ID   Q0K9K7_CUPNH            Unreviewed;       528 AA.
AC   Q0K9K7;
DT   03-OCT-2006, integrated into UniProtKB/TrEMBL.
DT   03-OCT-2006, sequence version 1.
DT   01-MAY-2013, entry version 48.
DE   RecName: Full=Malate synthase;
DE            EC=2.3.3.9;
GN   Name=aceB; OrderedLocusNames=H16_A2217;
OS   Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)
OS   (Ralstonia eutropha).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Cupriavidus.
OX   NCBI_TaxID=381666;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 17699 / H16 / DSM 428 / Stanier 337;
RX   PubMed=16964242; DOI=10.1038/nbt1244;
RA   Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H.,
RA   Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E.,
RA   Strittmatter A., Voss I., Gottschalk G., Steinbuechel A.,
RA   Friedrich B., Bowien B.;
RT   "Genome sequence of the bioplastic-producing 'Knallgas' bacterium
RT   Ralstonia eutropha H16.";
RL   Nat. Biotechnol. 24:1257-1262(2006).
CC   -!- CATALYTIC ACTIVITY: Acetyl-CoA + H(2)O + glyoxylate = (S)-malate +
CC       CoA.
CC   -!- PATHWAY: Carbohydrate metabolism; glyoxylate cycle; (S)-malate
CC       from isocitrate: step 2/2.
CC   -!- SIMILARITY: Belongs to the malate synthase family.
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DR   EMBL; AM260479; CAJ93314.1; -; Genomic_DNA.
DR   RefSeq; YP_726682.1; NC_008313.1.
DR   ProteinModelPortal; Q0K9K7; -.
DR   STRING; 381666.H16_A2217; -.
DR   EnsemblBacteria; CAJ93314; CAJ93314; H16_A2217.
DR   GeneID; 4247136; -.
DR   KEGG; reh:H16_A2217; -.
DR   PATRIC; 35233951; VBIRalEut6770_2623.
DR   eggNOG; COG2225; -.
DR   HOGENOM; HOG000238464; -.
DR   KO; K01638; -.
DR   OMA; RHVDIGD; -.
DR   ProtClustDB; PRK09255; -.
DR   BioCyc; CNEC381666:GJUJ-2185-MONOMER; -.
DR   UniPathway; UPA00703; UER00720.
DR   GO; GO:0004474; F:malate synthase activity; IEA:EC.
DR   GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   InterPro; IPR011076; Malate_synth-like.
DR   InterPro; IPR006252; Malate_synthA.
DR   InterPro; IPR001465; Malate_synthase.
DR   InterPro; IPR019830; Malate_synthase_CS.
DR   PANTHER; PTHR21631:SF1; PTHR21631:SF1; 1.
DR   Pfam; PF01274; Malate_synthase; 1.
DR   PIRSF; PIRSF001363; Malate_synth; 1.
DR   SUPFAM; SSF51645; Malat_synth_like; 1.
DR   TIGRFAMs; TIGR01344; malate_syn_A; 1.
DR   PROSITE; PS00510; MALATE_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Complete proteome; Glyoxylate bypass; Transferase;
KW   Tricarboxylic acid cycle.
FT   ACT_SITE    163    163       Proton acceptor (By similarity).
FT   ACT_SITE    445    445       Proton donor (By similarity).
SQ   SEQUENCE   528 AA;  59120 MW;  260AB657EF524308 CRC64;
     MAITLPAGMK ITGEILPAYE DILTPEALAL VDKLHRAFEP RRQELLAARV ERAKRLDAGE
     IPDFLPETKS IREGDWKVAP IPQALECRRV EITGPVEAKM VINAFNSGAD SYMTDFEDSN
     TPNWHNQLQG QVNLKAAVRR TLTLESKGKQ YKLNDKIATL QVRPRGWHLD EKHVTIDGKR
     ISGGIFDFAL FLFHNAREQI ARGAGPFFYL PKMESHLEAR LWNDIFVMAQ NEIGLPQGTI
     KATVLIETIL AAFEMDEILY ELREHSAGLN AGRWDYIFSC IKKFKNDKDF CLADRAKVTM
     TAPFMRAYAL LLLKTCHHRG APAMGGMSAL IPIKNDPEKN AIAMEGIISD KRRDATDGYD
     GGWVAHPGLV EPAMKEFVAV LGDKPNQFGK QRPDVQVKGA DLLDFKPETP ITEHGLRMNI
     NVGIHYLGAW LDGNGCVPIH NLMEDAATAE ISRSQVWQWI RSPKGKLEDG TKVTAELVRK
     LIPEELAKVK ALVGGDTKTY DRAAEIFEQM STSEDFAEFL TLPLYEEV
//