursin T., Rippka R., Tandeau De Marsac N., Huntemann M.,
RA Wei C.-L., Han J., Detter J.C., Han C., Tapia R., Davenport K.,
RA Daligault H., Erkkila T., Gu W., Munk A.C.C., Teshima H., Xu Y., Chain P.,
RA Chen A., Krypides N., Mavromatis K., Markowitz V., Szeto E., Ivanova N.,
RA Mikhailova N., Ovchinnikova G., Pagani I., Pati A., Goodwin L., Peters L.,
RA Pitluck S., Woyke T., Kerfeld C.;
RT "Finished chromosome of genome of Gloeocapsa sp. PCC 7428.";
RL Submitted (JUN-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- SIMILARITY: Belongs to the N(4)/N(6)-methyltransferase family.
CC {ECO:0000256|ARBA:ARBA00006594}.
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DR EMBL; CP003646; AFZ32457.1; -; Genomic_DNA.
DR RefSeq; WP_015190325.1; NC_019745.1.
DR AlphaFoldDB; K9XL93; -.
DR STRING; 1173026.Glo7428_4004; -.
DR REBASE; 58149; M.Gsp7428ORF4004P.
DR KEGG; glp:Glo7428_4004; -.
DR PATRIC; fig|1173026.3.peg.4216; -.
DR eggNOG; COG0827; Bacteria.
DR HOGENOM; CLU_381660_0_0_3; -.
DR OrthoDB; 9798907at2; -.
DR Proteomes; UP000010476; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:InterPro.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:InterPro.
DR Gene3D; 3.40.1350.80; -; 1.
DR Gene3D; 1.10.10.1820; BsuBI/PstI restriction endonuclease-like; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR041963; BsuBI/PstI_C_sf.
DR InterPro; IPR041454; BsuBI/PstI_N.
DR InterPro; IPR041962; BsuBI/PstI_N_sf.
DR InterPro; IPR011639; MethylTrfase_TaqI-like_dom.
DR InterPro; IPR009528; Restrct_endonuc_II_BsuBI_C.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR Pfam; PF06616; BsuBI_PstI_RE; 1.
DR Pfam; PF17728; BsuBI_PstI_RE_N; 1.
DR Pfam; PF07669; Eco57I; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000313|EMBL:AFZ32457.1};
KW Methyltransferase {ECO:0000313|EMBL:AFZ32457.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000010476};
KW Transferase {ECO:0000313|EMBL:AFZ32457.1}.
FT DOMAIN 147..239
FT /note="Type II methyltransferase M.TaqI-like"
FT /evidence="ECO:0000259|Pfam:PF07669"
FT DOMAIN 514..651
FT /note="BsuBI/PstI restriction endonuclease HTH"
FT /evidence="ECO:0000259|Pfam:PF17728"
FT DOMAIN 661..814
FT /note="BsuBI/PstI restriction endonuclease"
FT /evidence="ECO:0000259|Pfam:PF06616"
SQ SEQUENCE 820 AA; 93151 MW; EAB07798992BE7A6 CRC64;
MATFLTDSAD IARVYYSSRL NLKQRSELGQ FLTPATVARF MAGQFNNLSG HIHLLDPGAG
IGTLTAAVVE RLLANPDQVS SCSITAYEIE PVFLPSLNQT LTECCDALSS KGVQADYCLR
EENFIKACSE MNLPLFKKVF SGFTHAILNP PYKKIHSQSA ERKVLSSIGI DTVNLYSAFV
WLATLQLIDD GEMVAITPRS FCNGTYFRPF RQAFLEYMKL EKIHIFESRA ATFSEDEILQ
ENVIFHAIKS KYKPTTVKIT SNSEVALDEI SESRYAPYDE VIEPNDSEQF IHIVTNSLKN
SLRVQMNQMP CTLDQLGLEV STGPVVDFRL KSALRSCLSD ENVPLIYPES IKTRKVVFPP
DNPRKPIAIE KNQDTKKWLM EPGWYVLTKR FSSKEEKRRV VAAVCPPTNS DFIGIENHLN
YYHSEGRGMT SDLAKGLAAF LNSTLFDCYF RQFSGHTQVN ATDLRRVKYP CKNDLIKIGV
QIGDNDSSQE EIDKVVHKVL SIMNEATTAV QAKKRIEEAL AILKTISAPR EQQNERSALC
LLALADIQPE KPWSQATAPR RGITEMMDWF RDHYGKQYAP NTRETVRRQT MHQFVQMGLV
VENPDKPDRP INSPKWCYQL HQQALSLLQV YGSEQWEEAR RNYAIAVTNL LQDRNRNIPM
IPISLPDGQA IQLSSGGQNI LIKDILESFC PRFTPGGVVL YIGDAGDKFI INETQKFREM
GIELEPHGKM PDVVIYHKQQ DWLVLIEAVT SHGPVNLKRH NELKQLFQSS RKGLVFVTAF
PSRREMTRYL AEISWETEVW VADQPDHMIH FNGERFLGPY
//
