ID Q5WLX1_BACSK Unreviewed; 655 AA.
AC Q5WLX1;
DT 23-NOV-2004, integrated into UniProtKB/TrEMBL.
DT 23-NOV-2004, sequence version 1.
DT 01-MAY-2013, entry version 70.
DE RecName: Full=Methionine--tRNA ligase;
DE EC=6.1.1.10;
DE AltName: Full=Methionyl-tRNA synthetase;
GN Name=metS; Synonyms=metG; OrderedLocusNames=ABC0061;
OS Bacillus clausii (strain KSM-K16).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=66692;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KSM-K16;
RA Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y.,
RA Kawai S., Ito S., Horikoshi K.;
RT "The complete genome sequence of the alkaliphilic Bacillus clausii
RT KSM-K16.";
RL Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Is required not only for elongation of protein synthesis
CC but also for the initiation of all mRNA translation through
CC initiator tRNA(fMet) aminoacylation (By similarity).
CC -!- CATALYTIC ACTIVITY: ATP + L-methionine + tRNA(Met) = AMP +
CC diphosphate + L-methionyl-tRNA(Met).
CC -!- SUBUNIT: Homodimer (By similarity).
CC -!- SUBUNIT: Monomer (By similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- SIMILARITY: Contains 1 tRNA-binding domain.
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DR EMBL; AP006627; BAD62604.1; -; Genomic_DNA.
DR RefSeq; YP_173565.1; NC_006582.1.
DR ProteinModelPortal; Q5WLX1; -.
DR STRING; 66692.ABC0061; -.
DR EnsemblBacteria; BAD62604; BAD62604; ABC0061.
DR GeneID; 3204439; -.
DR KEGG; bcl:ABC0061; -.
DR PATRIC; 18919262; VBIBacCla58185_0064.
DR eggNOG; COG0143; -.
DR HOGENOM; HOG000200401; -.
DR KO; K01874; -.
DR OMA; RINYDLA; -.
DR ProtClustDB; PRK12267; -.
DR BioCyc; BCLA66692:GHMP-62-MONOMER; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR GO; GO:0004825; F:methionine-tRNA ligase activity; IEA:HAMAP.
DR GO; GO:0000049; F:tRNA binding; IEA:HAMAP.
DR GO; GO:0006431; P:methionyl-tRNA aminoacylation; IEA:HAMAP.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.40.50.620; -; 2.
DR HAMAP; MF_01228; Met_tRNA_synth_type2; 1; -.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR004495; Met-tRNA-synth_Ia_bsu_C.
DR InterPro; IPR014758; Met-tRNA_synth.
DR InterPro; IPR023457; Met-tRNA_synth_2.
DR InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR002547; tRNA-bd_dom.
DR InterPro; IPR009080; tRNAsynth_1a_anticodon-bd.
DR InterPro; IPR013155; V/L/I-tRNA-synth_anticodon-bd.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF09334; tRNA-synt_1g; 1.
DR Pfam; PF01588; tRNA_bind; 1.
DR PRINTS; PR01041; TRNASYNTHMET.
DR SUPFAM; SSF50249; Nucleic_acid_OB; 1.
DR SUPFAM; SSF47323; tRNAsyn_1a_bind; 1.
DR TIGRFAMs; TIGR00398; metG; 1.
DR TIGRFAMs; TIGR00399; metG_C_term; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
DR PROSITE; PS50886; TRBD; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm;
KW Ligase; Nucleotide-binding; Protein biosynthesis; RNA-binding;
KW tRNA-binding.
FT DOMAIN 555 655 tRNA-binding (By similarity).
FT MOTIF 13 23 "HIGH" region (By similarity).
FT MOTIF 309 313 "KMSKS" region (By similarity).
FT METAL 128 128 Zinc (By similarity).
FT METAL 161 161 Zinc (By similarity).
SQ SEQUENCE 655 AA; 74168 MW; B1B794E496B5BC58 CRC64;
MSEKTFYITT PIYYPSGKLH IGHTYTTVAG DAMARYKRLR GYDVRYLTGT DEHGQKIEQK
AKEKGISPLE YTNEMVKPIK ELWKKLDISH DDFIRTTEKR HTNMVEKIFE QLVEQGDIYL
GEYEGWYCVS DETFYGEREL VDPIKDESGN IIGGKSPDSG HPVEKVREKS YFFRMSKYAD
RLLAFYEENP DFIQPASRKN EMINNFIKPG LQDLAVSRTS FTWGVKVPSN PKHVVYVWID
ALTNYITALG YGTADDTLYQ KYWPADVHLV GKDILRFHTI YWPIMLMALD LPLPKKVFGH
GWFLTKDGKM SKSKGNVVDP APLIDHFGLD AIRYYLLREV PFGADGVFTP EAFVERVNYD
LANDLGNLLN RTVAMINKYF NGEIPAYVKS ASPFDEDLLD LVQATITKVE DAMEEMEFSI
ALAAIWQLVS RTNKYIDETQ PWMLAKEEDN RELLGSVMYH LAESLRIISV LIRPFMTRTP
AKIWAQLGID ESLAGWESIR VFGQLPAGTK VSKGEPVFPR LDVEKEVDYI VELMGGKADP
EPEEIEDENE ITIDDFSKVE LKVAEVTAAE PVEGADRLLK IQLDLGTETR QVVSGIAKYY
APSDLVGKKV ICVTNLKPVK LRGELSQGMI LAGSKGKKLQ LATVDGDLPN GAQVK
//
