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Database: UniProt/TrEMBL
Entry: Q0AHG9_NITEC
LinkDB: Q0AHG9_NITEC
Original site: Q0AHG9_NITEC 
ID   Q0AHG9_NITEC            Unreviewed;       316 AA.
AC   Q0AHG9;
DT   17-OCT-2006, integrated into UniProtKB/TrEMBL.
DT   17-OCT-2006, sequence version 1.
DT   26-NOV-2014, entry version 66.
DE   RecName: Full=Glutathione synthetase {ECO:0000256|HAMAP-Rule:MF_00162};
DE            EC=6.3.2.3 {ECO:0000256|HAMAP-Rule:MF_00162};
DE   AltName: Full=GSH synthetase {ECO:0000256|HAMAP-Rule:MF_00162};
DE   AltName: Full=Glutathione synthase {ECO:0000256|HAMAP-Rule:MF_00162};
GN   Name=gshB {ECO:0000256|HAMAP-Rule:MF_00162};
GN   OrderedLocusNames=Neut_0953 {ECO:0000313|EMBL:ABI59213.1};
OS   Nitrosomonas eutropha (strain C91).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
OC   Nitrosomonadaceae; Nitrosomonas.
OX   NCBI_TaxID=335283 {ECO:0000313|EMBL:ABI59213.1, ECO:0000313|Proteomes:UP000001966};
RN   [1] {ECO:0000313|EMBL:ABI59213.1, ECO:0000313|Proteomes:UP000001966}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C91 {ECO:0000313|EMBL:ABI59213.1,
RC   ECO:0000313|Proteomes:UP000001966};
RX   PubMed=17991028; DOI=10.1111/j.1462-2920.2007.01409.x;
RA   Stein L.Y., Arp D.J., Berube P.M., Chain P.S., Hauser L., Jetten M.S.,
RA   Klotz M.G., Larimer F.W., Norton J.M., Op den Camp H.J.M., Shin M.,
RA   Wei X.;
RT   "Whole-genome analysis of the ammonia-oxidizing bacterium,
RT   Nitrosomonas eutropha C91: implications for niche adaptation.";
RL   Environ. Microbiol. 9:2993-3007(2007).
CC   -!- CATALYTIC ACTIVITY: ATP + gamma-L-glutamyl-L-cysteine + glycine =
CC       ADP + phosphate + glutathione. {ECO:0000256|HAMAP-Rule:MF_00162}.
CC   -!- COFACTOR:
CC       Note=Binds 1 magnesium or manganese ion per subunit.
CC       {ECO:0000256|HAMAP-Rule:MF_00162};
CC   -!- PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione
CC       from L-cysteine and L-glutamate: step 2/2. {ECO:0000256|HAMAP-
CC       Rule:MF_00162}.
CC   -!- SIMILARITY: Belongs to the prokaryotic GSH synthase family.
CC       {ECO:0000256|HAMAP-Rule:MF_00162}.
CC   -!- SIMILARITY: Contains 1 ATP-grasp domain. {ECO:0000256|HAMAP-
CC       Rule:MF_00162}.
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DR   EMBL; CP000450; ABI59213.1; -; Genomic_DNA.
DR   RefSeq; WP_011634037.1; NC_008344.1.
DR   RefSeq; YP_747178.1; NC_008344.1.
DR   ProteinModelPortal; Q0AHG9; -.
DR   STRING; 335283.Neut_0953; -.
DR   EnsemblBacteria; ABI59213; ABI59213; Neut_0953.
DR   GeneID; 4272424; -.
DR   KEGG; net:Neut_0953; -.
DR   PATRIC; 22719091; VBINitEut7577_1212.
DR   eggNOG; COG0189; -.
DR   HOGENOM; HOG000265022; -.
DR   KO; K01920; -.
DR   OMA; WVRNYPE; -.
DR   OrthoDB; EOG6WMHWB; -.
DR   BioCyc; NEUT335283:GHT6-973-MONOMER; -.
DR   UniPathway; UPA00142; UER00210.
DR   GO; GO:0005829; C:cytosol; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0004363; F:glutathione synthase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-HAMAP.
DR   Gene3D; 3.30.1490.20; -; 1.
DR   Gene3D; 3.30.470.20; -; 1.
DR   Gene3D; 3.40.50.20; -; 1.
DR   HAMAP; MF_00162; GSH_S; 1.
DR   InterPro; IPR011761; ATP-grasp.
DR   InterPro; IPR013815; ATP_grasp_subdomain_1.
DR   InterPro; IPR013816; ATP_grasp_subdomain_2.
DR   InterPro; IPR006284; Glut_synth_pro.
DR   InterPro; IPR004218; GSHS_ATP-bd.
DR   InterPro; IPR004215; GSHS_N.
DR   InterPro; IPR016185; PreATP-grasp_dom.
DR   Pfam; PF02955; GSH-S_ATP; 1.
DR   Pfam; PF02951; GSH-S_N; 1.
DR   SUPFAM; SSF52440; SSF52440; 1.
DR   TIGRFAMs; TIGR01380; glut_syn; 1.
DR   PROSITE; PS50975; ATP_GRASP; 1.
PE   3: Inferred from homology;
KW   ATP-binding {ECO:0000256|HAMAP-Rule:MF_00162};
KW   Complete proteome {ECO:0000313|Proteomes:UP000001966};
KW   Glutathione biosynthesis {ECO:0000256|HAMAP-Rule:MF_00162};
KW   Ligase {ECO:0000256|HAMAP-Rule:MF_00162, ECO:0000313|EMBL:ABI59213.1};
KW   Magnesium {ECO:0000256|HAMAP-Rule:MF_00162};
KW   Manganese {ECO:0000256|HAMAP-Rule:MF_00162};
KW   Metal-binding {ECO:0000256|HAMAP-Rule:MF_00162};
KW   Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_00162}.
FT   DOMAIN      127    312       ATP-grasp. {ECO:0000256|HAMAP-Rule:
FT                                MF_00162}.
FT   NP_BIND     153    209       ATP. {ECO:0000256|HAMAP-Rule:MF_00162}.
FT   METAL       283    283       Magnesium or manganese.
FT                                {ECO:0000256|HAMAP-Rule:MF_00162}.
FT   METAL       285    285       Magnesium or manganese.
FT                                {ECO:0000256|HAMAP-Rule:MF_00162}.
SQ   SEQUENCE   316 AA;  35343 MW;  730671BCA8747ED4 CRC64;
     MKLAFILDPL DSIKIGKDSS YAMMCEAASR HHQLYTLQQS GLAWKNHQVI GFARSLTLID
     PPDNPHRWYE EGVIEEIPLT EFDAVLMRKD PPFDTEYIYS TYLLELAERQ GAYVVNSPRG
     IRDHNEKLAI TEFPQFIPSS LVTSQEQLIL QFLAEHKDII LKPLDGMGGA GIFRMQNTDH
     NIGVIIETLT RYGTRTIMAQ RFLPEIREGD KRILLIAGKP VDYALARIPK PGETRGNLAV
     GGTGKVQPLS ARDRKISEEL GQILYARGLM LIGLDVIGNH LTEINVTSPT GMREISDQTG
     INVAGLMIDA LEQNIH
//
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