ID Q5XLR3_DANRE Unreviewed; 707 AA.
AC Q5XLR3;
DT 23-NOV-2004, integrated into UniProtKB/TrEMBL.
DT 23-NOV-2004, sequence version 1.
DT 27-MAR-2024, entry version 70.
DE RecName: Full=Nicastrin {ECO:0000256|ARBA:ARBA00015303};
GN Name=ncstn {ECO:0000313|EMBL:AAV28475.1,
GN ECO:0000313|RefSeq:NP_001009556.1,
GN ECO:0000313|ZFIN:ZDB-GENE-050131-1};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955 {ECO:0000313|EMBL:AAV28475.1};
RN [1] {ECO:0000313|EMBL:AAV28475.1}
RP NUCLEOTIDE SEQUENCE.
RA Amsterdam A., Hopkins N.;
RT "The zebrafish nicastrin gene.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000000437}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=23594743; DOI=10.1038/nature12111;
RG Genome Reference Consortium Zebrafish;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Eliott D.,
RA Threadgold G., Harden G., Ware D., Begum S., Mortimore B., Mortimer B.,
RA Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M.,
RA Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M.,
RA Glithero R., Howden P., Barker N., Lloyd C., Stevens C., Harley J.,
RA Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D.,
RA Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K.,
RA Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R.,
RA Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M.,
RA Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D.,
RA Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M.,
RA Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M.,
RA Woodmansey R., Clark G., Cooper J., Cooper J., Tromans A., Grafham D.,
RA Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J.,
RA Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I.,
RA Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M.,
RA Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M.,
RA Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G., Osoegawa K., Zhu B.,
RA Rapp A., Widaa S., Langford C., Yang F., Schuster S.C., Carter N.P.,
RA Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R.,
RA Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I.,
RA Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H.,
RA Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [3] {ECO:0000313|RefSeq:NP_001009556.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=25940938;
RA Lim A., Moussavi Nik S.H., Ebrahimie E., Lardelli M.;
RT "Analysis of nicastrin gene phylogeny and expression in zebrafish.";
RL Dev. Genes Evol. 225:171-178(2015).
RN [4] {ECO:0000313|RefSeq:NP_001009556.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=28631837;
RA Shi W.J., Zhao J.L., Jiang Y.X., Huang G.Y., Liu Y.S., Zhang J.N.,
RA Ying G.G.;
RT "Transcriptional and histological alterations in gonad of adult zebrafish
RT after exposure to the synthetic progestin norgestrel.";
RL Environ. Toxicol. Chem. 36:3267-3276(2017).
RN [5] {ECO:0000313|RefSeq:NP_001009556.1}
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004479}; Single-
CC pass type I membrane protein {ECO:0000256|ARBA:ARBA00004479}.
CC -!- SIMILARITY: Belongs to the nicastrin family.
CC {ECO:0000256|ARBA:ARBA00007717}.
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DR EMBL; AY751744; AAV28475.1; -; mRNA.
DR RefSeq; NP_001009556.1; NM_001009556.1.
DR GeneID; 494449; -.
DR KEGG; dre:494449; -.
DR AGR; ZFIN:ZDB-GENE-050131-1; -.
DR CTD; 23385; -.
DR ZFIN; ZDB-GENE-050131-1; ncstn.
DR OrthoDB; 1330770at2759; -.
DR PhylomeDB; Q5XLR3; -.
DR Proteomes; UP000000437; Chromosome 2.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProt.
DR GO; GO:0032438; P:melanosome organization; IMP:ZFIN.
DR GO; GO:0007219; P:Notch signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0016485; P:protein processing; IEA:InterPro.
DR CDD; cd03881; M28_Nicastrin; 1.
DR Gene3D; 3.40.630.10; Zn peptidases; 1.
DR InterPro; IPR041084; Ncstrn_small.
DR InterPro; IPR008710; Nicastrin.
DR PANTHER; PTHR21092; NICASTRIN; 1.
DR PANTHER; PTHR21092:SF0; NICASTRIN; 1.
DR Pfam; PF18266; Ncstrn_small; 1.
DR Pfam; PF05450; Nicastrin; 1.
DR SUPFAM; SSF53187; Zn-dependent exopeptidases; 1.
PE 1: Evidence at protein level;
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Notch signaling pathway {ECO:0000256|ARBA:ARBA00022976};
KW Proteomics identification {ECO:0007829|PeptideAtlas:Q5XLR3};
KW Reference proteome {ECO:0000313|Proteomes:UP000000437};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT SIGNAL 1..24
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 25..707
FT /note="Nicastrin"
FT /evidence="ECO:0000256|SAM:SignalP,
FT ECO:0000313|RefSeq:NP_001009556.1"
FT /id="PRO_5035034142"
FT TRANSMEM 668..688
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 40..211
FT /note="Nicastrin small lobe"
FT /evidence="ECO:0000259|Pfam:PF18266"
SQ SEQUENCE 707 AA; 78161 MW; 6BABEBD7708EF648 CRC64;
MENLISINCV QLLITALFYT CISCSSVEQK IYVELNKTAP CVRLLNATHQ IGCQSSISGD
TGVVHVLESE SDLDWILSAG PHPPYMVIME TAFFNRSVML SMKNSSRVAG VAVIISKAGL
AEHFSPHTTC PNQNTGVYSA GSEFANCNGT VWNPLGNGLS YEDFTFPVFA LKDENQTQVI
RKCYEDHNLR VNGSAPQYPL CAMQLFSHMH AVTDTVTCMR RTDLQNKFSI NPEILCDPLS
DYNVWTSIRP LNNSARGHAE NESVVIAAVR LDGRSFFWEE APAAEGTVSA IVTLLAAIHA
LYPVIQEAPP LRNIFFTFFQ GEAFDYIGSS RMVFDMERNQ FVMDLDNVHS MLEIGQVGLR
GGRDVWIHSD PVSRRNSSME KQVSEMIKDV NSSAAGLNIN LQEPSVSQPL PPSSFQRFLR
VRPIPGLVLT DHEKAFNNRY YESMYDDAEN LNVSYPPNLT PDEQLVYPTD AAKSLAEVAT
LVARSLYTQA GGEQNNLSNI TADATMVSQL LYGFLVQKNN SWFRSLLPPE ITKKGGSSIL
SSGPPQFYIG LGPRLQSPVH SVTLFVQYIL ANLTGNVTNL TESQCQNPKE LSGENKDLYS
YFWVSGPSSG NSSADPLCVR ASVRLSKAVS PAFELLQYGS SDYSTWTESR WKSIRARIFL
VASRELEMLT LGVGVAVLLL SLLVTYFISS KAELLFSSAR ETPTTTY
//
